Application of creatinine-sensitive biosensor for hemodialysis control

Зінченко, О. А.; Марченко, С. В.; Sergeyeva, Т.А.; Кукла, А. Л.; Pavlyuchenko, A. S.; Krasyuk, E.; Солдаткин, А. П.; El'skaya, A. V.

doi:10.1016/j.bios.2012.02.062

Cited by 25 publications

(15 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The first example of a potentiometric biosensor for the detection of creatinine was proposed by Meyerhoff and Rechnitz, who used creatinine deiminase (CDI) coupled with an ammonia gas-sensing electrode (Meyerhoff and Rechnitz 1976). Latter, CDI immobilised in a bovine serum albumin (BSA) (Soldatkin et al 2002a) and PVA/SbQ membrane (Soldatkin et al 2002b) was used in combination with ion-sensitive field-effect transistors (ISFETs) for creatinine detection and hemodialysis control (Zinchenko et al 2012). However, these biosensors suffer from the inherent limitations of potentiometry, i.e.…”

Section: Introductionmentioning

confidence: 99%

Creatinine and urea biosensors based on a novel ammonium ion-selective copper-polyaniline nano-composite

Zhybak

Beni

Vagin

et al. 2016

Biosensors and Bioelectronics

105

View full text Add to dashboard Cite

The use of a novel ammonium ion-specific copper-polyaniline nano-composite as transducer for hydrolase-based biosensors is proposed. In this work, a combination of creatinine deaminase and urease has been chosen as a model system to demonstrate the construction of urea and creatinine biosensors to illustrate the principle. Immobilisation of enzymes was shown to be a crucial step in the development of the biosensors; the use of glycerol and lactitol as stabilisers resulted in a significant improvement, especially in the case of the creatinine, of the operational stability of the biosensors (from few hours to at least 3 days). The developed biosensors exhibited high selectivity towards creatinine and urea. The sensitivity was found to be 85 ± 3.4 mAM(-1)cm(-2) for the creatinine biosensor and 112 ± 3.36 mAM(-1)cm(-2) for the urea biosensor, with apparent Michaelis-Menten constants (KM,app), obtained from the creatinine and urea calibration curves, of 0.163 mM for creatinine deaminase and 0.139 mM for urease, respectively. The biosensors responded linearly over the concentration range 1-125 µM, with a limit of detection of 0.5 µM and a response time of 15s. The performance of the biosensors in a real sample matrix, serum, was evaluated and a good correlation with standard spectrophotometric clinical laboratory techniques was found.

show abstract

Section: Introductionmentioning

confidence: 99%

Creatinine and urea biosensors based on a novel ammonium ion-selective copper-polyaniline nano-composite

Zhybak

Beni

Vagin

et al. 2016

Biosensors and Bioelectronics

105

View full text Add to dashboard Cite

show abstract

“…5 Although majority of the existing sensors for creatinine estimation employ amperometric technique, increased complexity of the synergistic interaction among the multiple enzymes and a consequent decrease in its sensitivity limits its application as a point-of-care (POC) device. Hence, it is ideal to utilize a simple monoenzymatic pathway for creatinine estimation, [6][7][8] such as that involving creatinine deiminase, with no signicant interferences.…”

Section: Introductionmentioning

confidence: 99%

Biochemical assay for serum creatinine detection through a 1-methylhydantoin and cobalt complex

Dasgupta

Kumar²,

Krishnaswamy

et al. 2020

RSC Adv.

View full text Add to dashboard Cite

show abstract

“…Ammonium ion or H 2 O 2 , the final product of the hydrolysis reaction of creatinine catalyzed by one or three enzymes, is often measured to determine creatinine concentrations. Transducers such as amperometry [ 21 , 22 ], potentiometry [ 23 , 24 ], ion-sensitive field effect transistor [ 25 , 26 , 27 ], or spectrophotometry [ 28 ] may be used based on the type of the final reaction products. Among these biosensors, the amperometric method was the first system to be successfully commercialized [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Development of Ratiometric Fluorescent Biosensors for the Determination of Creatine and Creatinine in Urine

Duong

Rhee

2017

Sensors

View full text Add to dashboard Cite

In this study, the oxazine 170 perchlorate (O17)-ethylcellulose (EC) membrane was successfully exploited for the fabrication of creatine- and creatinine-sensing membranes. The sensing membrane exhibited a double layer of O17-EC membrane and a layer of enzyme(s) entrapped in the EC and polyurethane hydrogel (PU) matrix. The sensing principle of the membranes was based on the hydrolytic catalysis of urea, creatine, and creatinine by the enzymes. The reaction end product, ammonia, reacted with O17-EC membrane, resulting in the change in fluorescence intensities at two emission wavelengths (λem = 565 and 625 nm). Data collected from the ratio of fluorescence intensities at λem = 565 and 625 nm were proportional to the concentrations of creatine or creatinine. Creatine- and creatinine-sensing membranes were very sensitive to creatine and creatinine at the concentration range of 0.1–1.0 mM, with a limit of detection (LOD) of 0.015 and 0.0325 mM, respectively. Furthermore, these sensing membranes showed good features in terms of response time, reversibility, and long-term stability. The interference study demonstrated that some components such as amino acids and salts had some negative effects on the analytical performance of the membranes. Thus, the simple and sensitive ratiometric fluorescent sensors provide a simple and comprehensive method for the determination of creatine and creatinine concentrations in urine.

show abstract

Application of creatinine-sensitive biosensor for hemodialysis control

Cited by 25 publications

References 23 publications

Creatinine and urea biosensors based on a novel ammonium ion-selective copper-polyaniline nano-composite

Creatinine and urea biosensors based on a novel ammonium ion-selective copper-polyaniline nano-composite

Biochemical assay for serum creatinine detection through a 1-methylhydantoin and cobalt complex

Development of Ratiometric Fluorescent Biosensors for the Determination of Creatine and Creatinine in Urine

Contact Info

Product

Resources

About