Biochemical assay for serum creatinine detection through a 1-methylhydantoin and cobalt complex

Dasgupta, Pallavi; Kumar, Vanita; Krishnaswamy, P. R.; Bhat, Navakanta

doi:10.1039/d0ra06470j

Cited by 12 publications

(5 citation statements)

References 14 publications

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“…Further, the sensor was tested in a serum sample that showed a recovery between 95% and 98%. Another enzymatic electrochemical sensor for the detection of creatinine has been developed by Dasgupta et al, (Dasgupta et al, 2020). Here, the CD was used for the conversion of creatinine to N‐methylhydantoin (shown in Figure 4c,ii) and the amount of 1‐methylhydantoin utilized for indirect estimation of different creatinine concentrations.…”

Section: Electrochemical Sensors For Detection Of Ckdmentioning

confidence: 99%

“…(a) Schematic representation of fabricated electrode using dispenser for the creatinine detection, Reproduce with permission (Boobphahom et al, 2019) (b) Illustration of modified electrode through copper electrodeposition for the determination of creatinine in buffer with and without analyte through voltammetric analysis, Reproduce with permission (Raveendran et al, 2017) (c) (i) Representation of modified electrode and redox mechanism (ii) Showing conversion of creatinine with enzyme and the complete reaction mechanism in solution (iii) effect of presence and absence of target molecule on signal generation, Reproduce with permission (Dasgupta et al, 2020). …”

Section: Electrochemical Sensors For Detection Of Ckdmentioning

confidence: 99%

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Design and development of opto‐electrochemical biosensing devices for diagnosing chronic kidney disease

Kumar

Darshna

Sammi

et al. 2023

Biotech & Bioengineering

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Chronic kidney disease (CKD) is emerging as one of the major causes of the increase in mortality rate and is expected to become 5th major cause by 2050. Many studies have shown that it is majorly related to various risk factors, and thus becoming one of the major health issues around the globe. Early detection of renal disease lowers the overall burden of disease by preventing individuals from developing kidney impairment. Therefore, diagnosis and prevention of CKD are becoming the major challenges, and in this situation, biosensors have emerged as one of the best possible solutions. Biosensors are becoming one of the preferred choices for various diseases diagnosis as they provide simpler, cost‐effective and precise methods for onsite detection. In this review, we have tried to discuss the globally developed biosensors for the detection of CKD, focusing on their design, pattern, and applicability in real samples. Two major classifications of biosensors based on transduction systems, that is, optical and electrochemical, for kidney disease have been discussed in detail. Also, the major focus is given to clinical biomarkers such as albumin, creatinine, and others related to kidney dysfunction. Furthermore, the globally developed sensors for the detection of CKD are discussed in tabulated form comparing their analytical performance, response time, specificity as well as performance in biological fluids.

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Section: Electrochemical Sensors For Detection Of Ckdmentioning

confidence: 99%

Section: Electrochemical Sensors For Detection Of Ckdmentioning

confidence: 99%

Design and development of opto‐electrochemical biosensing devices for diagnosing chronic kidney disease

Kumar

Darshna

Sammi

et al. 2023

Biotech & Bioengineering

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“…Creatine, a source of energy in the form of phosphate, can be found in the muscles of a healthy individual [1]. Creatine produces a nitrogenous waste called creatinine that is excreted through the kidneys by glomerular filtration [2].…”

Section: Introductionmentioning

confidence: 99%

ZIF-8 Nanoparticles Based Electrochemical Sensor for Non-Enzymatic Creatinine Detection

et al. 2022

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There is a consistent demand for developing highly sensitive, stable, cost-effective, and easy-to-fabricate creatinine sensors as creatinine is a reliable indicator of kidney and muscle-related disorders. Herein, we reported a highly sensitive and selective non-enzymatic electrochemical creatinine sensor via modifying poly(3,4 ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) coated indium tin oxide (ITO) substrate by zeolitic imidazolate framework-8 nanoparticles (ZIF-8 NPs). The topography, crystallinity, and composition of the sensing electrode were characterized by field emission scanning electron microscopy (FESEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The peroxidase-like activity of ZIF-8 nanoparticles enabled it to detect creatinine forming a zinc-creatinine composite. The electrochemical behavior and sensing performance were evaluated by amperometric and impedimetric analysis. The sensor obtained a sufficiently low limit of detection (LOD) of 30 µM in a clinically acceptable linear range (0.05 mM–2.5 mM). The interference study demonstrated high selectivity of the sensor for creatinine concerning other similar biomolecules. The sensing performance of the creatinine sensor was verified in the actual human serum, which showed excellent recovery rates. Hence, the magnificent performance of ZIF-8 based non-enzymatic creatinine sensor validated it as a responsible entity for other complicated renal markers detection.

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“…Several well-established methods, such as colorimetric, spectroscopic, and chromatographic techniques, have been widely used for creatinine detection. − However, tedious sample preparation, especially in colorimetric detection, and the requirement of sophisticated laboratory equipment, including lack of accurate quantitative measurement, restricts their applicability for rapid on-site monitoring and point of care diagnosis. On the other hand, electrochemical quantification using enzymatic biosensors represents a feasible laboratory-scale measurement technique. − However, the methods became complicated and less reliable because the immobilization of enzymes affects the biosensor shelf life in storage and operational stability.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the development of simple and effective nonenzymatic techniques similar to the clinical diagnosis kits devices for the real-time monitoring of glucose is an important goal for point of care (POC) measurement of creatinine levels. A summary of prior work conducted for nonenzymatic electrochemical detection of creatinine and the gaps in these techniques are shown in Table S1. ,− As mentioned in Table S1, the existing literature reveals that although each exploration involves a unique creatinine detection method, significant gaps still exist. They are as follows: (i) the current responses of the sensors are low in the microamps range and (ii) the lowest and highest creatinine levels in human blood so far reported are out of detection limits.…”

Section: Introductionmentioning

confidence: 99%

Facile Detection of Blood Creatinine Using Binary Copper–Iron Oxide and rGO-Based Nanocomposite on 3D Printed Ag-Electrode under POC Settings

Singh

Mandal

Roy

et al. 2021

ACS Biomater. Sci. Eng.

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Metal nanoparticles have been helpful in creatinine sensing technology under point-of-care (POC) settings because of their excellent electrocatalyst properties. However, the behavior of monometallic nanoparticles as electrochemical creatinine sensors showed limitations concerning the current density in the mA/cm2 range and wide detection window, which are essential parameters for the development of a sensor for POC applications. Herein, we report a new sensor, a reduced graphene oxide stabilized binary copper–iron oxide-based nanocomposite on a 3D printed Ag-electrode (Fe–Cu–rGO@Ag) for detecting a wide range of blood creatinine (0.01 to 1000 μM; detection limit 10 nM) in an electrochemical chip with a current density ranging between 0.185 and 1.371 mA/cm2 and sensitivity limit of 1.1 μA μM–1 cm–2 at physiological pH. Interference studies confirmed that the sensor exhibited no interference from analytes like uric acid, urea, dopamine, and glutathione. The sensor response was also evaluated to detect creatinine in human blood samples with high accuracy in less than a minute. The sensing mechanism suggested that the synergistic effects of Cu and iron oxide nanoparticles played an essential role in the efficient sensing where Fe atoms act as active sites for creatinine oxidation through the secondary amine nitrogen, and Cu nanoparticles acted as an excellent electron-transfer mediator through rGO. The rapid sensor fabrication procedure, mA/cm2 peak current density, a wide range of detection limits, low contact resistance including high selectivity, excellent linear response (R 2 = 0.991), and reusability ensured the application of advanced electrochemical sensor toward the POC creatinine detection.

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Biochemical assay for serum creatinine detection through a 1-methylhydantoin and cobalt complex

Abstract: A highly sensitive amperometric biosensor has been developed for creatinine estimation using the mono-enzymatic conversion of creatinine by creatinine deiminase.

Cited by 12 publications

References 14 publications

Design and development of opto‐electrochemical biosensing devices for diagnosing chronic kidney disease

Design and development of opto‐electrochemical biosensing devices for diagnosing chronic kidney disease

ZIF-8 Nanoparticles Based Electrochemical Sensor for Non-Enzymatic Creatinine Detection

Facile Detection of Blood Creatinine Using Binary Copper–Iron Oxide and rGO-Based Nanocomposite on 3D Printed Ag-Electrode under POC Settings

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