Application of customised bacterial inoculants for grass haylage production and its effectiveness on nutrient composition and fermentation quality of haylage

Abstract

The present study aimed to investigate the efficacy of customised KCC-10, KCC-19 and K-46 on nutrient composition and fermentation quality of low moisture Italian ryegrass (IRG) forage. An addition of customised bacterial inoculants (CBI) did not affect the nutrient compositions and digestibility rates of haylage. The lactic acid content was higher in CBI-inoculated haylage, whereas the amount of acetic acid and butyric acid production was significantly reduced than the control. CBI-inoculated haylage exhibited higher numbers of bacterial colonies that reduced the pH of the haylage. Low pH in haylage is an important criterion for preventing undesirable microbial growth and improves fermentation quality of haylage. PCR studies indicated that the DNA of was predominantly amplified. It evidenced that the CBI is the main reason behind the improvement of haylage fermentation as compared to control. Overall results suggested that KCC-10, KCC-19 and K-46 are considered as potent strains for improving fermentation quality of low moisture forage and preserve its stability for a long time.

Probiotic and Triticale Silage Fermentation Potential of Pediococcus pentosaceus and Lactobacillus brevis and Their Impacts on Pathogenic Bacteria

“…Recently LAB can be considered as important additives to preserve the silage for a long time and improving its quality via increasing lactic acid production. Also, the addition of LAB prevents the undesirable microbial growth in silage by reducing the pH of the surrounding places [18,19].…”

“…The spoilage microorganisms not only affect the nutritional quality of silage but also influence animal health and its products. LAB has long been used as inoculums to improve low/high moisture silage quality worldwide and also in improving milk production, increasing body weight and efficiency of feed intake [18,19]. Interestingly, LAB exerts antibacterial and antifungal activities against human and animal pathogens by its secondary metabolites [20,21,22].…”

Application of Lactobacillus inoculant from various rice paddy (Oryza sativa) to total mixed ration silage microbial composition

“…Yeasts and filamentous mold were the main microorganisms involved in the aerobic deterioration process of silages (Santos et al 2013b). When selecting a potent LAB for silage production that LAB should have characteristics of rapid growth (high cell density at ensiled silages), higher production of lactic acid with a marginal level of acetic acid, potent inhibition of pathogens (bacteria, yeast, and toxic metabolite producing mold), rapid reduction in pH, increased aerobic stability and dry matter content (DM), and long-term preservation with rich nutrients (Carvalho et al 2021;Soundharrajan et al 2017;Shah et al 2021). Lactic acid bacteria.…”

Application and Future Prospective of Lactic Acid Bacteria as Natural Additives for Silage Production—A Review

“…Lactic acid bacteria play a role in the conversion of water-soluble carbohydrates into organic acids; it induces the acidification of silages and kills or prevents detrimental microbial growth. When selecting a potent LAB for silage production that LAB should have the following criteria; rapid growth (high cell density at ensiled silages), rapid reduction in pH, potent inhibition of pathogens (bacteria, yeast, and toxic metabolite producing mold), higher production of lactic acid with a marginal level of acetic acid, very low or complete inhibition of production of butyric acid content (a negative indicator of silages), increased aerobic stability and dry matter content (DM), and long-term preservation with rich nutrients [61,103,104].…”