Application of Cytosense flow cytometer for the analysis of airborne bacteria collected by a high volume impingement sampler

Jang, Jaeyoun; Hendriksen, Niels Bohse; Jakobsen, Hans Henrik; Gosewinkel, Ulrich

doi:10.1016/j.mimet.2018.10.012

Cited by 6 publications

(4 citation statements)

References 45 publications

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“…The quantification of bacterial concentrations ( c cells ) in 7 snow and 2 air samples was performed by flow cytometry , using a NovoCyte ACEA (Biosciences Inc., LOD: 0.2–50 mm cell size). Flow cytometry is a technique that quantifies cells, which can be labeled with fluorescent markers, in a liquid suspension.…”

Section: Methodsmentioning

confidence: 99%

Biogenic Sources of Ice Nucleating Particles at the High Arctic Site Villum Research Station

Šantl-Temkiv

Lange

Beddows

et al. 2019

Environ. Sci. Technol.

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The radiative balance in the Arctic region is sensitive to in-cloud processes, which principally depend on atmospheric aerosols, including ice nucleating particles (INPs). High temperature INPs (active at ≥−15 °C) are common in the Arctic. While laboratory and limited in situ studies show that the high-temperature active INPs are associated with bioaerosols and biogenic compounds, there is still little quantitative insight into the Arctic biogenic INPs and bioaerosols. We measured concentrations of bioaerosols, bacteria, and biogenic INPs at the Villum Research Station (VRS, Station Nord) in a large number of snow (15) and air (51) samples. We found that INPs active at high subzero temperatures were present both in spring and summer. Air INP concentrations were higher in summer (18 INP m −3 at ≥−10 °C) than in spring (<4 INP m −3 at ≥−10 °C), when abundant INPs were found in snowfall (1.4 INP mL −1 at ≥−10 °C). Also, in summer, a significantly higher number of microbial and bacterial cells were present compared to the spring. A large proportion (60%−100%) of INPs that were active between −6 °C and −20 °C could be deactivated by heating to 100 °C, which was indicative of their predominantly proteinaceous origin. In addition, there was a significant linear regression between the summer air concentrations of INPs active at ≥−10 °C and air concentrations of bacterial-marker-genes (p < 0.0001, R 2 = 0.999, n = 6), pointing at bacterial cells as the source of high-temperature active INPs. In conclusion, the majority of INPs was of proteinaceous, and possibly of bacterial, origin and was found in air during summer and in snowfall during springtime.

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Section: Methodsmentioning

confidence: 99%

Biogenic Sources of Ice Nucleating Particles at the High Arctic Site Villum Research Station

Šantl-Temkiv

Lange

Beddows

et al. 2019

Environ. Sci. Technol.

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“…On two of the visits, the sampling procedure was performed twice (morning and evening) resulting in a total of seven samplings included in the investigation. Bioaerosols were collected from the outlets of the ventilation system of the farm and from the area around the farm by Kärchner DS5800 vacuum-cleaners functioning as impingers as described earlier (12,13). The sampling was performed as described by Jang et al (13) with 2 L of PBS in the vortex chamber and the vacuum-cleaner running with a flow-rate of 1.5 m 3 /min.…”

Section: Description Of Sampling Methodsmentioning

confidence: 99%

Airborne Spread of Methicillin Resistant Staphylococcus aureus From a Swine Farm

et al. 2021

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Spread of livestock-associated methicillin resistant Staphylococcus aureus (LA-MRSA) to farmworkers has been recognized as a risk when working in LA-MRSA positive stables, due to LA-MRSA being present on airborne dust particles. Based on this, airborne spread of LA-MRSA through stable vents is a concern that is addressed in this study. The aim of the investigation was to quantify the airborne spread of LA-MRSA from a MRSA positive swine farm. In order to achieve this, a method for sampling large volumes of air was applied. The results were compared to meteorological data and bacteriological investigation of samples from the air inside the swine barn, soil outside the farm, and nasal samples from the individuals participating in the sampling process. MRSA was detected up to 300 m (the maximal measuring distance) from the swine farm in the air but only at low levels at distances above 50 meters (0.085 CFU/m3 at a distance of 50 m in the wind plume). MRSA was detected in sock samples obtained at the soil surfaces up to 400 m (the maximal measuring distance) from the farm building. The proportion of MRSA positive soil samples decreased from ~80 to 30% with increasing distance from the farm. A total of 25 human nasal samples were sampled after the farm visits after the participants had stayed in the surroundings of the farm for an average of 10.5 h. When leaving the farm, only two of the samples (8%) were LA-MRSA-positive both obtained from one individual who was the one who had sampled the ventilation shafts. In conclusion, airborne spread of MRSA from swine farms does not seem to be an important route for human contamination for individuals staying a whole working day outside a swine farm.

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“…A Cytosense flow cytometer (Cytobouy) was used to evaluate bacterial cell density, and its results were compared to quantitative PCR (qPCR). The regression analysis of this work showed that the evaluation of bacterial cell densities in both methods are almost the same, and the results proved that the proposed approach was most reliable for the estimation of bacterial cell densities in an outdoor environment [ 67 ].…”

Section: Bioaerosol Samplingmentioning

confidence: 98%

On-Site Bioaerosol Sampling and Airborne Microorganism Detection Technologies

Rastmanesh,

Boruah,

Lee

et al. 2024

Biosensors

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Bioaerosols are small airborne particles composed of microbiological fragments, including bacteria, viruses, fungi, pollens, and/or by-products of cells, which may be viable or non-viable wherever applicable. Exposure to these agents can cause a variety of health issues, such as allergic and infectious diseases, neurological disorders, and cancer. Therefore, detecting and identifying bioaerosols is crucial, and bioaerosol sampling is a key step in any bioaerosol investigation. This review provides an overview of the current bioaerosol sampling methods, both passive and active, as well as their applications and limitations for rapid on-site monitoring. The challenges and trends for detecting airborne microorganisms using molecular and immunological methods are also discussed, along with a summary and outlook for the development of prompt monitoring technologies.

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Application of Cytosense flow cytometer for the analysis of airborne bacteria collected by a high volume impingement sampler

Cited by 6 publications

References 45 publications

Biogenic Sources of Ice Nucleating Particles at the High Arctic Site Villum Research Station

Biogenic Sources of Ice Nucleating Particles at the High Arctic Site Villum Research Station

Airborne Spread of Methicillin Resistant Staphylococcus aureus From a Swine Farm

On-Site Bioaerosol Sampling and Airborne Microorganism Detection Technologies

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