Application of deglycosylation to SDS PAGE analysis improves calibration of influenza antigen standards

Harvey, Ruth; Hamill, Michelle; Robertson, James S.; Minor, Philip D.; Vodeiko, Galina M.; Weir, Jerry P.; Takahashi, Hitoshi; Harada, Yu; Itamura, Shigeyuki; Bamford, P.; Pozza, Tania Dalla; Engelhardt, Othmar G.

doi:10.1016/j.biologicals.2011.12.009

Cited by 19 publications

(22 citation statements)

References 7 publications

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“…The molecular composition (purity) and integrity of inactivated antigens should be established by appropriate biochemical methods and documented (Harvey et al 2012;Li et al 2014;Oxford et al 1981). Accurate quantification of the viral components is necessary for consistent results Pierce et al 2011;Williams et al 2008Williams et al , 2012.…”

Section: Laboratory Methods For Antigenic Characterizationmentioning

confidence: 99%

Antigenic Analyses of Highly Pathogenic Avian Influenza A Viruses

Donis

2014

Influenza Pathogenesis and Control - Volume I

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In response to the ongoing threat to animal and human health posed by HPAI endemic in poultry, Asia (H5N1) and North America (H7N3) have revived efforts to reduce pandemic risk by disease control at the source and improved pandemic vaccines. Discovery of conserved neutralization epitopes in the HA, which mediate broad protection within and across HA subtypes have changed the paradigm of "broadly reactive" or "universal" vaccine design. Development of such vaccines would benefit from comparative antigenic analysis of viruses with increasing divergence within (and between) HA subtypes. A review of recent work to define the antigenic properties of HPAI viruses revealed data generated through an array of experimental approaches. This information has supported diagnostics and vaccine development for animal and human health. Further harmonization of analytical methods is needed to determine the antigenic relationships among multiple lineages of rapidly evolving HPAI viruses.

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Section: Laboratory Methods For Antigenic Characterizationmentioning

confidence: 99%

Antigenic Analyses of Highly Pathogenic Avian Influenza A Viruses

Donis

2014

Influenza Pathogenesis and Control - Volume I

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“…These preparations are calibrated to estimate the HA content by a collaborative process of the four laboratories, which involves protein and quantitative SDS‐polyacrylamide gel electrophoresis (SDS‐PAGE) analyses. There have been significant recent improvements to reduce ambiguities in the SDS‐PAGE analysis by incorporation of an earlier deglycosylation step 21. Calibration of SRID reagents is described in a protocol endorsed by the WHO Expert Committee on Biological Standardization 22…”

Section: Key Features Of the Srid Assaymentioning

confidence: 99%

Standardisation of inactivated influenza vaccines—Learning from history

Wood

Weir

2018

Influenza Resp Viruses

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The single radial immunodiffusion assay has been the accepted method for determining the potency of inactivated influenza vaccines since 1978. The worldwide adoption of this assay for vaccine standardisation was facilitated through collaborative studies that demonstrated a high level of reproducibility and its applicability to the different types of influenza vaccine being produced at that time. Clinical evidence indicated the relevance of SRID as a potency assay. Unique features of the SRID assay are likely responsible for its longevity even as newer technologies for vaccine characterisation have been developed and refined. Nevertheless, there are significant limitations to the SRID assay that indicate the need for improvement, and there has been a substantial amount of work undertaken in recent years to develop and evaluate alternative potency assays, including collaborative studies involving research laboratories, regulatory agencies and vaccine manufacturers. Here, we provide an overview of the history of inactivated influenza vaccine potency testing, the current state of alternative assay development and the some of the major challenges to be overcome before implementation of new assays for potency determination.

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“…In addition, identity and purity of influenza vaccines are characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) [11][12][13]. Table 1 shows a comparison of selected properties of these three methods commonly used for the analysis of influenza vaccines.…”

Section: Introductionmentioning

confidence: 99%