Application of different colorimetric tests to cellodextrins

Miller, Gail Lorenz; Slater, Robert W.; Birzgalis, Rasma; Blum, Robert

doi:10.1016/0003-2697(61)90019-7

Cited by 12 publications

(6 citation statements)

References 10 publications

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“…All reagent preparations and procedures were done according to Miller et al [50] Reagents Dinitrosalicylic acid reagent (DNS reagent): dissolved by stirring 40 g dinitrosaliscylic acid, 8 g phenol and 2 g sodium sulphite in 100 mL. One percent of NaOH and 800 g Rochelle salt in 2 Lof 2% sodium hydroxide and then diluted to 4 L with distilled water.…”

Section: Dinitrosalicylic Acid Methodsmentioning

confidence: 99%

“…One percent of NaOH and 800 g Rochelle salt in 2 Lof 2% sodium hydroxide and then diluted to 4 L with distilled water. [50] Procedure The extract in test tubes was pipetted out from 0.5 to 3 mL and the volume was equalized at 3 mL with water in all the tubes. Three millilitres of DNS reagent was added, and the contents were heated in a boiling water bath for 10 min.…”

Section: Dinitrosalicylic Acid Methodsmentioning

confidence: 99%

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Biochemical studies on the production of biofuel (bioethanol) from potato peels wastes by Saccharomyces cerevisiae: effects of fermentation periods and nitrogen source concentration

Sheikh

Al-Bar

Ahmed

2016

Biotechnology & Biotechnological Equipment

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Section: Dinitrosalicylic Acid Methodsmentioning

confidence: 99%

Section: Dinitrosalicylic Acid Methodsmentioning

confidence: 99%

Biochemical studies on the production of biofuel (bioethanol) from potato peels wastes by Saccharomyces cerevisiae: effects of fermentation periods and nitrogen source concentration

Sheikh

Al-Bar

Ahmed

2016

Biotechnology & Biotechnological Equipment

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“…When the initial glucose was exhausted indicated by DNS (3,5‐dinitrosalicylic acid) method (Miller et al . ), the culture was starved for 0·5 h. Then, the induction phase proceeded and the threshold level of methanol, which can be utilized for growth and energy, was maintained around 1% with temperature shifted down from 30 to 25°C. The methanol content was measured by fuchsine sulfurous acid colorimetry (Wang et al .…”

Section: Methodsmentioning

confidence: 99%

Production and characterization of recombinant glucose oxidase from Aspergillus niger expressed in Pichia pastoris

Meng

Zhao

Yang

et al. 2014

Lett Appl Microbiol

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Significance and Impact of Study: Glucose oxidase is a very important enzyme produced by several species. However, large-scale applications have always been postponed by its complexity in fermentation and purification. Our research focused on developing new purification strategy of recombinant GOD from A. niger expressed in P. pastoris. Here, we described this novel one-step purification method and subsequent research in the characteristics of rGOD which showed different results from previous work. These can open new opportunities to increase its application. AbstractRecombinant glucose oxidase from Aspergillus niger expressed in Pichia pastoris by fed-batch fermentation was purified and assessed with 1Á26 purification fold to homogeneity using Q-Sepharose F.F. chromatography. The enzyme was determined by SDS-PAGE and gradient PAGE, which showed a dimeric form of 150 kDa. The purified rGOD was proved to be a glycoprotein, and the content of which was estimated to be 36Á7 and 25Á14% by phenol-sulfuric acid and anthrone-sulfuric acid methods. Characteristics demonstrated that the highest activity was in pH 6Á0 at 40°C and was stable at a broad pH range from 4Á0 to 9Á0 at 55°C or below. The optimum substrate for this enzyme was D-glucose, and the K m was 21Á06 mmol l À1 as well as the V max was 359 lmol min À1 mg À1 .rGOD possessed high resistance to various chemicals except for Hg 2+ , Fe 2+, Ag + , Cu 2+ , 1,4-dithiothreitol, sodium dodecyl sulfate and ascorbic acid. In addition, the inhibitors also exhibited intensive fluorescence quenching effect on rGOD.

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“…Spectrophotometric methods and an HPLC method for the determination of lactulose in lactulose syrups were compared by Parrish et al (1980). Total reducing sugars were measured by the 3,5-dinitrosalicylic acid method of Miller et al (1961), ketoses by the thiobarbituric acid method of Percheron (1962) and aldoses by the hypo-iodate oxidation method of Miller & Burton (1959). The methylamine method of Nickerson et al (1976) for lactose was found also to measure lactulose.…”

Section: Spectrophotometric Methodsmentioning

confidence: 99%