Application of different quantitative analytical techniques for estimation of aspirin and omeprazole in pharmaceutical preparation

Abstract

Several quantitative analytical methods were used to estimate aspirin and omeprazole in recently FDA-approved tablets. The first derivative of the ratio spectra was used to resolve the recorded overlapping spectra between aspirin and omeprazole. The first derivative of the ratio spectra of the studied drug mixtures was divided by a spectrum of a standard solution of omeprazole for the estimation of aspirin. Also, the first derivative of the ratio spectra of the studied drug mixtures was divided by a spectrum of the standard solution of aspirin for the estimation of omeprazole. For the simultaneous quantitative analysis of aspirin and omeprazole, the TLC densitometry technique was applied using TLC aluminum silica gel plates, toluene–acetonitrile–methanol (7:2:0.5, by volume) as the mobile phase, and UV detection at 272 nm. The advantages and disadvantages of the proposed techniques were discussed in the context of the results and the sensitivity limits of the methods. The proposed techniques were validated and successfully applied to the analysis of drugs in pure and pharmaceutical forms. A statistical comparison of the data obtained by the described methods with other data obtained by a previously published HPLC method was performed. The results agreed well with respect to the recommended statistical tests. Furthermore, the greenness of the described methods was assessed using different tools, the analytical eco-scale, the green analytical procedure index and the AGREE evaluation method. The proposed methods showed more adherence to the greenness characters in comparison to the previously reported HPLC method.

Development of validated methods for the simultaneous quantification of Finasteride and Tadalafil in newly launched FDA-approved therapeutic combination: greenness assessment using AGP, analytical eco-scale, and GAPI tools

“…As shown in Table 3, the results of the proposed methods were statistically compared with that of the reported one. 17 The values of t and F tests do not surpass the theoretical values, thus advocating that there is no signicant variation between the developed methods in the proposed study and the reported one. 3.5.…”

“…Greenness appraisal of the proposed methods in comparison with the reported one. Table 5 shows a comprehensive greenness behavior difference between the proposed methods and that of the reported one 17 for the quantication of FIN and TAD. Regarding the eco-scale applied tool, the eco-scale scores of the three methods were above 75, revealing the excellent green analytical method of analysis.…”

Green adherent spectrophotometric determination of molnupiravir based on computational calculations; application to a recently FDA-approved pharmaceutical dosage form

“…Computational chemistry represents an integral approach, in HPLC [4] , [5] , spectrophotometry [6] , [7] and electrochemistry [8] , to the development of greener methods based on theoretical testing of compound interactions and reducing the use of toxic solvents [9] . Many analytical methods have been developed to demonstrate the quality of the drug materials including HPLC [10] , TLC [11] , specroflorimetry [12] , [13] , [14] , [15] , [16] , [17] as well as spectrophotometry [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] , [28] . Table 1.…”

Green fitted second derivative synchronous spectrofluorometric method for simultaneous determination of remdesivir and apixaban at Nano gram scale in the spiked human plasma

“…In particular, the determination of drugs down to the Nano- gram range represents a potential approach for determination of drugs in biological fluids [1] , [2] , [3] . In addition, analytical chemists have recently devoted themselves to making analytical methods more environmentally friendly and safer for humans by using solvents and chemicals with low environmental impact, low energy consumption, and lower waste generation [4] , [5] , [6] , [7] . Analytical methods based on fluorescence spectroscopy provide a more sensitive tool for determining compounds of interest that exhibit unique fluorescence features [8] , [9] , [10] .…”