Application of diffusion tensor imaging in quantitatively monitoring chronic constriction injury of rabbit sciatic nerves: correlation with histological and functional changes

Wu, Wenjun; Niu, Yanfeng; Kong, Xiangquan; Dingxi, Liu; Liu, Xi; Shu, Shenglei; Su, Xiaoyun; Wang, Bing; Liu, Xiaoming; Ma, Yamei; Wang, Lixia

doi:10.1259/bjr.20170414

Cited by 10 publications

(9 citation statements)

References 30 publications

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Section: Discussioncontrasting

confidence: 51%

“…Experiments of constriction injury in rabbit sciatic nerves have reported microstructural alterations consistent with decreased FA and increased MD and RD (39,40). However, we observed increased AD while these authors cited significant (40) and non-significant (39) decreases in AD. In constriction injury, it appears that the chronic compression primarily restricts water motion parallel with the nerve and secondarily drives an inflammatory response of axonal swelling and loosening of the myelin sheath.…”

Section: Discussioncontrasting

confidence: 51%

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Peripheral Nerve Focused Ultrasound Lesioning—Visualization and Assessment Using Diffusion Weighted Imaging

et al. 2021

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Objectives: Magnetic resonance-guided focused ultrasound (MRgFUS) is a non-invasive targeted tissue ablation technique that can be applied to the nervous system. Diffusion weighted imaging (DWI) can visualize and evaluate nervous system microstructure. Tractography algorithms can reconstruct fiber bundles which can be used for treatment navigation and diffusion tensor imaging (DTI) metrics permit the quantitative assessment of nerve microstructure in vivo. There is a need for imaging tools to aid in the visualization and quantitative assessment of treatment-related nerve changes in MRgFUS. We present a method of peripheral nerve tract reconstruction and use DTI metrics to evaluate the MRgFUS treatment effect.Materials and Methods: MRgFUS was applied bilaterally to the sciatic nerves in 6 piglets (12 nerves total). T1-weighted and diffusion images were acquired before and after treatment. Tensor-based and constrained spherical deconvolution (CSD) tractography algorithms were used to reconstruct the nerves. DTI metrics of fractional anisotropy (FA), and mean (MD), axial (AD), and radial diffusivities (RD) were measured to assess acute (<1–2 h) treatment effects. Temperature was measured in vivo via MR thermometry. Histological data was collected for lesion assessment.Results: The sciatic nerves were successfully reconstructed in all subjects. Tract disruption was observed after treatment using both CSD and tensor models. DTI metrics in the targeted nerve segments showed significantly decreased FA and increased MD, AD, and RD. Transducer output power was positively correlated with lesion volume and temperature and negatively correlated with MD, AD, and RD. No correlations were observed between FA and other measured parameters.Conclusions: DWI and tractography are effective tools for visualizing peripheral nerve segments for targeting in non-invasive surgical methods and for assessing the microstructural changes that occur following MRgFUS treatment.

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Section: Discussioncontrasting

confidence: 51%

Section: Discussioncontrasting

confidence: 51%

Peripheral Nerve Focused Ultrasound Lesioning—Visualization and Assessment Using Diffusion Weighted Imaging

et al. 2021

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“…27 Numerous studies have demonstrated that decreased FA is a stable indicator for central nervous system injury such as demyelination. 28 Several mechanisms, such as reduction in axonal diameter, dendritic branching and fiber packing density or increases in myelination or microstructural loss of axon contribute to the greater FA, 29 so the increased FA does not indicate healthier white matter or beneficial results all the time. Meanwhile, the findings that the RD was significant increased in the bilateral superior longitudinal fasciculus indicating that there might be activity-dependent myelination in this brain region.…”

Section: Discussionmentioning

confidence: 99%

Brain Structure Alterations in Hemifacial Spasm: A Diffusion Tensor Imaging Study

Zhang¹,

Yu²,

et al. 2020

Clin EEG Neurosci

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This study examined white matter integrity in patients with left-sided hemifacial spasm (HFS) using diffusion tensor imaging (DTI). Thirty-six patients with left-sided HFS (mean age 53.24 ± 8.16 years) and 36 healthy volunteers (mean age 53.92 ± 7.73 years) were recruited. Tract-based spatial statistics (TBSS) analysis revealed significantly increased fractional anisotropy (FA) of bilateral superior longitudinal fasciculus in HFS patients ( P < 0.05, family-wise error corrected), with trends for radial diffusivity to decrease. We inferred that the results may be associated with poor sleep quality, impairment in visuospatial construction, and activity-dependent increases in myelination in HFS patients. Furthermore, the FA value of left superior longitudinal fasciculus showed a positive correlation with HFS duration ( r = 0.352, P = .041) and spasm severity ( r = 0.416, P = .014). However, the alteration of medial diffusivity and axial diffusivity were not found in bilateral superior longitudinal fasciculus between groups. These findings suggest FA changes of superior longitudinal fasciculus reflected by TBSS analysis may provide valuable insights into the diagnosis of HFS.

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“…Compared with the rat, there have been a relatively limited number of publications using the rabbit as the species to test nerve injury models. Some authors have addressed the "crush-injury" model [10][11][12][13][14][15][16][17][18][19] and not the transection model. Some authors differentiated between the tibial [20][21][22] and the peroneal [23][24][25][26][27] branches of the sciatic nerve as the site of injury for their model.…”

Section: Introductionmentioning

confidence: 99%

A sciatic nerve gap-injury model in the rabbit

Merolli

Voronin

et al. 2022

J Mater Sci: Mater Med

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There has been an increased number of studies of nerve transection injuries with the sciatic nerve gap-injury model in the rabbit in the past 2 years. We wanted to define in greater detail what is needed to test artificial nerve guides in a sciatic nerve gap-injury model in the rabbit. We hope that this will help investigators to fully exploit the robust translational potential of the rabbit sciatic nerve gap-injury model in its capacity to test devices whose diameter and length are in the range of those commonly applied in hand and wrist surgery (diameter ranging between 2 and 4 mm; length up to 30 mm). We suggest that the rabbit model should replace the less translational rat model in nerve regeneration research. The rabbit sciatic model, however, requires an effective strategy to prevent and control self-mutilation of the foot in the postoperative period, and to prevent pressure ulcers.

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Application of diffusion tensor imaging in quantitatively monitoring chronic constriction injury of rabbit sciatic nerves: correlation with histological and functional changes

Cited by 10 publications

References 30 publications

Peripheral Nerve Focused Ultrasound Lesioning—Visualization and Assessment Using Diffusion Weighted Imaging

Peripheral Nerve Focused Ultrasound Lesioning—Visualization and Assessment Using Diffusion Weighted Imaging

Brain Structure Alterations in Hemifacial Spasm: A Diffusion Tensor Imaging Study

A sciatic nerve gap-injury model in the rabbit

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