Application of dissolved oxygen (DO) level control for polyhydroxyalkanoate (PHA) accumulation with concurrent nitrification in surplus municipal activated sludge

Wang, Xiaofei; Bengtsson, Stina; Oehmen, Adrian; Carvalho, Gilda; Werker, Alan; Reis, Maria A.M.

doi:10.1016/j.nbt.2019.01.003

Cited by 27 publications

(10 citation statements)

References 30 publications

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“…Most of the studies have been focused on limiting nutrients like nitrogen, phosphorus to increase PHA yield in aerobic strains, but oxygen limitation and its impact on the survival of aerobic strains are rarely reported. Few studies were focused on how dissolved oxygen, and substrate competition was linked in enhancing PHA production (Wang et al, , 2017(Wang et al, , , 2019. This aspect need to be studied in detail to link the hypothesis that correlates decreased oxygen concentration on increased PHA yield (Kulpreecha et al, 2009;Mohammed et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Dissolved oxygen as a propulsive parameter for polyhydroxyalkanoate production using Bacillus endophyticus cultures

Madhusoodanan

Hariharapura

Divyashree

2021

Environ Dev Sustain

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Conventional biopolymers resembling synthetic polymers produced from microorganisms, polyhydroxyalkanoate (PHA) synthesized utilizing renewable resources have gained supreme attention recently. PHA accumulation within the microbial cell is an innate capability of bacteria to store carbon and energy when nutrient imbalance pertains. Gram positive Bacillus endophyticus capable of synthesizing PHA was focused in this study. Study focuses on the possibility of attaining high PHA yield in relation to the varying dissolved oxygen levels induced during production phase. There was a gradual increment in PHA production from 34.5 to 53.03% when cultivated in bioreactor that maintained least dissolved oxygen of 0.4 mg/L at 32 °C. The metabolic flux of organism was altered during oxygen stress brought by varying agitation rate and volume resulting in the accumulation of Nicotinamide Adenine Dinucleotide Hydrogen (NADH), which led to increase in the overall PHA production. PHA yield was found to be favored by decreasing the oxygen supply thereby inducing an oxygen stress environment. This report was the first one that was correlating the hypothesis that links PHA yield and oxygen stress condition during production phase. PHA produced was characterized by FTIR and 1HNMR spectra in which the presence of Polyhydroxybutyrate was confirmed. Graphical abstract

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Section: Introductionmentioning

confidence: 99%

Dissolved oxygen as a propulsive parameter for polyhydroxyalkanoate production using Bacillus endophyticus cultures

Madhusoodanan

Hariharapura

Divyashree

2021

Environ Dev Sustain

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“…Ammonium oxidation bacteria (AOB) and nitrite oxidation bacteria (NOB) were not found in the microbial community which was in line with the results that the enriched culture did not possess the nitrification capacity (Figure 1b). Compared to the traditional aerobic-feast and anoxic-famine process, which was inclined to select nitrifiers (AOB and NOB), 47 the strategy we proposed maintained a high oxygen level (DO = 7.5 mg/L) and ammonia-limited conditions which directed the evolution of the mixed culture toward highly-enriched aerobic PHA-storing denitrifiers.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Polyhydroxyalkanoate Production from Food Waste Coupled with Aerobic Nitrate Reduction

Liu

Xiong

et al. 2021

ACS Sustainable Chem. Eng.

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Polyhydroxyalkanoate (PHA) production from wastes by mixed microbial cultures has been developed into an integrated process to couple with nitrate removal due to its economic and environmental benefits. However, the anoxic conditions for denitrification result in low productivity of PHA production due to the limited energy supply. In this work, the fully aerobic feeding regime uncoupling carbon and nitrogen supply was applied to enhance the culture enrichment and productivity while being accompanied by nitrate removal. The enriched cultures showed a nitrate assimilation rate of 2.35 ± 1.10 mg of NO 3 − −N per gram of active biomass (X a ) per hour; meanwhile, the specific growth rate (μ X a , 0.019 ± 0.002 h −1 ) and biomass yield on PHA (Y X a /PHA , 0.68 ± 0.15 g X a /g PHA) were higher than those of a classic anoxic process (μ X a , 0.011 ± 0.001 h −1 and Y X a /PHA , 0.38 ± 0.09 g X a /g PHA). Furthermore, ammonia and oxygen were confirmed to have significant impacts on regulating nitrate utilization by directing nitrate flow to assimilatory nitrate use, aerobic denitrification, or anoxic denitrification. Metagenome analysis revealed that the overall function was mostly undertaken by Thauera aminoaromatica, and a nitrate metabolic model driven by PHAs was proposed. In summary, these findings widened application scenarios for PHA production coupled with nitrate removal.

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“…Different PHA copolymers were extracted from municipal activated sludge cultivated in fermented waste feedstock [11]. In biological wastewater treatment, nitrification occurs first where ammonia is converted to nitrate by ammonia and nitrite oxidizing bacteria (AOBs and NOBs) and finally it is removed as nitrogen via denitrification.…”

Section: Mixed Activated Sludge Processmentioning

confidence: 99%

“…However, sludge modification was proved to be fruitful in enhancing the PHA production. Several scientists conducted PHA extraction from different activated sludge like secondary municipal wastewater sludge, dissolved oxygen (DO) controlled municipal sludge, chemically treated and aerobic granular sludge [4,11,12].…”

Section: Introductionmentioning

confidence: 99%

Recovery of Biodegradable Bioplastics from Different Activated Sludge Processes during Wastewater Treatment

Ghosh

2021

Degradation of Plastics

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To overcome the environmental hazards of petroleum based plastics, synthesis and use of microbial bioplastics became popular. Polyhydroxyalkanoates (PHAs) are biodegradable biopolymers having plastic like properties mainly used in tissue engineering and packaging. Bacteria can produce bioplastics in carbon abundance. Activated sludge process is a simultaneous process for treating wastewater and producing PHAs. Wastewaters are treated by using mixed sludge, aerobic granular sludge and chemically treated sludge which provided more than 40% PHA yield. This chapter describes the PHA structure, synthesis pathways, types of wastewaters and activated sludge processes used with reactor parameters and environmental factors effecting PHA productions.

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Application of dissolved oxygen (DO) level control for polyhydroxyalkanoate (PHA) accumulation with concurrent nitrification in surplus municipal activated sludge

Abstract: Application of dissolved oxygen (DO) level control for polyhydroxyalkanoate (PHA) accumulation with concurrent nitrification in surplus municipal activated sludge,

Cited by 27 publications

References 30 publications

Dissolved oxygen as a propulsive parameter for polyhydroxyalkanoate production using Bacillus endophyticus cultures

Dissolved oxygen as a propulsive parameter for polyhydroxyalkanoate production using Bacillus endophyticus cultures

Polyhydroxyalkanoate Production from Food Waste Coupled with Aerobic Nitrate Reduction

Recovery of Biodegradable Bioplastics from Different Activated Sludge Processes during Wastewater Treatment

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