Application of Electron Paramagnetic Resonance and Solid-state 13C Nuclear Magnetic Resonance of Cross-polarization/Magic Angle Spinning to Study Enzymatic Degradation of Silk Fabrics

Zeng, Yuan; Peng, Ziwen; Wang, Bing; Hu, Zhiwen; Wan, Junmin; Zhou, Yang

doi:10.2116/analsci.33.579

Cited by 2 publications

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“…Compared with the proteomic analysis results of modern silk, the silk protein in these two cultural relic samples was in the final stage of degradation, and the heavy chain, light chain, and P25 chain were all degraded . Thus, only a small number of polypeptide molecules were retained, and they were adsorbed by soil particles. − …”

Section: Resultsmentioning

confidence: 88%

Rapid Enrichment and Detection of Silk Residues from Tombs by Double-Antibody Sandwich ELISA Based on Immunomagnetic Beads

et al. 2021

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The extraction and identification of silk residues in tombs is of great significance for studying the distribution and spread of early silk. However, the complex organic matter in the tomb hinders the accurate identification of silk. In this study, a doubleantibody sandwich enzyme-linked immunosorbent assay (ELISA) based on immunomagnetic beads (IMBs) was developed for the rapid enrichment and detection of silk residues. The doubleantibody sandwich ELISA method established by pairing the IMBs prepared by the silk fibroin monoclonal antibody SF-3 and the silk fibroin monoclonal-labeled antibody bio-SF-1 had the highest detection sensitivity, with a linear detection range of 10 to 10 4 ng mL −1 and a detection limit of 5.12 ng mL −1 . This method was excellent in the extraction and analysis of silk residues from archaeological imprints and soil samples and successfully identified silk residues in samples at the final stage of silk degradation (physical invisible silk). The proteomics analysis results demonstrated the feasibility and practicability of this method.

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Section: Resultsmentioning

confidence: 88%

Rapid Enrichment and Detection of Silk Residues from Tombs by Double-Antibody Sandwich ELISA Based on Immunomagnetic Beads

et al. 2021

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“…It is, therefore, necessary to understand the environmental degradation mechanisms that affect silk through its aging and to find reliable analytical markers for monitoring the degree of degradation of silk. Zeng et al investigated the enzymatic degradation mechanism of silk by combining various characterization methods, such as scanning electron microscopy (SEM), Fourier transfer infrared spectroscopy (FTIR), solid-state 13 C nuclear magnetic resonance of cross-polarization/magic angle spinning ( 13 C CP/MAS solid-state NMR), and electron spin resonance (ESR) [12]. Chen et al also explored the degradation process of silk using immunological detection techniques [13].…”

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confidence: 99%

Analytical insights for silk science and technology

Adachi

2023

ANAL. SCI.

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Caravans of camels carrying silk traveled westward across the endless desert. The exchange land route that stretches from China through Central Asia to Europe was called the "SILK ROAD." Sericulture, the process of raising silkworms and extracting silk thread, is understood to have appeared in China in the third millennium BC [1]. In the following millennia, silk was a significant cause of the magnificent and brilliant Chinese culture in the millennia following, and it facilitated contact between Eastern and Western civilizations via the "SILK ROAD." Currently, the world production of silk is about 110,000 tons per year [2]. This highlight is expected to provide fundamental analytical insights into silk fibroin's chemical structure and inspire various researchers to analyze historical or novel silk materials considering silk's characteristic chemical and physical properties. Spider silk threads [3,4] and bagworm silk threads [5] will be introduced at another opportunity.Most silk-producing silkworms (wild silkworms, [e.g., Samia cynthia ricini (eri silkworm), Antheraea mylitta (tasar silkworm), Antheraea pernyi (temperate tasar silkworm), Antheraea mylitta Drury (tropical tasar silkworm), Antheraea assama (muga silkworm), Caligzlla japonica (Japanese chestnut silkworm), Antheraea yamamai (Japanese oak silkworm)]) can feed on a wide variety of leaves, but Bombyx mori (mulberry silkworm; domestic silkworm) grow by eating only mulberry leaves. The ingested leaf pieces are digested and absorbed as amino acids in the digestive tract. The liquid silk is then synthesized from various amino acids in the silk gland. The resulting liquid silk is spun as an insoluble single cocoon filament through the spinneret on the head of the caterpillar. Mulberry silkworms produce

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Application of Electron Paramagnetic Resonance and Solid-state 13C Nuclear Magnetic Resonance of Cross-polarization/Magic Angle Spinning to Study Enzymatic Degradation of Silk Fabrics

Cited by 2 publications

References 26 publications

Rapid Enrichment and Detection of Silk Residues from Tombs by Double-Antibody Sandwich ELISA Based on Immunomagnetic Beads

Rapid Enrichment and Detection of Silk Residues from Tombs by Double-Antibody Sandwich ELISA Based on Immunomagnetic Beads

Analytical insights for silk science and technology

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