Application of experimental design and response surface technique for selecting the optimum RP-HPLC conditions for the determination of moxifloxacin HCl and ketorolac tromethamine in eye drops

Kalariya, Pradipbhai D.; Namdev, Deepak; Srinivas, R.; Gananadhamu, S.

doi:10.1016/j.jscs.2014.04.004

Cited by 29 publications

(22 citation statements)

References 23 publications

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“…MOXI may be analysed by many analytical techniques. The predominant ones are reversed phase HPLC with different detectors such as UV [ 20 , 24 , 54 , 60 , 74 , 75 ], FLD [ 27 , 29 , 76 – 79 ], MS [ 80 – 82 ], and DAD [ 57 , 66 ]. There are also reported methods for CE [ 32 , 83 , 84 ].…”

Section: Fourth Generation Fluoroquinolonesmentioning

confidence: 99%

“…The main constituent of the mobile phase in HPLC separation was the phosphate buffer [ 24 , 29 , 54 , 66 , 74 – 76 , 78 ]. The concentration of the phosphate buffer was within the range 10–50 mM; however, there is also a reported method with a high concentration of sodium phosphate (0.25 M) [ 78 ].…”

Section: Fourth Generation Fluoroquinolonesmentioning

confidence: 99%

“…In many methods an ion pair reagent was the constituent of the mobile phase. The most common used was TEA [ 60 , 66 , 74 , 75 , 80 ]. The concentration was in the range 0.03–2.00%.…”

Section: Fourth Generation Fluoroquinolonesmentioning

confidence: 99%

“…The decrease of pH value to 3.5 results in reducing of peaks tailing. The silanol groups above pH 3.5 are ionized and interact with 1° and 2° amines [ 74 ]. The content of the water-based phase in isocratic elution was 57–95%.…”

Section: Fourth Generation Fluoroquinolonesmentioning

confidence: 99%

“…The content of the water-based phase in isocratic elution was 57–95%. The organic solvents applied in the chromatographic analysis were ACN [ 20 , 24 , 29 , 54 , 57 , 60 , 76 , 78 , 79 , 81 , 82 ], methanol [ 66 , 74 , 80 ], or both [ 27 , 75 , 77 ]. The pH of the mobile phase was acidic 2.5–6.0.…”

Section: Fourth Generation Fluoroquinolonesmentioning

confidence: 99%

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Analytical Methods for Determining Third and Fourth Generation Fluoroquinolones: A Review

Czyrski

2016

Chromatographia

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Fluoroquinolones of the third and fourth generation posses wide bactericidal activity. Monitoring concentrations of antibacterial agents provides effective therapy and prevents the increase of bacterial resistance to antibiotics. The pharmacodynamic parameters that best describe fluoroquinalone activity are AUC/MIC and C max/MIC. Determining the level of this type of drug is essential to reach the effective concentration that inhibits the growth of bacteria. Determining the pharmaceutical formulation confirms the purity of a substance. Many methods have been developed to determine the level of these substances. They involve mainly the following analytical techniques: chromatography, capillary electrophoresis, and spectroscopy. The separation techniques were combined with different measuring devices, such as ultraviolet (UV), fluorescence detector (FLD), diode array detector (DAD), and mass spectrometry (MS). The analytical procedures require proper sample pre-conditioning such as protein precipitation, extraction techniques, filtration, or dilution. This paper reviews the reported analytical methods for the determining representatives of the third and fourth generation of fluoroquinolones. Attention was paid to pre-conditioning of the samples and the applied mobile phase. This report might be helpful in the selection of the proper procedure in determining the abovementioned drugs in different matrices.Graphical abstract

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Section: Fourth Generation Fluoroquinolonesmentioning

confidence: 99%

Section: Fourth Generation Fluoroquinolonesmentioning

confidence: 99%

“…In many methods an ion pair reagent was the constituent of the mobile phase. The most common used was TEA [ 60 , 66 , 74 , 75 , 80 ]. The concentration was in the range 0.03–2.00%.…”

Section: Fourth Generation Fluoroquinolonesmentioning

confidence: 99%

Section: Fourth Generation Fluoroquinolonesmentioning

confidence: 99%