Application of Factorial Design Experiments to the Development of a Disposable Amperometric DNA Biosensor

Uliana, Carolina Venturini; Tognolli, João Olímpio; Yamanaka, Hideko

doi:10.1002/elan.201100269

Cited by 23 publications

(10 citation statements)

References 29 publications

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“…To date, different types of biosensors have been developed including electrochemical, piezoelectric, optical, surface plasmon resonance, quartz crystal microbalance, fluorescence, magnetic etc. ( Adam et al, 2010 ; Luo et al, 2008 ; Uliana et al, 2011 ; Xue et al, 2007 ; Zhu et al, 2009 ). In a biosensor, the target analyte of interest interacts with the pre-designed bioreceptor and produces a signal for the transducer.…”

Section: Principle Of Biosensormentioning

confidence: 99%

Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: State of the art

Khan¹,

Hasan

Hossain

et al. 2020

Biosensors and Bioelectronics

174

108

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Last few decades, viruses are a real menace to human safety. Therefore, the rapid identification of viruses should be one of the best ways to prevent an outbreak and important implications for medical healthcare. The recent outbreak of coronavirus disease (COVID-19) is an infectious disease caused by a newly discovered coronavirus which belongs to the single-stranded, positive-strand RNA viruses. The pandemic dimension spread of COVID-19 poses a severe threat to the health and lives of seven billion people worldwide. There is a growing urgency worldwide to establish a point-of-care device for the rapid detection of COVID-19 to prevent subsequent secondary spread. Therefore, the need for sensitive, selective, and rapid diagnostic devices plays a vital role in selecting appropriate treatments and to prevent the epidemics. During the last decade, electrochemical biosensors have emerged as reliable analytical devices and represent a new promising tool for the detection of different pathogenic viruses. This review summarizes the state of the art of different virus detection with currently available electrochemical detection methods. Moreover, this review discusses different fabrication techniques, detection principles, and applications of various virus biosensors. Future research also looks at the use of electrochemical biosensors regarding a potential detection kit for the rapid identification of the COVID-19.

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Section: Principle Of Biosensormentioning

confidence: 99%

Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: State of the art

Khan¹,

Hasan

Hossain

et al. 2020

Biosensors and Bioelectronics

174

108

View full text Add to dashboard Cite

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“…Two major features of electrochemical bioreceptors are modifiable probes or antibodies followed by target isolation, and amplified signal detection (Figure 6 ) 128 . The amplified signals can be either quantitative potentinmetric, amperometric, or impedimetric; and the signal can be either labeled or label-free, although labels are often required because electrochemical signals are usually less sensitive 88 . Other features such as hybridization, and amplified or changed signal intensity can be applied to electrochemical biosensors.…”

Section: Biosensors In Poc Of Foodborne Virusesmentioning

confidence: 99%

Recent Advances in Biosensor Development for Foodborne Virus Detection

Neethirajan¹,

Ahmed²,

Chand³

et al. 2017

Nanotheranostics

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Outbreaks of foodborne diseases related to fresh produce have been increasing in North America and Europe. Viral foodborne pathogens are poorly understood, suffering from insufficient awareness and surveillance due to the limits on knowledge, availability, and costs of related technologies and devices. Current foodborne viruses are emphasized and newly emerging foodborne viruses are beginning to attract interest. To face current challenges regarding foodborne pathogens, a point-of-care (POC) concept has been introduced to food testing technology and device. POC device development involves technologies such as microfluidics, nanomaterials, biosensors and other advanced techniques. These advanced technologies, together with the challenges in developing foodborne virus detection assays and devices, are described and analysed in this critical review. Advanced technologies provide a path forward for foodborne virus detection, but more research and development will be needed to provide the level of manufacturing capacity required.

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“…The first step in the development of an impedimetric immunosensor (Scheme 1-1) is the addition of 10 µL of 1 × 10 −3 mol·L −1 lipoic acid prepared in ethanol/water solution (1:10) to the gold CD-trode surface and incubation for 2 h to form a SAM [31]. According to literature, both thiol and disulfide groups interact with gold [11].…”

Section: Immunosensor Developmentmentioning

confidence: 99%

A Low-Cost Label-Free AFB1 Impedimetric Immunosensor Based on Functionalized CD-Trodes

et al. 2016

Self Cite

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This work describes the investigation of a label-free immunosensor for the detection of aflatoxin B 1 (AFB 1 ). CD-trodes (electrodes obtained from recordable compact disks) were used as low-cost and disposable transducers after modification with a self-assembled monolayer (SAM) of lipoic acid. The anti-aflatoxin B 1 antibody was immobilized via EDC/NHS activation, followed by blocking with bovine serum albumin and immunoassays with AFB 1 . The optimization of analytical parameters and the detection were carried out using electrochemical impedance measurements. Using chemometric tools, the best conditions for the immunosensor development were defined as: anti-AFB 1 antibody at 1:2000 dilution and surface blocking with 0.5% bovine serum albumin, both incubated for 1 h, and antibody-antigen immunoreaction for 30 min. The impedimetric immunosensor showed a linear range from 5 × 10 −9 to 1 × 10 −7 mol·L −1 (1.56-31.2 ng·mL −1 ), limit of detection and limit of quantification, respectively, 3.6 × 10 −10 and 1.1 × 10 −9 mol·L −1 (0.11 and 0.34 ng·mL −1 ). The proposed immunosensor was applied to analyze peanut samples.

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Application of Factorial Design Experiments to the Development of a Disposable Amperometric DNA Biosensor

Cited by 23 publications

References 29 publications

Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: State of the art

Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: State of the art

Recent Advances in Biosensor Development for Foodborne Virus Detection

A Low-Cost Label-Free AFB1 Impedimetric Immunosensor Based on Functionalized CD-Trodes

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