Application of flow injection?chemiluminescence to the study of radical scavenging activity in plants

Choi, Hong Yeob; Jhun, Eun Ja; Lim, Beong Ou; Chung, Ill Min; Kyung, Suk‐Hun; Park, Dong Ki

doi:10.1002/1099-1573(200006)14:4<250::aid-ptr587>3.0.co;2-j

Cited by 75 publications

(49 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To assess age-related phospholipid peroxidation, we used the flow injection-chemiluminescence (FI-CL) method that has been developed to monitor active oxy gen-derived radical activity (18). Previous reports from our laboratory described a simple and continuous one step flow injection system based on cytochrome c-am plified chemiluminescence for the assay of radical scav enging activity (18,19).…”

Section: Discussionmentioning

confidence: 94%

Dietary Restriction Downregulates Free Radical and Lipid Peroxide Production: Plausible Mechanism for Elongation of Life Span.

Lim

Sugano

2002

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

Self Cite

View full text Add to dashboard Cite

SummaryDietary restriction elongates life span by suppressing age-related diseases in experimental animals. It has received a great deal of attention in connection with the rela tionship between aging, nutrition, and oxidative stress because oxidative injury in several tissues is a prominent feature in the aging process. Although the oxidative stress theory of aging has currently gained popularity, the premise from which this hypothesis was derived is paradoxical because the same oxygen, that supports life in one hand threatens survival and promotes aging in the other. Until recently, no single experimental paradigm could offer satisfactory mechanistic explanations for this complex issue. Recent investigations using the life-extending dietary restriction regimen could offer satisfactory mechanistic explanations for this apparent self contradiction to life. The modulation of free radical-induced oxidative stress provided sufficient data to support the notion that dietary restriction's antiaging ef fect may come from its ability to tightly regulate the oxidative status of an organism. The re sult is the maintenance of cellular homeostasis, a hallmark of dietary restriction's action in the extension of life span. To date, we reported that dietary restriction (maintained on 60% of ad libitum feeding) suppresses age-related oxidative damage by modulating the amount as well as the fatty acid composition of tissue phospholipids. These remarkable findings have been incorporated into the new "membrane peorxidation cycle" concept. The intervention of this cycle appears to be an evolutionary process that the dietary restricted rats have adapted as a strategy to protect the membrane in an oxidative environment.

show abstract

Section: Discussionmentioning

confidence: 94%

Dietary Restriction Downregulates Free Radical and Lipid Peroxide Production: Plausible Mechanism for Elongation of Life Span.

Lim

Sugano

2002

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

Self Cite

View full text Add to dashboard Cite

show abstract

“…Subsequently, the percentage of inhibition was plotted against concentration, and from the graph the IC 50 was determined (16).…”

Section: Determination Of Total Antioxidant Capacity (Tac)mentioning

confidence: 97%

Anticancer activity and antioxidant potential of Aponogeton undulatus against Ehrlich ascites carcinoma cells in Swiss albino mice

et al. 2017

View full text Add to dashboard Cite

Abstract. The in vivo anticancer and in vitro antioxidant effects of the crude methanol extract of Aponogeton undulatus, in addition to its various organic fractions, were investigated. Various assay methods, including the total antioxidant capacity assay, lipid peroxidation inhibition assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay and ferrous reducing power assessment were used to evaluate the antioxidant potential of the crude extract and its organic fractions. The ethyl acetate fraction (EAU) demonstrated the highest antioxidant capacity (175.80±0.41 mg/g equivalent ascorbic acid), which exhibited IC 50 values of DPPH scavenging activity and maximum lipid peroxidation of 38.84±0.02 and 42.52±0.32 µg/ml, respectively. The reducing power of the extract was concentration dependent. The in vivo antitumor activity was evaluated against Ehrlich ascites carcinoma (EAC) cell-bearing Swiss albino mice. EAUs (50, 100 and 200 mg/kg body weight) were administered for nine consecutive days. On day 10, half of the mice were sacrificed and the remaining mice were evaluated for lifespan. Hematological profiles including, red blood cell, white blood cell and hemoglobin content were also evaluated. EAU treatment significantly (P<0.05) decreased tumor volume, packed cell volume and viable cell count, and increased the lifespan of EAC tumor-bearing mice. Hematological profiles were restored to normal levels in EAU-treated mice compared with EAC control mice. Taken together, these results suggest that the Aponogeton undulatus extract may have potent antioxidant and anticancer properties. IntroductionIn addition to numerous types of oxygen-centered free radicals, various physiological and biochemical processes in the human body may produce other reactive oxygen species (ROS) (1). High concentrations of these free radicals may induce oxidative damage to cell structures including lipids and membranes, proteins and nucleic acids; this damage is often referred to as oxidative stress and eventually leads to numerous chronic diseases, including atherosclerosis, cancer, diabetes and other degenerative diseases in humans (2,3). ROS have previously been revealed to be involved in cancer initiation and promotion, and patients with neoplasms demonstrated elevated malondialdehyde (MDA) concentrations (4). Furthermore, evidence indicating that antioxidants inhibit free radical damage suggests that treatment with a combination of antioxidants may be a potent adjunctive preventive treatment for cancer (5).Phytochemicals are still widely used worldwide and across the major groups of human medicine, presently, they comprise ~50% of the total pharmaceutical market (6). A number of countries and studies are extracting and investigating potent and nontoxic antioxidants from natural sources, in particular those identified in edible or medicinal plants, in order to prevent free radical-associated human disorders and to replace synthetic compounds, which likely possess carcinogenic activity or activity that is harmful to the l...

show abstract

“…The method as expressed by Choi et al, with slight alterations was used for the determination of DPPH radical scavenging activity [37].…”

Section: Dpph Radical Scavenging Assaymentioning

confidence: 99%

Analysis of in vitro antioxidant activity of Caryota urens L. leaves: A traditional natural remedy

Mamun¹,

Khanum²,

Begum³

et al. 2016

J Coast Life Med

View full text Add to dashboard Cite

Application of flow injection?chemiluminescence to the study of radical scavenging activity in plants

Cited by 75 publications

References 12 publications

Dietary Restriction Downregulates Free Radical and Lipid Peroxide Production: Plausible Mechanism for Elongation of Life Span.

Dietary Restriction Downregulates Free Radical and Lipid Peroxide Production: Plausible Mechanism for Elongation of Life Span.

Anticancer activity and antioxidant potential of Aponogeton undulatus against Ehrlich ascites carcinoma cells in Swiss albino mice

Analysis of in vitro antioxidant activity of Caryota urens L. leaves: A traditional natural remedy

Contact Info

Product

Resources

About