Application of Förster Resonance Energy Transfer (FRET) technique to elucidate intracellular and In Vivo biofate of nanomedicines

Chen, Tongkai; He, Bing; Tao, Jing-Song; He, Yuan; Deng, Hailiang; Wang, Xueqing; Zheng, Ying

doi:10.1016/j.addr.2019.04.009

Cited by 144 publications

(69 citation statements)

References 163 publications

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“…Metabolic cell‐labeling and ‐imaging by metabolic oligosaccharide engineering of unnatural carbohydrates may be effective in evaluating the cellular pharmacokinetics of NPs due to higher biocompatibility and specificity. [ 235 ] Alternatively, other environment‐responsive fluorescence probes based on aggregation‐caused quenching, [ 233,236 ] aggregation‐induced emission, [ 237 ] or förster resonance energy transfer [ 238 ] are a potential tool to quantify the NPs and accurately monitor their fate in cell models. In this article, we have explored the benefits of several intracellular delivery strategies as well as their limitations, particularly safety concerns and scalability.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Nanocarrier‐Mediated Cytosolic Delivery of Biopharmaceuticals

Xing

Wang

et al. 2020

Adv Funct Materials

125

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Biopharmaceuticals have emerged to play a vital role in disease treatment and have shown promise in the rapidly expanding pharmaceutical market due to their high specificity and potency. However, the delivery of these biologics is hindered by various physiological barriers, owing primarily to the poor cell membrane permeability, low stability, and increased size of biologic agents. Since many biological drugs are intended to function by interacting with intracellular targets, their delivery to intracellular targets is of high relevance. In this review, the authors summarize and discuss the use of nanocarriers for intracellular delivery of biopharmaceuticals via endosomal escape and, especially, the routes of direct cytosolic delivery by means including the caveolae‐mediated pathway, contact release, intermembrane transfer, membrane fusion, direct translocation, and membrane disruption. Strategies with high potential for translation are highlighted. Finally, the authors conclude with the clinical translation of promising carriers and future perspectives.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Nanocarrier‐Mediated Cytosolic Delivery of Biopharmaceuticals

Xing

Wang

et al. 2020

Adv Funct Materials

125

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“…Figures with diminished C6 staining as in HepG2 and MCF7 were mostly attributed to the affinity of PI to the DNA of dead cells. Once PI binds with the DNA, its fluorescence increases 20-30 times masking the fluorescence intensity of C6 due to the Förster resonance energy transfer phenomena 19 . Therefore, with more dead cells due to GA/C6NPs, it will be challenging to have the real background color of C6 nanoparticles.…”

Section: The Antihypertensive Activity Of Ganps the In Vitro Antihypmentioning

confidence: 99%

Preparation, characterization and therapeutic properties of gum arabic-stabilized gallic acid nanoparticles

Hassani

Azarian

Ibrahim

et al. 2020

Sci Rep

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Gallic acid (GA) is a natural phenolic compound with therapeutic effects that are often challenged by its rapid metabolism and clearance. Therefore, GA was encapsulated using gum arabic into nanoparticles to increase its bioavailability. The formulated nanoparticles (GANPs) were characterized for physicochemical properties and size and were then evaluated for antioxidant and antihypertensive effects using various established in vitro assays, including 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide scavenging (NO), β-carotene bleaching and angiotensin-converting enzyme (ACE) inhibitory assays. The GANPs were further evaluated for the in vitro cytotoxicity, cell uptake and cell migration in four types of human cancer cell lines including (MCF-7, MDA-MB231) breast adenocarcinoma, HepG2 hepatocellular cancer, HT-29 colorectal adenocarcinoma, and MCF-10A breast epithelial cell lines. The GANPs demonstrated potent antioxidant effects and have shown promising anti-cancer properties in a dose-dependent manner with a predilection toward HepG2 and MCF7 cancer cells. The uptake of GANPs was successful in the majority of cancer cells with a propensity to accumulate in the nuclear region of the cells. The HepG2 and MCF7 cancer cells also had a significantly higher percentage of apoptosis and were more sensitive to gallic acid nanoparticle treatment in the cell migration assay. This study is the first to confirm the synergistic effects of gum arabic in the encapsulation of gallic acid by increasing the selectivity towards cancer cells and enhancing the antioxidant properties. The formulated nanoparticles also had remarkably low toxicity in normal cells. Based on these findings, GANPs may have promising therapeutic applications towards the development of more effective treatments with a probable targeting precision in cancer cells.

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“…The experimental validation of the predicted MP-MP interactions could be tested with techniques such as FRET (104), or by our exclusive Microtubule Bench approach (105). By applying machine learning methods, the PPIMem method can be improved by insuring that the MPs belong to the same developmental stage, tissue, cell type, site of expression, reaction and metabolic pathways, display functional similarity, and do not show a gene distance of more than 20 (106).…”

Section: Discussionmentioning

confidence: 99%

Getting to know each other: PPIMem, a novel approach for predicting transmembrane protein-protein complexes

Khazen

Gyulkhandanian

Issa

et al. 2019

Preprint

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Membrane proteins account for about one-third of the proteomes of organisms and include structural proteins, channels, and receptors. The mutual interactions they establish in the membrane play crucial roles in organisms, as they are behind many processes such as cell signaling and protein function. Because of their number and diversity, these proteins and their macromolecular complexes, along with their physical interfaces represent potential pharmacological targets par excellence for a variety of diseases, with very important implications for the design and discovery of new drugs or peptides modulating or inhibiting the interaction. Yet, structural data coming from experiments is very scarce for this family of proteins. To overcome this problem, we propose a computational approach for the prediction of alpha transmembrane protein multimeric higher-order structures through data mining, sequence analysis, motif search, extraction, identification and characterization of the amino acid residues at the interface of the complexes. This method leads us to the formulation of binding sites used to scan protein sequence datasets for generating new potential interacting protein couples. Our template motif-based approach using experimental recognition sites leads us to predict new binding sites and to thousands of new binary complexes between membrane proteins when allowing amino acid mutations to take place. We generate an online database of the annotated predicted interactions.

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Application of Förster Resonance Energy Transfer (FRET) technique to elucidate intracellular and In Vivo biofate of nanomedicines

Cited by 144 publications

References 163 publications

Nanocarrier‐Mediated Cytosolic Delivery of Biopharmaceuticals

Nanocarrier‐Mediated Cytosolic Delivery of Biopharmaceuticals

Preparation, characterization and therapeutic properties of gum arabic-stabilized gallic acid nanoparticles

Getting to know each other: PPIMem, a novel approach for predicting transmembrane protein-protein complexes

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