Application of Genomic and Proteomic Data for Bioprocess Analysis and Optimization

Zeng, An‐Ping; Sun, Jibin; Wangb, Wei; Maa, Hongwu; Deckwerb, Wolf-Dieter

doi:10.1016/b978-044452114-9/50003-7

Cited by 1 publication

(2 citation statements)

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“…1). The concentration range for synthetic 1,3-PD solution was determined to be similar to the concentration of 1,3-PD produced by the fermentation according to literature [4][5][6][7][8][9].…”

Section: Rsmmentioning

confidence: 99%

“…The final concentration of 1,3-PD in typical glycerol fermentations is limited due to product inhibition, ranging from 50 to 85 g/L [4][5][6][7][8][9]. In some recent studies, 1,3-PD final concentration beyond this level has been obtained with recombinant strains or under unusual fermentation conditions such as the use of high yeast extract concentration in the medium.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Selection and optimization of an aqueous two‐phase system for the recovery of 1,3‐propandiol from fermentation broth

Aydoğan¹,

Bayraktar²,

Mehmetoǧlu³

et al. 2010

Engineering in Life Sciences

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In this study a suitable alcohol/salt aqueous two‐phase (ATP) system was selected for the recovery of 1,3‐propandiol (1,3‐PD) from fermentation broth. From the different alcohol/salt systems studied the ethanol and dipotassium hydrogen phosphate ATP system appeared to be favorable. To examine the potential of this ATP system the partition coefficient of 1,3‐PD in synthetic solutions was first optimized with the response surface methodology. The parameters studied were concentrations of ethanol (21.99–38.81% w/w), dipotassium hydrogen phosphate (14.99–31.81% w/w) and 1,3‐PD (6.36–73.64 g/L). The optimum conditions were found to be 35.39% w/w for ethanol, 28.40% w/w for dipotassium hydrogen phosphate and 73.6 g/L for 1,3‐PD. Under these conditions the maximum partition coefficient of 1,3‐PD and the extraction yield were determined as 23.14 and 97.82%, respectively. The optimum extraction conditions were then used to guide the recovery of 1,3‐PD from a real fermentation broth. The partition coefficient and extraction yield of 1,3‐PD reached 20.28–97.20% in this case, respectively. A favorable partition of the organic acids lactate, acetate and butyrate in the bottom phase was also achieved. We have also studied the removal of cells and macromolecules from the broth. Removal ratio of cells and proteins were 96.47 and 93.05%, respectively. Thus, the ethanol/dipotassium hydrogen phosphate ATP system appears to be an interesting alternative or can be used as one useful step in the downstream processing of 1,3‐PD from fermentation broth.

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