Selection and optimization of an aqueous two‐phase system for the recovery of 1,3‐propandiol from fermentation broth

Abstract

In this study a suitable alcohol/salt aqueous two‐phase (ATP) system was selected for the recovery of 1,3‐propandiol (1,3‐PD) from fermentation broth. From the different alcohol/salt systems studied the ethanol and dipotassium hydrogen phosphate ATP system appeared to be favorable. To examine the potential of this ATP system the partition coefficient of 1,3‐PD in synthetic solutions was first optimized with the response surface methodology. The parameters studied were concentrations of ethanol (21.99–38.81% w/w), dipotassium hydrogen phosphate (14.99–31.81% w/w) and 1,3‐PD (6.36–73.64 g/L). The optimum conditions were found to be 35.39% w/w for ethanol, 28.40% w/w for dipotassium hydrogen phosphate and 73.6 g/L for 1,3‐PD. Under these conditions the maximum partition coefficient of 1,3‐PD and the extraction yield were determined as 23.14 and 97.82%, respectively. The optimum extraction conditions were then used to guide the recovery of 1,3‐PD from a real fermentation broth. The partition coefficient and extraction yield of 1,3‐PD reached 20.28–97.20% in this case, respectively. A favorable partition of the organic acids lactate, acetate and butyrate in the bottom phase was also achieved. We have also studied the removal of cells and macromolecules from the broth. Removal ratio of cells and proteins were 96.47 and 93.05%, respectively. Thus, the ethanol/dipotassium hydrogen phosphate ATP system appears to be an interesting alternative or can be used as one useful step in the downstream processing of 1,3‐PD from fermentation broth.

Aqueous two-phase system: An alternative process for recovery of succinic acid from fermentation broth

“…Coomassie Brilliant Blue G250 was purchased from the ShanghaiSangon Biotech Co., Ltd. (Shanghai, China). The fermentation broth was obtained from glucose-based fed-batch fermentation by A. succinogenes CGMCC1593 in a 5-L stirred fermentor (BIOFLO 110, the New Brunswick Scientific) under anaerobic conditions as previously described [39,40]. The fermentation medium contained the following (per L): glucose, 50 g; corn steep liquor, 25 g; K 2 HPO 4 3H 2 O, 2.5 g; NaH 2 PO 4 2H 2 O, 2.5 g; MgCl 2 , 0.2 g; CaCl 2 , 0.2 g; Vitamin B12, 20 lg; Vitamin B6, 50 lg; riboflavin, 50 lg; lipoic acid, 50 lg; niacin, 50 lg; thiamine, 50 lg; folic acid, 50 lg; pantothenate, 50 lg; p-aminobenzoate, 50 lg; and biotin, 100 lg.…”

“…Some studies have reported that this method has the advantages of versatility, high efficiency, high yield, improved purification factor, selectivity, low cost, and fast mass transfer rates [31]. Recently, when compared with the conventional ATPS made of dual polymer systems or polymer/salt systems, a novel ATPS, comprising hydrophilic organic solvent such as short-chain alcohols and inorganic salt, has been successfully used in the extraction of bulk chemicals [35], such as 2,3-butanediol and 1,3-propanediol [36][37][38][39][40][41], from the fermentation broth. The novel system uses cheaper extractants instead of expensive polymers, and has the advantages of low interfacial tension, high resolution, and easy recovery of hydrophilic organic solvent through evaporation.…”

Different types of aqueous two‐phase systems for biomolecule and bioparticle extraction and purification

“…Another type of ATPS exists, but it is not discussed in this manuscript ATPS, formed by short‐chain alcohols and inorganic salts. This type of system was used for the extraction of serine protease, antibiotics of tetracycline hydrochloride, an active ingredient from natural plant of lithospermic acid B, natural pigment of crocins, salvianolic acid B, glycyrrhizin, and lipase . Some description of different system formed by these components can be found in Greve and Kula, 1991 …”

High tolerance to glycerol and high production of 1,3‐propanediol in batch fermentations by microbial consortium from marine sludge

“…Klebsiella pneumoniae or Clostridium butyricum . In pure cultures of single strains, many by‐products would be generated, such as alcohols and organic acids, leading to low conversion rate of substrate and much difficulty in the separation and purification of 1,3‐PD . For example, 2,3‐butandiol (2,3‐BD) as a by‐product is similar to the target product, 1,3‐PD, in the fermentation broth by K. pneumoniae .…”