Application of Glyceroglycolipids, Photosynthetic Pigments and Extracts of Brown Algae for Suppression ROS

Abstract

Lipid-rich extracts were obtained from brown algae Saccharina cichorioides, Costaria costata, Chorda filum, Eularia fistulosa, Dictyopteris divaricata, Dictyosiphon chordaria, Silvetia babingtonii, and Fucus evanescens that were collected in Peter the Great Gulf of the Sea of Japan. The ability of algal extracts and glyceroglycolipids (GLs) monogalactosyldiacylglycerols (MGDG), digalactosyldiacylglycerols (DGDG), and sulfoquinovosyldiacylglycerols (SQDG) and carotenoid fucoxanthin to suppression of reactive oxygen species (ROS) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells, studied.The results showed that algae extracts could suppress ROS. However, extracts of D. divaricata, D. chordaria, C. filum, S. babingtonii, and F. evanescens had a higher degree of suppression of ROS. Extracts of S. cichorioides and D. divaricata showed the dependence of their activity from the month of collecting these algae. The GLs and fucoxanthin were isolated from extracts of using column chromatography with silica gel and their ROS-inhibitory activity was investigated too. The fatty acids (FAs) composition of lipids was determined by GC and GC/MS. It has been found that MGDG and DGDG stronger than SGDG inhibited the ROS and the degree of their activity depended on the species of algae, the month of collection, the amount of PUFA, the ratio of n − 3 and n − 6 PUFA in GLs. Fucoxanthin has shown a high degree of suppression of ROS. This preliminary study has shown the prospect of a deeper study of the suppression of ROS with the help of lipids from algae the Sea of Japan.

Unraveling the Lipidome and Antioxidant Activity of Native Bifurcaria bifurcata and Invasive Sargassum muticum Seaweeds: A Lipid Perspective on How Systemic Intrusion May Present an Opportunity

“…Interestingly, from a structural point of view, a distinct lipid profile between both seaweeds highlighted by lipid species rich in PUFA and also the higher PL and GL content obtained in S. muticum extract (PL and GL content, 0.25 ± 0.03 g/100 g DW and 1.31 ± 0.04 g/100 g DW, respectively), may justify the greatest antioxidant performance in this seaweed. The degree of inhibition of ROS was found to be correlated to the amounts of PUFA, the n −3 and n −6 PUFA ratios in the extracts, and with the GL classes MGDG and DGDG, showing greater antioxidant activities than SGDG [ 60 ]. Generally, PUFA and n -3 FA have been suggested to hold increased antioxidant potential with regard to other FA [ 61 ], and lipid extracts from S. muticum are significantly more abundant in these than those of B. bifurcata , what might concur to the increased antioxidant activity in extracts from the invasive species.…”