Application of Goldmag immune probe in timely detection of syphilis based on GIS platform

Abstract

The purpose of this study was to apply goldmag immunoprobes into establishment of nanoparticles-based colorimetric assay as well as construction of immunochromatography quantitative and qualitative system by exploring point-of-care testing of syphilis with goldmag particles carrier-based immunoprobe and analysis of spatial data of Geographic Information System (GIS) platform. Goat anti-rabbit immunoglobulin G (IgG) was coupled on the surface of modified nanoparticles, taking N-(3-dimethylaminopropyl)-N'-ethyl-carbodiimide as the connector. Then the nanoparticles were used for colorimetric detection of goat-anti-rabbit IgG in liquid phase system. Based on the analysis of spatial data in GIS platform, we found the probe constructed based on MUA-Fe304/Au nanoparticles responded more sensitive to detection objects compared with the probe designed based on PAA-FeO/Au nanoparticles, and its reaction rate constant was two times that of PAA-FeO/Au nanoparticles based goldmag immunoprobe. Goldmag particles not only can be coupled with biomolecules such as antibody/antigen and glycoprotein but also possess superparamagnetism.

Recent Advances in Nanosystems and Strategies for Vaginal Delivery of Antimicrobials

“…Recently, a novel quantum dot-based point of care test for syphilis has been developed [56]. Moreover, a Goldmag immune probe, as part of a NP-based colorimetric assay for the detection of syphilis, has also been proposed [57]. In addition, tests have demonstrated that polyelectrolyte-coated gold magnetic NPs are sensitive and selective when used in a syphilis immunoassay, suggesting a possible use in point of care diagnostics for syphilis screening [58].…”

Nanomedicine applications in women's health: state of the art

“…105 The potential application of Goldmag immune probes, as part of a NP-based colorimetric assay for the detection of syphilis, has also been indicated. 106 In addition, polyelectrolyte-coated gold magnetic NPs have been proven to be sensitive and selective when used in a syphilis immunoassay, suggesting a possible use in point of care diagnostics for syphilis screening. 107 Consequently, it is already evident that nanomedicine may benefit the health of women by minimizing the side effects of contraceptives.…”