Application of High-speed Countercurrent Chromatography for the Purification of High-purity Illudin S from Omphalotus japonicus

Abstract

Illudin S from mushroom, such as Omphalotus japonicus and illudens, is a natural sesquiterpene analog with strong antitumor and antiviral activities. These illudins compounds are highly effective against various drug-resistant cancers that show extreme cytotoxicity an in vitro assay. However, it is difficult to obtain a sufficient amount of highly pure illudin S from a natural product by simple, efficient and low-cost purification techniques. Here, we offer to apply the high-speed countercurrent chromatography for the preparative purification of illudin S from mushroom extract. For a two-solvent system, the optimal condition of hexane/ethyl acetate/methanol/water (1/5/1/5, v/v/v/v) was optimized to obtain pure illudin S from a crude extract. This purified component was evaluated by liquid chromatography (high-purity >99%) and tandem mass spectrometry. The yield amounts of illudin S (1.3 mg/about 10 g Omphalotus japonicus) at one running are determined by liquid chromatographic calibration. It is concluded that by requiring a natural material and costeffectiveness, our method represents a significant improvement over complicated techniques for the purification of illudin S from natural materials.

Simultaneous Determination of Multi-Class Mushroom Toxins in Mushroom and Biological Liquid Samples Using LC-MS/MS

“…The values for ibotenic acid and muscimol in serum and urine are not available, and the other 10 toxins showed MLOQs of 0.15~2.0 µg/L. These results were in line with or even lower than the majority of previous studies on mushroom toxins [19,[32][33][34]. A preliminary summary of the literature on the detection of mushroom toxins by LC-MS/MS is given in Table S6 in Supplementary Materials.…”

Partition Efficiency of Eccentric Coil for Countercurrent Chromatographic Separation of Proteins Using Small-scale Cross-axis Coil Planet Centrifuge with Circular and Elliptic Cylindrical Columns

“…The partition efficiency in each separation were evaluated by the peak resolution (Rs) computed from the chromatogram according to the conventional formula and the stationary phase retention directly measured after each separation. 3…”

“…1 The elimination of the solid support matrix provides the advantage especially for the separation and purification of natural products 2 over common column chromatography. 3 In order to achieve higher partition efficiency including the peak resolution and the stationary phase retention, many hydrostatic and hydrodynamic types of CCC instruments have been developed by a number of researchers. 4 In the hydrodynamic CCC machines, high-speed countercurrent chromatography (HSCCC) mainly including the type-J coil planet centrifuge (CPC) and the cross-axis CPC has been most effective for the separation of various chemical compounds as the best models.…”

Isolation of Momilactones A and B from Rice Husk Using High-Speed

“…Although traditional column chromatography has been employed for momilactone isolation, it is time-consuming and labor-intensive. Our previous experiences with HSCCC in isolating compounds such as avermectin from bacterial culture, illudin S from Omphalotus japonicus, and sesamin/sesamolin from sesame seed oil demonstrate its potential for efficient and sustainable isolation [14][15][16]. In this study, we aim to isolate MA and MB from discarded rice husk using HSCCC.…”