Application of hydrophilic–lipophilic balance (HLB) number to optimize a compatible non-ionic surfactant for dried aerial conidia of Beauveria bassiana

Jin, Xixuan; Streett, Douglas A.; Dunlap, Christopher A.; Lyn, Margaret E.

doi:10.1016/j.biocontrol.2008.03.008

Cited by 60 publications

(31 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2A). Thus, a quantitative estimate of the relative difference in (29) and Silwet (17). NA indicates surfactants for which an HLB value has not been conclusively determined.…”

Section: Methodsmentioning

confidence: 99%

Novel High-Throughput Detection Method To Assess Bacterial Surfactant Production

Burch

Shimada

Browne

et al. 2010

Appl Environ Microbiol

106

View full text Add to dashboard Cite

A novel biosurfactant detection assay was developed for the observation of surfactants on agar plates. By using an airbrush to apply a fine mist of oil droplets, surfactants can be observed instantaneously as halos around biosurfactant-producing colonies. This atomized oil assay can detect a wide range of different synthetic and bacterially produced surfactants. This method could detect much lower concentrations of many surfactants than a commonly used water drop collapse method. It is semiquantitative and therefore has broad applicability for uses such as high-throughput mutagenesis screens of biosurfactant-producing bacterial strains. The atomized oil assay was used to screen for mutants of the plant pathogen Pseudomonas syringae pv. syringae B728a that were altered in the production of biosurfactants. Transposon mutants displaying significantly altered surfactant halos were identified and further analyzed. All mutants identified displayed altered swarming motility, as would be expected of surfactant mutants. Additionally, measurements of the transcription of the syringafactin biosynthetic cluster in the mutants, the principal biosurfactant known to be produced by B728a, revealed novel regulators of this pathway.

show abstract

“…2A). Thus, a quantitative estimate of the relative difference in (29) and Silwet (17). NA indicates surfactants for which an HLB value has not been conclusively determined.…”

Section: Methodsmentioning

confidence: 99%

Novel High-Throughput Detection Method To Assess Bacterial Surfactant Production

Burch

Shimada

Browne

et al. 2010

Appl Environ Microbiol

106

View full text Add to dashboard Cite

show abstract

“…Biocompatibility is typically tested explicitly with potential surfactants, but there are general guidelines that can narrow one's search. Aerial conidia are generally much more tolerant of surfactants than blastospores, submerged conidia, or hyphal formulations and many successful examples of their use are available in the literature (Daoust et al 1983;Alves et al 2002;Akbar et al 2005;Faria and Wraight 2007;Jin et al 2008). Blastospores, submerged conidia, and hyphae lack the hydrophobic properties of aerial conidia, which allows surfactants to interact directly with the outer membrane of the cell.…”

Section: Formulations Adjuvants Adherence and Interactionsmentioning

confidence: 98%

Ecological considerations in producing and formulating fungal entomopathogens for use in insect biocontrol

2009

Self Cite

View full text Add to dashboard Cite

Insect pests persist in a wide-variety of agricultural, arboreal and urban environments. Effective control with fungal entomopathogens using inundation biocontrol requires an understanding of the ecology of the target insect, fungal pathogen, and the insect-pathogen interaction. Historically, the development of production and formulation processes for biocontrol fungi has primarily focused on reducing costs by maximizing the yield of infective propagules, increasing storage stability, and improving product form for ease of application. These goals are critical for commercialization but are often in conflict with environmental and ecological considerations. Critical parameters for selecting a fungal pathogen for use in inundation biocontrol include the cost-effective production of a stable, infective propagule that is suited for use in the environment where the insect must be controlled. Production processes can be manipulated nutritionally and environmentally to produce efficacious propagules or to direct fungal differentiation to propagule forms that may be better suited for use in specific environments. Formulation development must also consider ecological and environmental factors to maximize biocontrol efficacy. A basic understanding of the surface chemistries of the fungal propagule and insect, the interactions between a fungal propagule and the insect cuticle that lead to infection, and the impact of the environment on this interaction can aid in the development of effective formulations.

show abstract

“…Selanjutnya larva dapat berkembang secara normal di dalam umbi sehingga jumlah larva yang ditemukan pada perlakuan tersebut relatif lebih banyak. Oleh karena itu, untuk meningkatkan efikasi pengendalian menggunakan jamur B. bassiana diperlukan penambahan bahan perekat (Jin et al, 2008;Mishra et al, 2013;Mwanburi et al, 2015) atau dapat dikombinasikan dengan formulasi minyak (Ummidi & Vadlamani 2014) atau ekstrak minyak nabati (Rogerio et al, 2005;Silva et al, 2006;Mohan et al, 2007).…”

Section: A B Cunclassified

Perbandingan Metode Aplikasi Jamur Entomopatogen Beauveria Bassiana Untuk Pengendalian Cylas Formicarius (Coleoptera: Curculionidae)

Prayogo¹

2017

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

View full text Add to dashboard Cite

Comparing application methods of entomopathogenic fungus Beauveria bassiana to control Cylas formicarius (Coleoptera: Curculionidae). Cylas formicarius is a major pest of sweet potatoes. The tuber damage caused by C. formicarius reaches 100%. The research objective was to compare application methods of entomopathogenic fungi Beauveria bassiana to control C. formicarius. The research was conducted at the entomology screen house of Indonesian Legume and Tuber Crops Research Institute (ILETRI) at Malang, East Java, from January to September 2013. The treatment consisted of 12 application methods, each was repeated three times. The results showed that application of B. bassiana into the planting pit, soaking the sweet potato cuttings into the conidial suspension of B. bassiana for 30 minutes before planting, followed by spray application with two week interval (2 to 12 weeks after planting, WAP) or P4 was effective in suppressing C. formicarius population and tuber damage. Tuber weight obtained from P4 treatment was 1.4 kg plant -1 , while from the chemical insecticide was only 1.1 kg plant -1 . Tuber damage from P4 treatment was lower ( 5%) than that from chemical insecticide treatment (42%). Therefore, application of B. bassiana conidial suspension in the planting pit, continued by soaking the sweet potato cuttinginto the B. bassiana conidial suspension for 30 minutes before planting, and followed by spray applications of B. bassiana conidial suspension at two-week intervals ( 2-12 WAP) or P4 method can be recommended to control C. formicarius.Key words: adult, egg, larvae, tuber damage, tuber weight ABSTRAKPerbandingan metode aplikasi jamur entomopatogen Beauveria bassiana untuk pengendalian Cylas formicarius (Coleoptera:Ccurculionidae). Cylas formicarius merupakan hama utama ubi jalar. Kerusakan yang disebabkan C. formicarius mencapai 100%. Penelitian ini bertujuan untuk membandingkan metode aplikasi jamur entomopatogen, Beauveria bassiana dalam mengendalikan C. formicarius. Penelitian dilakukan di rumah kasa entomologi, Balai Penelitian Tanaman Aneka Kacang dan Umbi (BALITKABI), Malang pada bulan Januari sampai dengan September 2013. Perlakuan terdiri atas 12 metode aplikasi, masing-masing perlakuan diulang tiga kali. Hasil penelitian menunjukkan bahwa aplikasi konidia B. bassiana ke dalam lubang tanam, perendaman stek ke dalam suspensi konidia jamur B. bassiana selama 30 menit sebelum tanam, dilanjutkan aplikasi semprot dengan interval dua minggu mulai umur 2 sampai dengan 12 minggu setelah tanam (MST) (P4) efektif menekan populasi C. formicarius dan kerusakan umbi. Berat umbi perlakuan P4 mencapai 1,4 kg tanaman -1 , sedangkan perlakuan aplikasi insektisida kimia hanya 1,1 kg tanaman -1 . Kerusakan umbi perlakuan P4 hanya 5%, sedangkan kerusakan umbi pada aplikasi insektisida kimia mencapai 42%. Efikasi perlakuan P4 dapat dilihat dari populasi telur, larva maupun imago pada waktu panen lebih sedikit dibandingkan dengan perlakuan lainnya. Oleh karena itu, aplikasi suspensi konidia B. bassiana pada luba...

show abstract

Application of hydrophilic–lipophilic balance (HLB) number to optimize a compatible non-ionic surfactant for dried aerial conidia of Beauveria bassiana

Cited by 60 publications

References 35 publications

Novel High-Throughput Detection Method To Assess Bacterial Surfactant Production

Novel High-Throughput Detection Method To Assess Bacterial Surfactant Production

Ecological considerations in producing and formulating fungal entomopathogens for use in insect biocontrol

Perbandingan Metode Aplikasi Jamur Entomopatogen Beauveria Bassiana Untuk Pengendalian Cylas Formicarius (Coleoptera: Curculionidae)

Contact Info

Product

Resources

About