Application ofTrichoderma reeseiCellulase and Xylanase Promoters through Homologous Recombination for Enhanced Production of Extracellular β-Glucosidase I

Microbial β-Glucosidases: Screening, Characterization, Cloning and Applications

“…Similarly, T. reesei produces cellulase component (endoglucanase and exoglucanase) in high amount with excellent properties such as thermostability and catalytic efficiency but it lacks sufficient amount of β-glucosidase. Researchers therefore have heterologously expressed exogenous β-glucosidase genes in T. reesei under strong promoter in order to enhance its β-glucosidase activity so that cellulose hydrolysis rate was improved considerably [112,187,190,191,192]. Majority of recombinant fungal β-glucosidase belong to glycoside hydrolase family 3.…”

Isolation, Screening and Characterization of Cellulase Producing Bacterial Isolates from Municipal Solid Wastes and Rice Straw Wastes

“…showed maximum cellulase production at 35°C (Figure 3). However, temperature optima for cellulase production may vary from bacterial species to species [30,31] The cellulase production by bacterial isolates is controlled by the extracellular pH as the pH of the culture media strongly influences many enzymatic processes. We investigated the effects of initial pH of the media on the production of cellulase by the bacterial isolates.…”

Bioresources for Third-Generation Biofuels

“…This additional step reduces the efficiency of the process. Some improvements have been achieved by the engineering of cellulases from the Trichoderma genus fungi (Fukuda et al, 2006) and the utilization of microorganisms able to simultaneously express the cellulase and enzymes needed for the ethanol fermentation pathway, such as piruvate descarboxilases and alcohol dehydrogenases (Lu et al, 2006;van Zyl et al, 2007;Jegannathan et al, 2009;Rahman et al, 2009;van Dam et al, 2009). However, these improvements have still not generated an efficient and economically affordable process.…”