Application of IgY to sandwich enzyme-linked immunosorbent assays, lateral flow devices, and immunopillar chips for detecting staphylococcal enterotoxins in milk and dairy products

Jin, Wei; Yamada, Keiko; Ikami, Mai; Kaji, Noritada; Tokeshi, Manabu; Atsumi, Yusuke; Mizutani, Makoto; Murai, Atsushi; Okamoto, Akira; Namikawa, Takao; Baba, Yoshinobu; Ohta, Michio

doi:10.1016/j.mimet.2013.01.001

Cited by 44 publications

(25 citation statements)

References 38 publications

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“…Various immunosensing setups operating with optical [5][6][7][8][9][10][11][12][13][14][15][16], acoustic [17][18][19][20][21] or electrical [22,23] transduction modes have been designed to detect SEs and showed variable working range and sensitivity. The pg/mL detection level was recently reached using a microchannel device made of microscale pillars and fluorescence detection [24,25]. In this context, piezoelectric biosensors using quartz chips are particularly attractive since they provide quantitative information on biomolecular interactions at the solid -liquid interface in real time and label-free fashion and allow the detection of high molecular weight targets at the ng level [26].…”

Section: Introductionmentioning

confidence: 99%

Gold colloid-nanostructured surfaces for enhanced piezoelectric immunosensing of staphylococcal enterotoxin A

Haddada

Salmain

Boujday

2018

Sensors and Actuators B: Chemical

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Highlights Gold-and silicon-coated quartz sensors were nanostructured with gold nanoparticles  A capture layer comprising an anti-SEA antibody was built up on the sensor surface  Nanostructuration improved sensor response owing to better accessibility of antibodies  With a sandwich-type assay, the limit of detection was 1 ng SEA/mL in 25 min AbstractWe describe the use of gold nanoparticles (AuNP) as a nanostructuring agent on quartz crystal sensor chips to engineer staphylococcal enterotoxin A (SEA) piezoelectric biosensors with amplified response. AuNPs were assembled on gold-or silicon-coated quartz crystal sensor chips by a wet chemistry process involving their chemisorption to preformed thiol and amine terminated Self-Assembled Monolayers (SAMs). The purpose of this nanostructuration was to modify the topography of the surface and improve the accessibility of the binding sites on the surface of the sensor chips. Biointerfaces, comprising a polyclonal antibody against staphylococcal enterotoxin A (SEA), were further built up on these gold nanoparticle-coated sensors and their ability to capture SEA was monitored in real time with a quartz crystal microbalance with dissipation monitoring. It was found out that, although the surface density in capture antibody was similar on both nanostructured and planar sensors, the sensor response, expressed as frequency shift recorded during the binding of SEA to the antibody, was significantly higher for the nanostructured sensors as compared to the planar ones. All the same, the limit of detection was lower for the nanostructured sensors: 8 ng/mL vs 20 ng/mL for the planar sensors. This was rationalized by a possibly better accessibility of the antigen binding sites rather than a consequence of specific surface increase. Using a sandwich type assay, gold nanoparticles coated silicon quartz sensor chips provided the lowest limit of detection of ca. 1 ng/mL in a total assay time of 25 min. 2

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Section: Introductionmentioning

confidence: 99%

Gold colloid-nanostructured surfaces for enhanced piezoelectric immunosensing of staphylococcal enterotoxin A

Haddada

Salmain

Boujday

2018

Sensors and Actuators B: Chemical

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“…Camelid antibodies are considered a promising starting material for diagnostic and therapeutic antibodies. There are also efforts to use antibodies from chickens [23,24] or to raise antibodies using plant viruses [25].…”

Section: Techniques To Produce Diagnostic Antibodiesmentioning

confidence: 99%

Immunodiagnostics and Immunosensor Design

Gubala¹,

Klein²,

Templeton³

et al. 2016

IUPAC Standards Online

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This work compiles information on the principles of diagnostic immunochemical methods and the recent advances in this field. It presents an overview of modern techniques for the production of diagnostic antibodies, their modification with the aim of improving their diagnostic potency, the different types of immunochemical detection systems, and the increasing diagnostic applications for human health that include specific disease markers, individualized diagnosis of cancer subtypes, therapeutic and addictive drugs, food residues, and environmental contaminants. A special focus lies in novel developments of immunosensor techniques, promising approaches to miniaturized detection units and the associated microfluidic systems. The trends towards high-throughput systems, multiplexed analysis, and miniaturization of the diagnostic tools are discussed. It is also made evident that progress in the last few years has largely relied on novel chemical approaches.

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“…Conversely, in the absence (or lower than the cutoff value) of target analyte, numerous colored reagent-labeled analyte would bind on test zone, and two characteristic red lines can be observed from the test zone and control zone respectively. On the other hand, when the target analyte owns more than one specific antibodies or DNA aptamers, sandwich format could be employed to test the target analyte [64][65][66][67][68][69][70]33,71]. Figure 2 presents the typical DNA aptamers-based sandwich format.…”

Section: Lfb Assay Formats and Principlementioning

confidence: 99%

Recent Advances in Nanoparticles-based Lateral Flow Biosensors

Gao¹,

Xu²,

Zhou³

et al. 2014

Am. J. Biomed. Sci.

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Lateral flow biosensors (LFBs) provide advantages in low cost, simplicity, rapidity, stability and portability, thus making LFBs popular in biomedical, agriculture, food and environmental sciences. This article reviews recent advances of LFBs for bioassays, including the investigation of the improvements achieved by signal-amplification strategies, the application of new nanoparticle labels, novel quantitative system and simultaneous detection. We summarized the outstanding performances of LFBs such as high detection sensitivity, specificity, reproducibility and reliability.

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Application of IgY to sandwich enzyme-linked immunosorbent assays, lateral flow devices, and immunopillar chips for detecting staphylococcal enterotoxins in milk and dairy products

Cited by 44 publications

References 38 publications

Gold colloid-nanostructured surfaces for enhanced piezoelectric immunosensing of staphylococcal enterotoxin A

Gold colloid-nanostructured surfaces for enhanced piezoelectric immunosensing of staphylococcal enterotoxin A

Immunodiagnostics and Immunosensor Design

Recent Advances in Nanoparticles-based Lateral Flow Biosensors

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