Application of immunoassay in the food industry

Abstract

Immunoassay techniques using the highly specific and sensitive nature of immunological reactions have been developed and applied in the food industry for detecting the naturally occurring constituents, antibiotics, pesticide residues, microorganisms, and fragments of microbial constituents related to food analysis, food production, food processing, and food safety. Both polyclonal and monoclonal antibodies are employed for the development of the various immunoassay systems, including enzyme-linked immunoassay (ELISA) and radioimmunoassay (RIA). Immunoassay techniques provide complementary and/or alternate approaches in reducing the use of costly, sophisticated equipment and analysis time, but still maintaining reliability and improved sensitivity. Immunoassay techniques in their most simple forms provide excellent screening tools to detect adulteration and contaminations qualitatively. The application of immunoassay techniques contributes tremendously to the quality control and safety of our food supply.

Enzyme-linked Immunosorbent Assay for the Detection of Hen's Egg Proteins in Processed Foods

“…This change can affect antibody recognition toward antigen proteins (Breton et al, 1988;Samarajeewa et al, 1991). Therefore, we attempted to develop a reliable ELISA system for the quantification of egg proteins, regardless of heat treatment.…”

Immunostick Colorimetric ELISA Assay for the Identification of Smoked Salmon, Trout and Bream

“…Immunoassay techniques provide complementary and/or alternative approaches in reducing the use of costly, sophisticated equipment and analysis time, but still maintaining reliability and sensitivity (Smith D M 1995). Immunoassay techniques in their most simple forms provide excellent screening tools for detecting food adulteration (Samarajeewa et al 1991).…”

Et ve Et Ürünlerinde At ve Domuz Eti Varlığının Uhlenhuth Presipitasyon Halka, Agar Gel Immuno Diffuzyon ve EnzymeLinked Immuno Sorbent Assay Metotları ile Araştırılması

“…Antijen ve buna homolog presipitin agar jelinde birbirlerine doğru yayıldıkları zaman optimal yayılma bölgesinde bir presipitasyon çizgisi oluş-tururlar 1 . ELISA gibi immunoenzimatik yöntemler hassas, spesifik, kolay uygulanabilir ve hızlı olmaları ile tür tespitinde tercih edilmektedir 19 . ELISA, hileli hazırlanmış taze et karışımları ile ısıl işlem görmüş et ürünlerinde hayvan türü-nün tespiti için etkili bir metot olarak bildirilmektedir 3,20,21 .…”