Application of immunodiagnostic assays: detection of antibodies and circulating antigens in human schistosomiasis and correlation with clinical findings.

Al-Sherbiny, Maged; Osman, Ahmed; Hancock, Kathy; Deelder, André M.; Tsang, Victor C. W.

doi:10.4269/ajtmh.1999.60.960

Cited by 84 publications

(29 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In comparison, Tsang et al (15) used homologous adult microsomal antigens, which allowed the detection of the 30-kDa band highly specific for infection with S. mansoni (1) and the detection of the 23-kDa band specific for infection with S. haematobium (15). The WB technique with crude antigen from adult (11,16) and WB analysis as screening and confirmatory assays, respectively, for the detection of the species causing the infection (1). The WB technique with HAMA resulted in a sensitivity of 94% for S. haematobium egg-positive patients; the sensitivity increased to 100% when the WB technique was combined with FAST-ELISA (1).…”

Section: Discussionmentioning

confidence: 99%

“…The WB technique with HAMA resulted in a sensitivity of 94% for S. haematobium egg-positive patients; the sensitivity increased to 100% when the WB technique was combined with FAST-ELISA (1). The ELISA with MAMA was positive with 53 to 83% of sera from patients infected with S. haematobium (1,11). Detection of specific antibodies to MAMA and HAMA was found to be 100% specific for the detection of S. mansoni and S. haematobium, respectively, when they were used in the FAST-ELISA and immunoblot assays (1).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Development and Evaluation of a Western Blot Kit for Diagnosis of Schistosomiasis

Sulahian

Garin

Izri

et al. 2005

Clin Vaccine Immunol

View full text Add to dashboard Cite

We evaluated the performance of Western blot (WB) analysis using commercially available antigen strips and compared the results with those of indirect hemagglutination (IHA) and indirect immunofluorescence (IFAT) for the serodiagnosis of human schistosomiasis. The antigen preparation was a crude extract of Schistosoma mansoni. The WB profile characteristics of schistosomiasis were characterized by comparing the results for 58 serum samples from patients with parasitologically proven S. mansoni (n ‫؍‬ 12) and S. haematobium (n ‫؍‬ 46) infections and 37 individuals with probable cases of schistosomiasis but with only positive serology results. The specificity of WB analysis was assessed by testing 12 serum samples from healthy subjects, 67 serum samples from patients with other proven helminthic and protozoan infections, and 16 serum samples from patients with autoantibodies. Six immunodominant bands (65, 70, 80, 95, 110, and 120 kDa) were revealed with sera from patients with schistosomiasis. The presence of three or more bands in the range 65 to 120 kDa, with the exception of the 100-kDa band, was considered diagnostic for Schistosoma infection and had a specificity of 100% in our series. In patients with proven schistosomiasis, the sensitivity of WB analysis was 84.5%, whereas those of IFAT and IHA were 65.5 and 72.9%, respectively. For serologically proven cases, the sensitivity of WB analysis was 97.3%. The overall sensitivity and specificity for both groups of patients were 89.5 and 100%, respectively, with positive and negative predictive values of 100 and 91.3%, respectively. We conclude that WB analysis is a useful technique for the immunological diagnosis of schistosomiasis.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Development and Evaluation of a Western Blot Kit for Diagnosis of Schistosomiasis

Sulahian

Garin

Izri

et al. 2005

Clin Vaccine Immunol

View full text Add to dashboard Cite

show abstract

“…The purified antigens of adult worms have allowed the obtainment of microsomal antigens of S. mansoni (MAMA) and of S. haematobium with sensitivities of 96 and 98% for the diagnosis of schistosomiasis due to S. mansoni and S. haematobium, respectively (3,19). Nonetheless, the diagnostic sensitivity for cases produced by other species is lower.…”

Section: Figmentioning

confidence: 99%

“…Nonetheless, the diagnostic sensitivity for cases produced by other species is lower. The ELISA for MAMA permits the diagnosis of only 55 to 83% of the cases caused by S. haematobium (3,19). This fact limits the potential of using only one of these ELISAs for the diagnosis of imported schistosomiasis, especially among travelers and immigrants coming from Africa (9,26).…”

Section: Figmentioning

confidence: 99%

See 1 more Smart Citation

Utility ofSchistosoma bovisAdult Worm Antigens for Diagnosis of Human Schistosomiasis by Enzyme-Linked Immunosorbent Assay and Electroimmunotransfer Blot Techniques

Pardo

Carranza

Turrientes

et al. 2004

Clin Vaccine Immunol

View full text Add to dashboard Cite

Immunodiagnostic methods based on the detection of antibodies continue to be the most effective and practical methods for the diagnosis of imported schistosomiasis. Schistosoma bovis is a species whose final natural hosts are bovines, ovines, caprines, and small wild ruminants. Different studies have demonstrated the analogies existing between S. bovis and other Schistosoma species which affect humans. The objective of this work was to evaluate the utility of S. bovis adult worm antigens (AWA) for the diagnosis of imported human schistosomiasis by enzyme-linked immunosorbent assay (ELISA) and electroimmunotransfer blotting (EITB) techniques. By detecting eggs, the ELISA for S. bovis AWA was able to definitively detect imported cases with a sensitivity of 94%. The specificity of the ELISA for S. bovis AWA was 97%. There were no differences between the results of the S. bovis AWA ELISA for patients infected with Schistosoma mansoni and those infected with Schistosoma haematobium. The EITB technique showed bands of 85, 37, and 20 kDa, which are characteristic of infections with Schistosoma spp. Specific bands to indicate infection by different species of Schistosoma have not been detected. The combined use of the ELISA for S. bovis AWA and EITB increased the global sensitivity of the study to 97%. Our findings suggest that the ELISA for S. bovis AWA is a useful test for the immunodiagnosis of imported schistosomiasis and that EITB for detecting S. bovis AWA permits the confirmation of diagnosis when the ELISA for S. bovis AWA is positive.

show abstract

Circulating antigen tests and urine reagent strips for diagnosis of active schistosomiasis in endemic areas

Ochodo

Gopalakrishna

Spek

et al. 2015

Cochrane Database of Systematic Reviews

131

View full text Add to dashboard Cite

Background Point‐of‐care (POC) tests for diagnosing schistosomiasis include tests based on circulating antigen detection and urine reagent strip tests. If they had sufficient diagnostic accuracy they could replace conventional microscopy as they provide a quicker answer and are easier to use. Objectives To summarise the diagnostic accuracy of: a) urine reagent strip tests in detecting active Schistosoma haematobium infection, with microscopy as the reference standard; and b) circulating antigen tests for detecting active Schistosoma infection in geographical regions endemic for Schistosoma mansoni or S. haematobium or both , with microscopy as the reference standard. Search methods We searched the electronic databases MEDLINE, EMBASE, BIOSIS, MEDION, and Health Technology Assessment (HTA) without language restriction up to 30 June 2014. Selection criteria We included studies that used microscopy as the reference standard: for S. haematobium , microscopy of urine prepared by filtration, centrifugation, or sedimentation methods; and for S. mansoni , microscopy of stool by Kato‐Katz thick smear. We included studies on participants residing in endemic areas only. Data collection and analysis Two review authors independently extracted data, assessed quality of the data using QUADAS‐2, and performed meta‐analysis where appropriate. Using the variability of test thresholds, we used the hierarchical summary receiver operating characteristic (HSROC) model for all eligible tests (except the circulating cathodic antigen (CCA) POC for S. mansoni , where the bivariate random‐effects model was more appropriate). We investigated heterogeneity, and carried out indirect comparisons where data were sufficient. Results for sensitivity and specificity are presented as percentages with 95% confidence intervals (CI). Main results We included 90 studies; 88 from field settings in Africa. The median S. haematobium infection prevalence was 41% (range 1% to 89%) and 36% for S. mansoni (range 8% to 95%). Study design and conduct were poorly reported against current standards. Tests for S. haematobium Urine reagent test strips versus microscopy Compared to microscopy, the detection of microhaematuria on test strips had the highest sensitivity and specificity (sensitivity 75%, 95% CI 71% to 79%; specificity 87%, 95% CI 84% to 90%; 74 studies, 102,447 participants). For proteinuria, sensitivity was 61% and specificity was 82% (82,113 participants); and for leukocyturia, sensitivity was 58% and specificity 61% (1532 participants). Howeve...

show abstract

Application of immunodiagnostic assays: detection of antibodies and circulating antigens in human schistosomiasis and correlation with clinical findings.

Cited by 84 publications

References 22 publications

Development and Evaluation of a Western Blot Kit for Diagnosis of Schistosomiasis

Development and Evaluation of a Western Blot Kit for Diagnosis of Schistosomiasis

Utility ofSchistosoma bovisAdult Worm Antigens for Diagnosis of Human Schistosomiasis by Enzyme-Linked Immunosorbent Assay and Electroimmunotransfer Blot Techniques

Circulating antigen tests and urine reagent strips for diagnosis of active schistosomiasis in endemic areas

Contact Info

Product

Resources

About