Application of in vitro anthelmintic sensitivity assays to canine parasitology: Detecting resistance to pyrantel in Ancylostoma caninum

Kopp, Steven; Coleman, Glen; McCarthy, James S.; Kotze, Andrew C.

doi:10.1016/j.vetpar.2007.12.020

Cited by 60 publications

(84 citation statements)

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“…Two isolates of A. caninum were used for these experiments: an isolate with high-level resistance to pyrantel, designated isolate PR, and an isolate with low-level resistance to pyrantel, designated isolate NT. These isolates have previously been described in detail (11). Briefly, isolate PR was sourced from a pool of isolates recovered from dogs in metropolitan Brisbane, Australia, while isolate NT was sourced from a puppy in an aboriginal community in the Northern Territory, Australia.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, isolate PR was sourced from a pool of isolates recovered from dogs in metropolitan Brisbane, Australia, while isolate NT was sourced from a puppy in an aboriginal community in the Northern Territory, Australia. A direct in vivo comparison of these isolates by the use of an abbreviated critical trial (7) revealed that the clinical efficacy of pyrantel was 25% against isolate PR and 71% against isolate NT on the basis of the proportion of adult worms expelled during the trial (11).…”

Section: Methodsmentioning

confidence: 99%

“…Larvae and adult worms were recovered as described previously (11). Briefly, larvae at the L3 stage (L3 larvae) were cultured from feces and stored at room temperature in BU buffer (50 mM Na 2 HPO 4 , 22 mM KH 2 PO 4 , 70 mM NaCl, pH 6.8) containing 10 l/ml penicillin (5,000 U/ml)-streptomycin (5 mg/ml) and 100 l/ml amphotericin B (250 g/ml) for no more than 2 weeks before use in the assays.…”

Section: Methodsmentioning

confidence: 99%

“…A larval migration assay has been shown to discriminate between pyrantel-susceptible and -resistant isolates of the porcine nematode Oesophagostomum dentatum (19), and a larval development assay has been reported to be able to detect pyrantel resistance in some livestock species (15). In addition, a larval motility assay (LMA) has been observed to discriminate between isolates of the canine hookworm (Ancylostoma caninum) with different susceptibilities to pyrantel, but the biphasic nature of the dose-response curves obtained by this assay complicates mathematical interpretation (11). Recently, a novel bioassay, the larval arrested morphology assay (LAMA), has been developed for the detection of resistance to pyrantel in A. caninum (11).…”

mentioning

confidence: 99%

“…In addition, a larval motility assay (LMA) has been observed to discriminate between isolates of the canine hookworm (Ancylostoma caninum) with different susceptibilities to pyrantel, but the biphasic nature of the dose-response curves obtained by this assay complicates mathematical interpretation (11). Recently, a novel bioassay, the larval arrested morphology assay (LAMA), has been developed for the detection of resistance to pyrantel in A. caninum (11). While more work is required to properly assess the performance of this assay with a comprehensive range of isolates, it shows promise as a tool for the detection of resistance to pyrantel in hookworm species.…”

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confidence: 99%

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Phenotypic Characterization of Two Ancylostoma caninum Isolates with Different Susceptibilities to the Anthelmintic Pyrantel

Kopp

Coleman

McCarthy

et al. 2008

Antimicrob Agents Chemother

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The anthelmintic pyrantel plays an important role in the control of gastrointestinal helminths of humans and domestic animals. Despite the demonstration of pyrantel resistance in several helminth species over the last 20 years, the resistance mechanism remains unclear. It has been hypothesized that resistance may arise as a consequence of changes to the relative proportions of subpopulations of nicotinic acetylcholine receptors (nAchRs). To test this hypothesis, we examined the responses of two isolates of the canine hookworm Ancylostoma caninum with low-level resistance (isolate NT) and high-level resistance (isolate PR) to pyrantel to nicotinic agonist drugs reported to be selective for three nAchR subtypes. We used larval motility and conformation assays and force transduction experiments with adult worms. Pyrantel and levamisole were less potent against larvae of isolate PR than larvae of isolate NT (up to an 18-fold increase in the 50% inhibitory concentration); on the other hand, bephenium was more potent against larvae of isolate PR than larvae of isolate NT (twofold) and nicotine had the same potency against larvae of both isolates. In adults, pyrantel, levamisole, and nicotine were less potent against isolate PR than isolate NT (two-to threefold), but the potency of bephenium against the two isolates was equivalent. Our data indicate a complex pattern of nAchRs in this species and suggest that the two isolates differ in their relative sensitivities to agonists targeting different nAchRs.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Phenotypic Characterization of Two Ancylostoma caninum Isolates with Different Susceptibilities to the Anthelmintic Pyrantel

Kopp

Coleman

McCarthy

et al. 2008

Antimicrob Agents Chemother

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Ancylostoma caninum and Other Canine Hookworms

Hawdon

Wise

2020

Parasitology Research Monographs

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Characterization of a multidrug resistant Teladorsagia circumcincta isolate from Spain

et al. 2011

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The aim of this work was to know the anthelmintic resistance (AR) status of a Spanish sheep flock infected by gastrointestinal nematodes (GIN) and the possible cross resistance among anthelmintics of the macrocyclic lactones (ML) family. The Faecal Egg Count Reduction Test (FECRT) was carried out to check the efficacy of albendazole (Zodalben®), levamisole (LEV) (Endex®) and an oral formulation of ivermectin (IVM) (Oramec®), at the recommended dose rates. Then, the study was extended to check the cross resistance between drugs of the ML family: injectable IVM (Ivomec®), oral moxidectin (Cydectin®), injectable moxidectin (Biodectin®) and doramectin (Dectomax®), at the recommended dose rates. The GIN species were identified after faecal cultures in all groups. The FECRT showed the resistance of a Teladorsagia circumcincta isolate against LEV (39-58%), IVM (88-92%) and doramectin (85%). This study is the first report to confirm the side resistance between these MLs, which belong to the avermectin chemical group, in a Spanish sheep flock. The in vitro efficacy of LEV and IVM was measured by the Larval Feeding Inhibition Assay (LFIA) using the IC(50) measurement (concentration needed to inhibit the ingestion of 50% L1). The values of the multidrug resistant isolate were 0.25 μg/ml for LEV and 3 ng/ml for IVM. Both results were higher than the values obtained with a susceptible isolate, which could be indicative of AR. However, further research examining the response of a greater range of susceptible and resistant nematodes isolates should be carried out to establish a discrimination threshold.

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Application of in vitro anthelmintic sensitivity assays to canine parasitology: Detecting resistance to pyrantel in Ancylostoma caninum

Cited by 60 publications

References 32 publications

Phenotypic Characterization of Two Ancylostoma caninum Isolates with Different Susceptibilities to the Anthelmintic Pyrantel

Phenotypic Characterization of Two Ancylostoma caninum Isolates with Different Susceptibilities to the Anthelmintic Pyrantel

Ancylostoma caninum and Other Canine Hookworms

Characterization of a multidrug resistant Teladorsagia circumcincta isolate from Spain

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