Application of in vitro pollination, ovary culture, ovule culture and embryo rescue for overcoming incongruity barriers in interspecific Lilium crosses

Tuyl, J.M. van; Dien, M.P. van; Creij, M.G.M. van; Kleinwee, T.C.M. Van; Franken, John; Bino, R.J.

doi:10.1016/0168-9452(91)90262-7

Cited by 191 publications

(101 citation statements)

References 24 publications

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“…The successfully fertilized ovules were recognizable by their increased size. The embryosacs containing embryos or the embryo only were excised from the ovules and placed in enriched media according to Van Tuyl et al (1991).…”

Section: Methodsmentioning

confidence: 99%

Progenies of allotriploids of Oriental × Asiatic lilies (Lilium) examined by GISH analysis

Barba-González¹,

Silfhout

Visser

et al. 2006

Euphytica

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With the aim of utilizing allotriploid (2n = 3x = 36) lily hybrids (Lilium) in introgression breeding, different types of crosses were made. First, using diploid Asiatic lilies (2n = 2x = 24), reciprocal crosses (3x − 2x and 2x − 3x) were made with allotriploid hybrids (AOA) obtained by backcrosses of F 1 Oriental × Asiatic hybrids (OA) to Asiatic cultivars (A). Secondly, the AOA allotriploids were crossed with allotetraploid (OAOA, 2n = 4x = 48), in 3x − 4x combination. Finally, the AOA allotriploids where crossed to 2n gamete producer F 1 OA hybrids (3x − 2x(2n)). Two types of triploids were used as parents in the different types of crosses, derived from: (a) mitotic polyploidization and (b) sexual polyploidization. Ploidy level of the progeny was determined by R. Barba-Gonzalez Centro de Investigación y Asistencia en Tecnología y, Diseño del Estado de Jalisco, A.C. Biotecnología Vegetal, Av. Normalistas #800, Colinas de la Normal, Guadalajara, Jalisco, C.P. estimating the DNA values through flowcytometry as well as chromosome counting. The aneuploid progeny plants from 3x − 2x and reciprocal crosses had approximate diploid levels and in 3x − 4x crosses and 3x − 2x (2n) the progeny had approximate tetraploid levels. Balanced euploid gametes (x, 2x and 3x) were formed in the AOA genotypes. Recombinant chromosomes were found in the progenies of all crosses, except in the case of 2x − 3x crosses through genomic in situ hybridization (GISH) analyses. Recombinant chromosomes occurred in the F 1 OA hybrid when the triploid AOA hybrid was derived through sexual polyploidization, but not through mitotic polyploidization with two exceptions. Those recombinant chromosomes were transmitted to the progenies in variable frequencies.

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Section: Methodsmentioning

confidence: 99%

Progenies of allotriploids of Oriental × Asiatic lilies (Lilium) examined by GISH analysis

Barba-González¹,

Silfhout

Visser

et al. 2006

Euphytica

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“…Interspecific hybridization is often prevented by a cross-incompatibility barrier, but such barriers can partly be overcome by embryo rescue techniques such as embryo, ovule, and immature seed cultures. Interspecific and intergeneric hybrids have successfully been obtained by embryo rescue techniques in a wide range of ornamental plants, Lilium (Obata et al, 2000;Van Tuyl et al, 1991), Zantedeschia (Yao et al, 1994), Primula (Amano et al, 2006;Kato et al, 2008), Colchicaceae plant (Amano et al, , 2009, and Cyclamen (Ishizaka, 2008). In Lychnis, only one viable hybrid plantlet has been obtained successfully between…”

Section: Introductionmentioning

confidence: 99%

Cross-compatibility in Interspecific Hybridization of Lychnis (Caryophyllaceae) and Characterization of Interspecific Hybrids between L. fulgens and L. sieboldii

Nakano

Kuwayama

Oka

et al. 2013

J. Japan. Soc. Hort. Sci.

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Lychnis belongs to Caryophyllaceae and contains a number of horticulturally attractive species. In order to widen their variations in horticultural traits, interspecific cross-pollination and subsequent immature seed culture were carried out using 8 Lychnis species, L. chalcedonica, L. coronata, L. fulgens, L. gracillima, L. kiusiana, L. miqueliana, L. sieboldii, and L. wilfordii. Enlarged fruits containing immature seeds were obtained in the 26 cross-combinations 4 weeks after pollination. Immature seeds were isolated from the fruits and cultured on halfstrength Murashige and Skoog medium without plant growth regulators, on which germination occurred in 11 cross-combinations and seedlings were produced in 8 cross-combinations. However, green seedlings were obtained only in L. fulgens × L. sieboldii, and seedlings obtained from the other 7 cross-combinations,kiusiana, and L. wilfordii × L. sieboldii, were albino and died during acclimatization. The hybridity of all 55 green seedlings obtained from L. fulgens × L. sieboldii was confirmed by random amplified polymorphic DNA analysis. Although some morphological variations were observed among hybrids, hybrid plants generally showed intermediate morphologies between the parents. Hybrids showed high pollen fertility and their selfpollination yielded viable seeds. The present study shows the possibility of interspecific cross-breeding in Lychnis. Further studies are necessary to improve the culture conditions of immature seeds and to examine interspecific cross-compatibility using a wide range of Lychnis species.

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“…There are only a few published papers about in vitro interspecific pollination (Van Tuyl et al, 1991;Janson, 1993;Chi, 2000). This work is the first successful attempt to produce Centaurium interspecies hybrid plants via sexual reproduction, and it was done under in vitro conditions.…”

Section: In Vitro Flowering and Visual Verification Of Interspecies Hmentioning

confidence: 99%

Interspecific in vitro hybridization in genus Centaurium and identification of hybrids via flow cytometry, RAPD, and secondary metabolite profiles

Banjanac¹,

Šiler²,

Škorić³

et al. 2014

Turk J Bot

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Most of the species from the genus Centaurium Hill readily hybridize between themselves in natural habitats. Artificial interspecific hybrid plants were obtained under in vitro conditions using reciprocal crosses between 4 species: Centaurium erythraea, C. littorale, C. maritimum, and C. pulchellum. Among 76 crossing experiments performed, 3 types of crosses produced seeds and gave 20 interspecific hybrid plants. Nuclear DNA quantification, fingerprinting of molecular markers, and determination of variation in main secondary metabolite content were employed to confirm their hybrid nature. All 3 approaches were capable of detecting hybrid plants. RAPD molecular markers proved to be the most reliable, always placing the hybrids between their parents in dendrograms, while secoiridoid glycoside content also showed intermediate profiles in the hybrid plants in relation to the parents. Hence, each of the techniques employed can be recommended for elucidating the mechanisms of interspecific hybridization in natural populations of Centaurium species in further studies. This could help to comprehend their breeding system and recognize interspecific hybridization as one of the crucial steps in polyploid formation and speciation in this genus.

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Application of in vitro pollination, ovary culture, ovule culture and embryo rescue for overcoming incongruity barriers in interspecific Lilium crosses

Cited by 191 publications

References 24 publications

Progenies of allotriploids of Oriental × Asiatic lilies (Lilium) examined by GISH analysis

Progenies of allotriploids of Oriental × Asiatic lilies (Lilium) examined by GISH analysis

Cross-compatibility in Interspecific Hybridization of Lychnis (Caryophyllaceae) and Characterization of Interspecific Hybrids between L. fulgens and L. sieboldii

Interspecific in vitro hybridization in genus Centaurium and identification of hybrids via flow cytometry, RAPD, and secondary metabolite profiles

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