Application of isoelectric focusing for studies of major histocompatibility complex class I antigen expression on human carcinomas and sarcomas

Wang, P.; Vánky, Farkas; Klein, Eva

doi:10.1007/bf01742531

Cited by 6 publications

(2 citation statements)

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“…In two tumors two bands were selectively weaker; 5164 bands 3 and 4 ( Fig. 1) and tumor 824 bands 1 and 3 (Wang et al, 1991). Sixteen tumors expressed all identified antigens.…”

Section: Hla-class-i Antigen Expression In Tumors Compared With Bloodmentioning

confidence: 94%

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Expression of MHC-class-I antigens in human carcinomas and sarcomas analyzed by isoelectric focusing

Wang

Vánky

et al. 1991

Int. J. Cancer

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We analyzed the expression of class-I antigens in ex vivo human tumor cells by isoelectric focusing (IEF) the anti-class-I mAb W6/32 immunoprecipitates prepared from cell lysates. Out of 42 experiments, 27 were technically successful. The patient's blood lymphocytes were used as controls. In vitro exposure of the tumor cells to IFNy and TNFa elevated class-I antigen expression. In I I cases, defects in MHC-class-I-antigen expression were observed. In 2 cases the antigens were detected only in the cytokine-treated tumor samples, probably due to a defect in the association between b2m and class-I heavy chains. Selective changes in the expression of alleles were seen in 10 cases and might involve HLA A, 6 and C antigens. Alterations in class-I expression as compared with the lymphocytes were observed in 9 of I 3 cases in which the tumor cells were collected from metastases, and only in 2 of I 4 primary tumors.

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“…In two tumors two bands were selectively weaker; 5164 bands 3 and 4 ( Fig. 1) and tumor 824 bands 1 and 3 (Wang et al, 1991). Sixteen tumors expressed all identified antigens.…”

Section: Hla-class-i Antigen Expression In Tumors Compared With Bloodmentioning

confidence: 94%

“…A prerequisite for analysis of HLA expression by IEF applied to tumor cells is the availability of metabolically active cell suspensions with minimal contamination by non-malignant cells, particularly leucocytes (Wang et al, 1991). Technically satisfactory analysis was achieved in over half of the specimens we processed.…”

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confidence: 99%