Application of ISSRs for cultivar identification and assessment of genetic relationships in rose

Abstract

The inter-simple sequence repeat (ISSR) technique was evaluated for its applicability to cultivar identification and assessment of genetic relationships in rose. Nine ISSR primers that revealed informative patterns were selected to fingerprint (by Resophor agarose gel electrophoresis) 33 cultivars, including unrelated cultivars, sports and offspring obtained by sexual propagation from the same initial variety. A total of 159 fragments were generated using these nine primers, 149 of which (93.7%) were polymorphic. All 33 rose cultivars, except for the known colour sports, were differentiated. A Jaccard's dissimilarity coefficient matrix of the cultivars showed a low dissimilarity level between them (mean dissimilarity: 0.45). An unweighted pair group method with the arithmetic averages dendrogram and principal coordinates analysis separated them into four main groups according to their horticultural classification and pedigree. The ISSR technique is therefore a potentially useful tool for cultivar identification and assessing genetic relationships in rose because it is simple, fast, cost-effective, reliable and highly discriminating

Molecular and Phenotypic Characterization of Advanced Backcross Lines Derived from Interspecific Hybridization of Durum Wheat

“…The microsatellite markers Xgwm (69) and Xwmc (24, 67) were used for characterization of both parental and backcross lines, as well as the additional genotypes included in the interspesific crosses ( Table 1) For ISSR analysis three ISSR primers (GA) 9 c, (ct) 9 G and (AC) 8 G were used. ISSR amplification was conducted in a 20 μL volume containing 75 ng of genomic DNA, 1.25 U Taq DNA polymerase, 1.5 mmol/L MgCl 2 , 0.2 mmol/L dNTPs, and 0.1 μmol/L primer.…”

“…ISSRs (Inter Simple Sequence Repeats) have been also proved to be useful in cultivar identification, genetic diversity and alien gene introgression studies (9,46,62,98). ISSRs detect polymorphisms in inter-microsatellite loci, using a single primer composed of an SSR sequence anchored at the 3' or 5' end by 1-4 arbitrary nucleotides.…”

“…Three ISSR markers (GA) 9 c, (Ac) 8 G and (CT) 9 G were selected on the basis of their wide application in studies on cultivar discrimination in wheat and its close relatives (62,98).…”

ISSR-derived Molecular Markers for Korean Wheat Cultivar Identification

“…ISSR markers were also used for identification of abiotic stress like drought (Deshmukh et al 2012). In addition, SSR and ISSRs markers are useful for cultivar identification (Crespel et al 2009;Lu et al 2009), specifically ISSR markers have been used for wheat cultivar identification (Nagaoka and Ogihara 1997) including others such as radish (Liu et al 2008), lotus , and rose (Crespel et al 2009). …”

Assessment of genetic diversity of rose genotypes using ISSR markers

“…To fulfill the need for effective, accurate, and fast identification tools for rose varieties, application of several molecular marker systems has been performed. Different DNA fingerprinting techniques such as random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), simple sequence repeats (SSRs), and intersimple sequence repeats (ISSRs) have been used for effective characterization of rose varieties (Zhang et al, 2006;Crespel et al, 2009). In recent years, as a microsatellite-based technique, ISSRs are widely used PCR-based marker systems that provide valuable tools for genetic characterization of different organisms, especially in plants.…”