Land ll leachate have become a major public health concern because of their adverse effects on health.Due to its complex composition, the toxicological effects have yet to be evaluated. In this study, we use two model organisms: zebra sh and mung beans, to assess the toxic effects of land ll leachate. The results showed that low concentrations of waste leachate promoted the growth of mung beans, while high concentrations severely affected the growth and development of seedlings. Furthermore, land ll leachate caused a decrease in chlorophyll levels and malondialdehyde levels increased, signi cantly increased the rate of root tip micronuclei. In addition, zebra sh embryos exposed with 0.5%, 1%, 1.2%, 1.5% (v/v) land ll leachate, which was shown signi cantly reduced levels of embryonic incubation rate and heart rate, while the rates of mortality and malformation were increased. 1.0% of the land ll leachate in the experiment can result in a decrease in spontaneous movement frequency of embryos and the light stimulation reaction. The number of black and white area explore and mirror attacks were reduced. In general, these results help to understand the environmental toxicity of the land ll leachate, providing additional reference data for the risk assessment and management of land ll leachate.