Application of lipase immobilized on a hydrophobic support for the synthesis of aromatic esters

Abstract

The present study aimed at preparing three biocatalysts via physical adsorption of lipases from Candida rugosa (CRL), Mucor javanicus, and Candida sp. on a hydrophobic and mesoporous support (Diaion HP-20). These biocatalysts were later applied to the synthesis of aromatic esters of apple peel and citrus (hexyl butyrate), apple and rose (geranyl butyrate), and apricot and pineapple (propyl butyrate). Scanning electron microscopy and gel electrophoresis confirmed a selective adsorption of lipases on Diaion, thus endorsing simultaneous immobilization and purification. Gibbs free energy (G) evinced the spontaneity of the process (-17.9 kJ/mol ≤ G ≤ -5.1 kJ/mol). Maximum immobilized protein concentration of 30 mg/g support by CRL. This biocatalyst was the most active in olive oil hydrolysis (hydrolytic activity of 126.0 ± 2.0 U/g) and in the synthesis of aromatic esters. Maximum conversion yield of 89.1% was attained after 150 Min for the synthesis of hexyl butyrate, followed by the synthesis of geranyl butyrate (87.3% after 240 Min) and propyl butyrate (80.0% after 150 Min). CRL immobilized on Diaion retained around 93% of its original activity after six consecutive cycles of 150 Min for the synthesis of hexyl butyrate.

Production of a fermented solid containing lipases from Penicillium roqueforti ATCC 10110 and its direct employment in organic medium in ethyl oleate synthesis

“…Lipases are versatile enzymes, very important in the industry due to their use as a catalyst in the synthesis of several industrial processes, such as detergent supply, 1 depolymerization of polyesters and environmental remediation, 2–4 synthesis of aroma and biodiesel esters, 5,6 butters, and cosmetics production, 7 in addition to vitamin C synthesis 8 . This enzyme may be produced by microorganisms, animals, and plants 9–11 .…”

An overview on the conversion of glycerol to value‐added industrial products via chemical and biochemical routes

“…to catalyze various reactions, such as transesterification [22,26], hydrolysis, or synthesis of ester bonds [23,27], amination [24], alcoholysis, as well as the synthesis of acids and optically active lactones [25], among others [24,49,[55][56][57][58][59][60][61][62][63][64][65][66][67][68][69].…”

Candida Rugosa Lipase Immobilized on Hydrophobic Support Accurel MP 1000 in the Synthesis of Emollient Esters

“…The hydrolytic activity (HA) of crude free CRL and immobilized lipase (CRL-AMP) was determined on the hydrolysis of olive oil emulsion (Carvalho et al, 2017;Dos Santos et al, 2020). The emulsion was prepared by mixing 2.5 g of olive oil with 2.5 g of Arabic Gum solution at 7% m/v and 5 mL of 100 mmol L -1 buffer sodium phosphate pH 7.0.…”