Application of Lipopeptide Biosurfactant Isolated from a Halophile: Bacillus tequilensis CH for Inhibition of Biofilm

Abstract

Biosurfactants are amphiphilic molecules having hydrophobic and hydrophilic moieties produced by various microorganisms. These molecules trigger the reduction of surface tension or interfacial tension in liquids. A biosurfactant-producing halophile was isolated from Lake Chilika, a brackish water lake of Odisha, India (19°41'39″N, 85°18'24″E). The halophile was identified as Bacillus tequilensis CH by biochemical tests and 16S rRNA gene sequencing and assigned accession no. KC851857 by GenBank. The biosurfactant produced by B. tequilensis CH was partially characterized as a lipopeptide using thin-layer chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance techniques. The minimum effective concentration of a biosurfactant for inhibition of pathogenic biofilm (Escherichia coli and Streptococcus mutans) on hydrophilic and hydrophobic surfaces was found to be 50 μg ml(-1). This finding has potential for a variety of applications.

Biosurfactants: promising bioactive molecules for oral-related health applications

“…It is worth mentioning that nuclear magnetic resonance (NMR) spectroscopy should be used for full characterisation of macromolecules such as lipoprotein. Also techniques such as 1D H 1 -NMR or C 13 -NMR are frequently used to characterise low molecular weight molecules, such as lipopeptide acting against S. mutans biofilm, for example, as reported by Pradhan et al (2013). However, such techniques should be used alongside other techniques such as HPLC-MS and in all cases compounds with high purity are needed especially in the case In a relatively close area, Rodrigues et al investigated a biosurfactant type described as glycoprotein from the probiotic bacteria Lactococcuslactis53 and S. thermophiles A.…”

Lipopeptides: Biosynthesis and Applications

“…Pradhan et al [46] isolated a biosurfactant producing halophile from Lake Chilika, Odisha, India. The halophile was identified as Bacillus tequilensis CH.…”

Effects of <i>Bacillus cereus</i> F-6 on Promoting Vanilla (<i>Vanilla planifolia</i> Andrews.) Plant Growth and Controlling Stem and Root Rot Disease

“…The isolated strain (F-6) was inoculated into an optimized Landy medium [20] [21] and then shaken at a speed of 170 rpm at 28˚C for 24 h. Lipopeptides were extracted by following the method of Pradhan et al [22] with minor modifications. The pH of the supernatant was adjusted to 2 with concentrated HCl.…”