Application of liquid-based cytology cervical cytologic specimens to cell block for histologic observation

Liquid‐based cytology and cell block immunocytochemistry in veterinary medicine: comparison with standard cytology for the evaluation of canine lymphoid samples

“…23 With advances in LBC in human medicine, the method has been largely used, since it is a versatile option for the detection of antigens by immunocytochemistry. 14,24 In veterinary medicine, it has been employed 15,25 with different methodological approaches, including agarose gel, as reported by Zanoni et al 25 In our study, the FSM with Bouin's fixative was easier to reproduce, with adequate results for moderate to highly cellular samples. It showed more highly cellular and aggregated groups as compared to AM, but this finding may be due to the non-cohesive nature of the lymphoid cell population that could easily be diluted into the agarose gel.…”

“…CBs are useful adjuncts to smears for establishing a more definitive cytopathological diagnosis, notably for FNAB samples . With advances in LBC in human medicine, the method has been largely used, since it is a versatile option for the detection of antigens by immunocytochemistry . In veterinary medicine, it has been employed with different methodological approaches, including agarose gel, as reported by Zanoni et al In our study, the FSM with Bouin's fixative was easier to reproduce, with adequate results for moderate to highly cellular samples.…”

Use of Liquid-Based Cytology (LBC) and Cell Blocks from Cell Remnants for Cytologic, Immunohistochemical, and Immunocytochemical Diagnosis of Malignancy

“…The LBC method is currently used for uterine cervical cancer screening [2,3,25,26] with immunocytochemical detection of human papilloma virus (HPV) and positivity of p16 INK4a [13,27]. We [19] and other researchers [18,28,29] have reported that cell block sections from residual SurePath or ThinPrep samples and other LBC samples including aspirates and body fluids are helpful with certain problematic cases, such as metastatic tumor of unknown origin and lesions need to be differential diagnosis. In this study, we could immunohistochemically detect positivity of p16 INK4a in CIS of the cell block specimens.…”

“…LBC is currently recommended for cervical cancer screening [10] with a major advantage of allowing ancillary techniques such as those used in immunocytochemistry and molecular biology [11][12][13][14][15][16][17]. Cell blocks made from cell or tissue remnants of LBC can also be used for immunocytochemistry or immunohistochemistry of specific biomarkers to accurate diagnosis of malignancy [16][17][18][19]. In addition, LBC has currently been used for non-gynecologic cytology [15,17,[19][20][21][22].…”

“…Cell blocks made from cell or tissue remnants of LBC can also be used for immunocytochemistry or immunohistochemistry of specific biomarkers to accurate diagnosis of malignancy [16][17][18][19]. In addition, LBC has currently been used for non-gynecologic cytology [15,17,[19][20][21][22].…”