Application of Luminescent BSA‐Capped CdS Quantum Dots as a Fluorescence Probe for the Detection of Cu<sup>2+</sup>

Han, Song; Liu, Jianli; Zhi-Gang, Gan; Liang, Jiangong; Zhao, Shiyong

doi:10.1002/jccs.200800156

Cited by 11 publications

(4 citation statements)

References 22 publications

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“…NCs with sizes between 0.2 and 3 nm , made of a few to roughly several hundred metal atoms are considered. NCs are characterized by a size comparable with the dimension of the Fermi wavelength of electrons, and they exhibit molecule-like properties. , Several biopolymers such as bovine serum albumin (BSA), − lysozyme, , glucose oxidase (GOx), HRP, glutathione, or DNA have been used to make stable clusters of atoms. The synthesis of NCs stabilized with proteins is usually carried out under extreme conditions in terms of pH or temperature, which causes the denaturation of the proteins.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Antibody-Protected Bimetallic Nanoclusters with Catalytic Properties

et al. 2020

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In this work a novel and facile method for the synthesis of gold-platinum bimetallic nanoclusters (NCs) embedded in the structure of an IgG (Au/Pt NCs-IgG) is presented. Proteins have been widely used as scaffolds for the synthesis of atomic clusters. The harsh conditions usually required during the synthesis imply the denaturation of different proteins and the loss of their biological activity. We propose a strategy for the synthesis of NCs employing IgG as a scaffold performed using physiological conditions in order to maintain unalterable the IgG structure allowing the resulting material bind to Protein G and the antigen. NCs composed of two different types of atoms exhibit higher catalytic activity than monometallic nanoclusters (NC), due to the synergistic effect of two diverse atoms. This peroxidase-like activity and the maintained affinity for its antigen make Au/Pt NCs-IgG a suitable material for its use as a detection antibody in a direct sandwich Enzyme-Linked Immuno Sorbent Assay (ELISA). Use of Au/Pt NCs-IgG as an alternative to IgG tethered to horseradish peroxidase in ELISA boosts the limit of detection (LOD) by 56 times.

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Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Antibody-Protected Bimetallic Nanoclusters with Catalytic Properties

et al. 2020

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“…BSA does not have any affinity for protein G; therefore, any binding to the protein G should be detected. The CdS NCs stabilized using BSA are fluorescent 6 and have catalytic activity toward the oxidation of Amplex Red in the presence of H 2 O 2 (Figure S10). BSA carrying Ag/Pt NCs synthesized by us showed catalytic properties with respect to the oxidation of TMB in the presence of H 2 O 2 (Figure S11).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The spatial isolation of free electrons in NCs provides electronic transitions tunable with size . Several biopolymers like bovine serum albumin (BSA), − lysozyme, , glucose oxidase (GOx), horseradish peroxidase (HRP), glutathione, or DNA have been used as stabilizers for semiconductor and metallic clusters of atoms. The harsh conditions employed during the synthesis of semiconductor and metallic NCs, such as high temperature or extremely basic pH, with these biopolymers as scaffolds cause partial or complete loss of their initial biological properties due to denaturation.…”

Section: Introductionmentioning

confidence: 99%

Antibody-Directed Synthesis of Catalytic Nanoclusters for Bioanalytical Assays

Mora-Sanz

Saá

Briz

et al. 2020

ACS Appl. Mater. Interfaces

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Synthesis of atomic nanoclusters (NCs) using proteins as scaffold has attracted great attention. Usually the synthetic conditions for the synthesis of NCs stabilized with proteins require extreme pHs or temperature.These harsh reaction conditions cause the denaturation of the proteins and end up in the loss of their biological functions. Until now there are no examples of the use of antibodies as NCs stabilisers. In this work we present the first method for the synthesis of catalytic NCs that uses antibodies for the stabilization of NCs. Anti-BSA IgG was used as a model in order to demonstrate that it is possible to use an antibody as scaffold for the synthesis of semiconductor and metallic NCs with catalytic properties. The synthesis of antibodies modified with NCs is carried out under non denaturing conditions, which do not affect the antibody structure. The resulting antibodies still maintain the affinity for target antigens and protein G. The catalytic properties of the anti-BSA IgG modified with NCs can be used to the quantification of BSA in a direct sandwich ELISA.Supporting Information. Experimental section, absorption and fluorescence spectra of CdS NCs-IgG and Ag/Pt NCs-IgG, ICP analysis, optimization experiments, characterization of BSA-stabilized NCs, and size distribution of NCs. The following files are available free of charge.

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“…Uppa et al [45] also prepared Hg 2+ fluorescent probes relying on cysteamine capped CdS QDs modulated Ag + , and this probe shown excellent detection sensitivity. Han and coworkers [46] synthesized BSA functionalized CdS QDs by a stepwise procedure, and they were used as fluorescence sensors for the determination of Cu 2+ . This sensor exhibited good interference and linear responses in the range of 0-80 µM.…”

Section: Qd-based Fluorescence Sensorsmentioning

confidence: 99%

Fluorescent Quantum Dots and Its Composites for Highly Sensitive Detection of Heavy Metal Ions and Pesticide Residues: A Review

et al. 2023

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Quantum dots nanomaterials have attracted extensive interest for fluorescence chemical sensors due their attributes, such as excellent optical characteristics, quantum size effects, interface effects, etc. Moreover, the fluorescence properties of quantum dots can be adjusted by changing their structure, size, morphology, composition, doping, and surface modification. In recent years, quantum dots nanomaterials have been considered the preferred sensing materials for the detection of heavy metal ions and pesticide residues by the interactions between quantum dots and various analytes, showing excellent sensitivity, selectivity, and interference, as well as reducing the cost of equipment compared with traditional measurement methods. In this review, the applications and sensing mechanisms of semiconductor quantum dots and carbon-based quantum dots are comprehensively discussed. The application of semiconductor quantum dots, carbon quantum dots, graphene quantum dots, and their nanocomposites that are utilized as fluorescence sensors are discussed in detailed, and the properties of various quantum dots for heavy metal ion and pesticide residue determination are also presented. The recent advances in and application perspectives regarding quantum dots and their composites are also summarized.

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Application of Luminescent BSA‐Capped CdS Quantum Dots as a Fluorescence Probe for the Detection of Cu²⁺

Cited by 11 publications

References 22 publications

Synthesis and Characterization of Antibody-Protected Bimetallic Nanoclusters with Catalytic Properties

Synthesis and Characterization of Antibody-Protected Bimetallic Nanoclusters with Catalytic Properties

Antibody-Directed Synthesis of Catalytic Nanoclusters for Bioanalytical Assays

Fluorescent Quantum Dots and Its Composites for Highly Sensitive Detection of Heavy Metal Ions and Pesticide Residues: A Review

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Application of Luminescent BSA‐Capped CdS Quantum Dots as a Fluorescence Probe for the Detection of Cu2+

Cited by 11 publications

References 22 publications

Synthesis and Characterization of Antibody-Protected Bimetallic Nanoclusters with Catalytic Properties

Synthesis and Characterization of Antibody-Protected Bimetallic Nanoclusters with Catalytic Properties

Antibody-Directed Synthesis of Catalytic Nanoclusters for Bioanalytical Assays

Fluorescent Quantum Dots and Its Composites for Highly Sensitive Detection of Heavy Metal Ions and Pesticide Residues: A Review

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Application of Luminescent BSA‐Capped CdS Quantum Dots as a Fluorescence Probe for the Detection of Cu²⁺