Application of MALDI-TOF MS for the Identification of Food Borne Bacteria

Abstract

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently emerged as a powerful tool for the routine identification of clinical isolates. MALDI-TOF MS based identification of bacteria has been shown to be more rapid, accurate and cost-efficient than conventional phenotypic techniques or molecular methods. Rapid and reliable identification of food-associated bacteria is also of crucial importance for food processing and product quality. This review is concerned with the applicability of MALDI-TOF MS for routine identification of foodborne bacteria taking the specific requirements of food microbiological laboratories and the food industry into account. The current state of knowledge including recent findings and new approaches are discussed.

Isolation, Identification and Screening of Potential Probiotic Bacteria in Milk from South African Saanen Goats

“…In fact, some species are not easily distinguishable in terms of phenotypic characteristics (Coeuret et al, 2003). Rapid and precise species identification of food-associated bacteria is also of crucial importance for food processing and product quality (Pavlovic et al, 2013). MALDI-TOF based identification technique, has been shown to be rapid, accurate and cost-effective.…”

Beyond the Matrix-Assisted Laser Desorption Ionization (MALDI) Biotyping Workflow: in Search of Microorganism-Specific Tryptic Peptides Enabling Discrimination of Subspecies

“…Protein extraction was performed in accordance with a procedure previously described (18) and lately applied by many research groups (19)(20)(21)(22)(23)(24)(25)(26). For classical biotyping, which uses undigested cell extracts, Salmonella enterica subsp.…”

Advanced Mass Spectrometry

“…The basic principles of operation are shown in Figure 4. Today's TOF mass spectrometers offer excellent sensitivity and high mass range as well as high-speed analysis [34][35][36][37][38][39][40][41]. This has made TOF-MS an essential instrument for food analysis applications-typically with matrix-assisted laser desorption/ionization (MALDI) and ESI ionization-particularly with the development of high-resolution and hybrid instruments [quadrupole time-offlight (QqTOF), ion-trap time-of-flight (IT-TOF), and TOF-TOF configurations].…”

“…Since the advent of the use of MALDI-TOF-MS as a tool for microbial characterization, efforts to increase the taxonomic resolution of the approach have been made. These efforts are one of the most widely covered fields in several review articles in which recent finding and new approaches are discussed [39][40][41][42]. An alternative developed two decades ago is surface enhanced laser desorption/ionization time-of-flight mass spectroscopy (SELDI-TOF-MS).…”