Application of maltitol to improve production of raw starch digesting glucoamylase by Aspergillus niger F-08

Abstract

The effect of the addition of maltitol on the production of raw starch digesting glucoamylase (RSDG) by Aspergillus niger F-08 was studied in the paper. The activity of RSDG formed by Aspergillus niger F-08 was enhanced dramatically by the addition of maltitol to the medium and it was confirmed that maltitol acted as a very efficient inducer for RSDG production. As a result, with the addition of 8 g maltitol in 1 l basal medium at the beginning of the culture, the enzyme activity was enhanced near 8-fold. The synthesis mechanism of RSDG by Aspergillus niger F-08 was also studied on molecular level. It was found that the regulation of RSDG synthesis by Aspergillus niger F-08 occurred at both transcriptional and translational level and the enzyme synthesis was provoked by the addition of maltitol at transcriptional level.

Efficient hydrolysis of raw starch and ethanol fermentation: a novel raw starch-digesting glucoamylase from Penicillium oxalicum

“…The RSDGs are mainly produced by fungi or yeasts, such as Aspergillus niger [ 14 , 15 ], Aspergillus oryza e [ 16 ], Aspergillus awamori [ 17 ], Corticium rolfsii [ 18 ], Penicillium sp. [ 19 , 20 ], and Aureobasidium pullulans [ 21 ].…”

Influence of NH4Br on Solvation Behavior of Polyhydroxy Solutes in Aqueous Solutions at Different Temperatures and Atmospheric Pressure

“…The properties of solutions of polyols and saccharides in mixed aqueous media are of considerable interest in basic research and are applied in food, pharmacy, chemical engineering, crystallization, desalination, etc. − Binary and ternary aqueous solutions of saccharides, inorganic salts, alcohols, and polyols can be used as osmotic agents, and to adjust the water activity and pH in order to reduce the growth of contaminated microorganisms. − Xylitol is broadly used as sweetner, and D-mannitol is used as hyperosmolar solution in the therapy of elevated intracranial pressure in brain trauma. , Polyhydroxy solutes can increase the thermal stability of proteins or reduce the extent of their denaturation by other reagents. Disaccharides are used as cryoprotectants against the destabilizing and degradation of enzymes and drugs during lyophilisation properties.…”

“…2 ) P , by which qualitative information on hydration of a solute could be obtained from the thermal expansion of an aqueous solution. The sign of the left-hand side of the above equation {(∂C p , 2 °/∂P) T < 0, (∂C p , 2 °/∂P) T > 0} provides a direct probe of kosmotropic and chaotropic properties of stabilization or destabilization, respectively.…”

Production of raw cassava starch-degrading enzyme by Penicillium and its use in conversion of raw cassava flour to ethanol

“…[22,23] and Rhizopus sp. [15,26] possess the ability to produce raw starch-degrading enzymes which can act on raw corn starch [11,[21][22][23] and raw potato starch [8] effectively. Production of a raw sago starchdegrading enzyme by the fungus Acremonium sp.…”