Application of Mapping and Pinboard Mapping for Observation of Corn Plantation Root at Inceptisol Soil, Tlogomas, Malang

Susilawati, Siti Azizah

doi:10.23917/forgeo.v18i1.601

Cited by 4 publications

(14 citation statements)

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“…(Table 3). This result was in agreement with Susilawati et al (2011) which stated that the X sperm was bigger than the Y sperm. In cattle, the average length of the sperm head was 8.75 µm.…”

Section: Resultssupporting

confidence: 91%

“…This difference occurred because the amount of Y sperm went through the 10% BSA column for a certain period was less than the amount of X sperm penetrating the 5% BSA column. The smaller and lighter Y sperm head made the Y sperm movement much faster than that of X sperm (Susilawati 2011). The viability of the X sperm was 79.6%, and that of the Y sperm was 82.66 %.…”

Section: Resultsmentioning

confidence: 96%

“…Plasma membrane integrity of sperm is correlated with the motility and viability of the sperm. According to Susilawati (2011), if a sperm with an intact membrane is placed on a hypoosmotic medium, it will increase the water content in its cytoplasm to keep the balance of the solutions inside and outside the cell, thereby causing the sperm tail to curl, hence, indicating a motile sperm. (Table 3).…”

Section: Resultsmentioning

confidence: 99%

“…Arifiantini et al (2011) discovered that the total abnormality found in the sperm of swamp buffalo was 31.86%; while, the primary abnormality constitutes 9.93% of the total sperm observed. Susilawati (2011) suggested that the cattle sperm abnormality of 20% could reduce sperm fertility. Similarly, in sheep, there was a positive correlation between the morphology and the motility of the ejaculated semen, indicating that sperm abnormality affected male fertility (Susilawati 2011).…”

Section: Resultsmentioning

confidence: 99%

“…Fertilizing an oocyte, an X sperm will form a female embryo, while a Y sperm will form a male embryo. The Y sperm possesses an SRY (sex-determining region Y) gene that functions in controlling the formation testes in male animals and the gene is not found in X sperm (Susilawati 2011). Macroscopic analysis indicated that the semen of spotted buffalo has a volume ranging between 0.3-3.8 mL; white and creamy in color with a motility of 68-79% (Battosamma 1985;Tappa 2006).…”

Section: Introductionmentioning

confidence: 99%

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Morphometry and abnormality evaluation of sex-sorted sperm of spotted buffalo (Tedong bonga)

2017

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Abstract. Kaiin EM, Gunawan M, Maulana T. 2017. Morphometry and abnormality evaluation of sex-sorted sperm of spotted buffalo (Tedong bonga). Nusantara Bioscience 9: 175-180. This research aimed to determine the morphometry and the abnormality of the spotted buffallo (Tedong bonga) sperm after the separation of X and Y-chromosome-bearing sperms. Semen was collected from the spotted buffalo bull by using an artificial vagina.The semen quality was evaluated macro-and microscopically. Semen that met the required standard were subjected to separation by using Bovine Serum Albumin (BSA) 5% and 10% column method. The quality of sex-sorted sperm was evaluated microscopically, including motility, concentration, viability, abnormality and intact plasma membrane. Smear preparation using Eosin-Nigrosin dyes was made for sperm morphometry and abnormality evaluation.The evaluation of sperm morphometry and abnormality was conducted on 200 sperm cells for each observation and repeated in triplicates of observation, by using Axiovision Imager Z microscope at objective and ocular magnifications 40x and 10x, respectively.The morphological parameters observed were including the length and the width of head sperm and the head area of sperm X or Y. The abnormality parameter consisted of primary and secondary abnormalities. The data were analyzed descriptively. The results showed that the head size of the Y sperm of spotted buffalo (27.16 µm 2 ) was smaller than that of the X sperm (29.86 µm 2 ) with the abnormality of the Y sperm (7.85%) was also lower than that of the X sperm (9.7%).

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Morphometry and abnormality evaluation of sex-sorted sperm of spotted buffalo (Tedong bonga)

2017

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Correlations between age of Bali bull and semen production at National Artificial Insemination Center, Singosari - Indonesia

Nugraha

Herwijanti²,

Novianti

et al. 2019

J. Indonesian Trop. Anim. Agric.

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The aim of this research was to analyze the correlation of age of Bali bull to the body weight and semen qualities. The materials were 50 Balibulls data collected from 2012 to 2018. Therecordeddatawere consisted ofsemen productions (n = 1304) and body weight (n = 1544). The observation was conducted to analysis the interrelationship between the Bali bulls age and body weight (BW), to the semen volume (SV), individual motility (IM) and sperm concentration (SC). The obtained data was analyzed with One-Way ANOVA analysisby using SPSS 24 and followed with Duncan’s Multiple Range Test to determine any significant differences. The correlation between Bali bulls age (the independent variable) and semen quality (the dependent variable) was computed by using Pearson correlation method and presented in linear regression models. The results showed that the Bali bulls agesignificantly affect (P<0.05) the BW from the age of 1 to 10 years old. The age also showed a significant effect (P<0.05) on the semen qualities. The age correlated significantly (P<0.05) with BW, SV, IM, SC were 0.658, 0.386, 0.134 and 0.086, respectively. Inconclusion,the age of Bali bull could be used as an indicator todetermineits BWand semenqualities.

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Pengaruh perbedaan waktu inseminasi buatan terhadap keberhasilan kebuntingan sapi Brahman Cross

annashru¹,

Ihsan²,

Yekti³

et al. 2017

JIIP

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ABSTRAK :Tujuan penelitian ini adalah mengetahui pengaruh perbedaan waktu IB terhadap keberhasilan kebuntingan sapi Brahman Cross ditinjau dari waktu dan Non Return Rate (NRR)1. Materi dalam penelitian ini adalah sapi betina Brahman Cross berjumlah 75 ekor, 35 ekor di IB dengan interval 8-12 jam dan 40 ekor di IB dengan interval 0-4 jam. Metode yang digunakan dalam penelitian adalah percobaan lapang. Pemilihan sampel ternak menggunakan Purpose Sampling dengan kriteria sapi betina dewasa bobot badan > 250, umur 1,5 -2 tahun, sehat, dan bebas dari gangguan penyakit dan minimal memiliki kondisi berahi. Bahan dalam penelitian ini adalah semen beku sapi Brahman yang diperoleh dari Balai Inseminasi Buatan (BIB) Lembang, Bandung Jawa Barat dengan motilitas 35-40%. Variabel dalam penelitian ini adalah kebuntingan sapi dan munculnya tanda-tanda berahi. Data yang diperoleh dianalisis secara deskriptif analitik dan uji chi-square. Data pendukung berupa pakan dan nilai dari heat detector (HD). Hasil penelitian menunjukkan bahwa sapi Brahman Cross yang di IB pada interval 0-4 jam memiliki CR berdasarkan NRR 1 lebih tinggi, yakni 70% dibandingkan dengan yang di IB pada interval 8-12 jam memiliki nilai CR 37,14%. Berdasarkan uji chi-square dinyatakan bahwa perbedaan waktu IB pada sapi Brahman Cross adalah p<0,05 yang artinya perbedaan waktu IB pada sapi Brahman Cross berpengaruh terhadap keberhasilan kebuntingan. Disarankan IB pada 0-4 jam setelah terdapat tanda-tanda berahi dan dilakukannya pengamatan NRR2 hingga NRR3 dan Pemeriksaan kebuntingan (PKB) untuk mengetahui keberhasilan kebuntingan pada sapi Brahman Cross secara sempurna.Kata kunci : Inseminasi buatan, sapi brahman cross, siklus estrus, kebuntingan ABSTRACT : The purpose of this research was to evaluated the difference of AI time towards Brahman Cross pregnancy rate determined by time and Non-Return Rate (NRR). The subjects in this research are 75 female Brahman Cross. Methods that were used during this research were field testing. Selection method of the sample livestock used is Purpose Sampling with criteria: Heifer with weight >250kg, aged 1,5 -2 years, healthy, and free from any disease, and at least have mating urge. Materials used during this research were frozen cow semen that acquired from Balai Inseminasi Buatan (BIB) Lembang, Bandung, West Java with 35-40% motility. The variables were Cow's pregnancy and mating urge's symptoms. Data acquired during this research were analyzed descriptively and chi-square tested. Supportive data were food and value from heat detector (HD). Research result shows that Brahman Cross species which AI within 0-4 hours interval have higher CR according to NRR1, which is 70%. Compared with the other breed that AI within 8-12 hour interval, which had 37,14% CR value.According to chi-square test concluded that difference of AI time off is affecting the pregnancy rate. It is advised that AI is executed during 0-4 hour interval after showing mating symptoms and further review about the difference between AI execution time of Brahman ...

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Application of Mapping and Pinboard Mapping for Observation of Corn Plantation Root at Inceptisol Soil, Tlogomas, Malang

Cited by 4 publications

References 0 publications

Morphometry and abnormality evaluation of sex-sorted sperm of spotted buffalo (Tedong bonga)

Morphometry and abnormality evaluation of sex-sorted sperm of spotted buffalo (Tedong bonga)

Correlations between age of Bali bull and semen production at National Artificial Insemination Center, Singosari - Indonesia

Pengaruh perbedaan waktu inseminasi buatan terhadap keberhasilan kebuntingan sapi Brahman Cross

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