This study accounted for the importance of daily expansion flow data in compensating for insufficient flow data in a watershed. In particular, the 8-day interval flow measurement data (intermittent monitoring data) could cause uncertainty in the high- or low-flow conditions that have been used to estimate the flow duration curve (FDC) and the load duration curve (LDC) used in Total Maximum Daily Load (TMDL) evaluation in Korea. Thus, this study developed a method to expand the 8-day interval flow data (missing data) to daily flow data in order to evaluate the Total Maximum Daily Load (TMDL) appropriately in a watershed. We employed the machine learning technique (the gradient descent method provided by the Google TensorFlow package) to develop a regression for expanding the 8-day interval flow data. The method was applied in the Nakdong River basin located in Korea to collect the 8-day interval and daily flow data from a number of gauging stations. The results of the expanded daily flow were evaluated through the RMSE, MAE, IOA, and NSE, and the valid expanded daily flow data were obtained for the 29 TMDL gauging stations (IOA 0.84~0.99, NSE −0.18~0.99). A good performance in the creation of daily flow data (continuous data) from the 8-day interval flow data (intermittent data) was shown using the proposed method. In addition, the Web GIS-based pollutant load assessment system was developed to evaluate the TMDL; it included the daily data expansion method and provided the pollution load characteristics objectively and intuitively. This system will help decision makers, such as environmental regulators, researchers, and the general public, and support their decision making for pollution source management with accessible and efficient tools for understanding and addressing water quality issues.