Application of miniaturized physiological tests in numerical classification and identification of some bacilli.

Kämpfer, Peter

doi:10.2323/jgam.37.225

Cited by 21 publications

(17 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This value was lower than the one presented by K~impfer [32] (75% SSM ) or by Logan and Berkeley [34] (82% with the S~ coefficient).…”

contrasting

confidence: 46%

“…The same three groups were recognized with the Sj, the SSM and the S G coefficients at a similarity level of 57%, 71% and 70% respectively. Because of the similarity of the three classifications and because several authors [20,32,33] have presented their numerical studies with the SSM coefficient, only the UPGMA-SsM analysis is described here to facilitate comparison with previous results. In order to compare more efficiently the results of the phenogram with the genomic analysis and the bacterial distribution on evergreen oak leaves described below, we defined a total of five clusters (containing two or more …”

Section: Phenotypic Classificationmentioning

confidence: 99%

“…Yet, the five isolates contained in cluster 3a of the phenogram belonged to the genomic subspecies 2a and isolates from cluster 4 and subcluster 3b belonged to genomic subspecies 2b. To our knowledge, only K~impfer's work [32] described two distinct B. pumilus clusters by numerical analysis: cluster 21 which included the DSM27 astrain, and cluster 11 which contained a few strains and presented only 74% of similarity (SsM coefficient) with cluster 21. Our B. pumilus isolates would probably be close to cluster 11 and none of them would then belong to the distant cluster 21.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Occurrence of Bacillus species on evergreen oak leaves

Brunel

Périssol

Fernández

et al. 1994

FEMS Microbiology Ecology

View full text Add to dashboard Cite

Thirty-five spore-forming bacilli were isolated from evergreen oak leaves at four stages (one-year-old and two-year-old leaves, upper layer and underlayer litter leaves) and over the four seasons within one year. These isolates, plus five reference strains, were characterized morphologically and physiologically by a total of 100 tests and genetically by DNA/DNA hybridization. Phenotypic similarities of all strains were determined by numerical taxonomy, with the unweighted average linkage (UPGMA) algorithm and simple matching (SsM) and Jaccard (S j) coefficients used as measures for similarity. Three groups (A to C) were defined at a similarity level of 71% (SsM) or 57% (S j). They contained leaf isolates phenotypically related to Bacillus cereus, B. pumilus and B. circulans species respectively. The majority of the leaf isolates were assigned to B. cereus (34%) and B. pumilus (63%). DNA/DNA hybridization also discriminated three groups (genomic groups 1, 2 and unclassified strains) which presented a good correlation with the numerical analysis. Yet, DNA/DNA hybridization grouping revealed a higher degree of discrimination by defining four subgroups (la, lb, 2a and 2b). Genomic subgroup la contained leaf isolates belonging to the B. cereus species; isolates from genomic subgroup lb belonged to B. mycoides species and isolates from genomic subgroup 2a belonged to B. pumilus species. Subgroup 2b consisted of a new genomic subspecies of B. pumilus which exhibited a degree of homology ranging from 53 to 64% with the B. pumilus type strain and a coefficient of divergence (ATm) ranging from 5.5 to 7°C. The different genomic groups presented different substrate metabolism capacities and a different spatial distribution on evergreen oak leaves. B. cereus strains (group 1) were predominantly located on litter leaves whereas B. pumilus strains (group 2) were found on the phyllosphere. In contrast with group 1, group 2 was able to metabolize some sugars and pectin, while group 1 isolates were able to hydrolyze starch and glycogen. Thus, our hypothesis is that group 1 succeeds group 2 when the leaves are littered.

show abstract

“…This value was lower than the one presented by K~impfer [32] (75% SSM ) or by Logan and Berkeley [34] (82% with the S~ coefficient).…”

contrasting

confidence: 46%

Section: Phenotypic Classificationmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Occurrence of Bacillus species on evergreen oak leaves

Brunel

Périssol

Fernández

et al. 1994

FEMS Microbiology Ecology

View full text Add to dashboard Cite

show abstract

“…Despite the heterogeneity, the isolate Rt 10 could be distinguished from all 18 B.firmus-lentus isolates investigated previously (Kiimpfer 1991) by the utilization of D-galacturonate, D-lyxose, L-lyxose, D-tagatose, tartronate, diaminopimelate, and phenylglycine and the hydrolysis of p-nitrophenyl-N-acetyl-a-D-glucosaminide and L-proline-p-nitroanilide. The API test system showed no closer relationship to any species in the data base ( Table 2).…”

Section: G+c Contentmentioning

confidence: 79%

Bacillus oleroniussp.nov., a member of the hindgut flora of the termiteReticulitermes santonensis(Feytaud)

Kuhnigk¹,

Borst²,

Breunig³

et al. 1995

Can. J. Microbiol.

Self Cite

View full text Add to dashboard Cite

A new rod-shaped endospore-forming bacterium is described, which was isolated from the hindgut of the termite Reticulitermes santonensis (Feytaud). The isolate stains Gram negative and its DNA has a guanine-plus-cytosine content of 35 mol%. Despite the Gram-staining reaction, both biochemical and physiological features place the isolate in the genus Bacillus and indicate a phenotypic resemblance to the Bacillus firmus-lentus group of species. On the basis of comparative 16S rRNA analysis and some phenotypic features the isolate clearly represents a new species for which the name Bacillus oleronius is proposed. The type strain is Bacillus oleronius Rt 10 (DSM 9356).

show abstract

“…Apart from a few classical biochemical tests (Table 8), we chose carbon substrate utilization tests and qualitative enzyme tests using chromogenic substrates, which can be used to differentiate various Gram-negative nonfermentative bacteria [28,30], in addition to Grampositive bacteria such as bacilli [29] or the heterogeneous groups of coryneforms and nocardioforms [31]. The original set of tests [28] used in this study has been successfully applied elsewhere [32,40].…”

Section: Identification Results and Bacterial Activitiesmentioning

confidence: 99%

Characterization of chemoheterotrophic bacteria associated with the in situ bioremediation of a waste-oil contaminated site

et al. 1993

View full text Add to dashboard Cite

In the course of an in situ bioremediation, different hydrologically controllable test plots were installed on the ground of a waste-oil contaminated site, and continuously injected with nutrient solution and the electron acceptors NO3 (-), O2, and H2O2. In a two-year period, groundwater samples obtained from different recovery wells within these field plots, in addition to subsoil samples, were monitored for several chemical and microbiological parameters. The removal of hydrocarbons observed in the water samples could not unambiguously be attributed to biodegradation, and was probably caused by groundwater treatment measures. However, chemical (gaschromatographic) and microbiological data from the subsoil samples indicated a biological degradation of pollutants. Analysis of the groundwater samples of the different test plots revealed only minor quantitative differences. With time, only a slight increase in bacterial numbers on different media, including hydrocarbon-agar, was observed. In general, chemical and microbiological analyses of groundwater samples cannot replace analyses of subsoil samples for a sufficient documentation of in situ remediation processes in subsoil. From the groundwater and subsoil samples, 3,446 pure cultures, obtained from R2A agar, were characterized morphologically and physiologically, and identified in order to study the culturable bacterial communities. Several qualitative differences in composition and diversity of the bacterial communities among the test plots were observed. More than 70 different species or taxonomic groups (most of them known as hydrocarbon degrading taxa) could be identified from the groundwater samples; these were mainly the Gram-negative genera Acinetobacter, Alcaligenes, Comamonas, Hydrogenophaga, Pseudomonas, Flavobacterium/Flexibacter/Cytophaga, and others. A high proportion of Gram-positive organisms (42.5%), belonging to Bacillus and the various genera of coryneform and nocardioform organisms, were isolated from the subsoil samples.

show abstract

Application of miniaturized physiological tests in numerical classification and identification of some bacilli.

Cited by 21 publications

References 38 publications

Occurrence of Bacillus species on evergreen oak leaves

Occurrence of Bacillus species on evergreen oak leaves

Bacillus oleroniussp.nov., a member of the hindgut flora of the termiteReticulitermes santonensis(Feytaud)

Characterization of chemoheterotrophic bacteria associated with the in situ bioremediation of a waste-oil contaminated site

Contact Info

Product

Resources

About