Application of modified Arxula adeninivorans yeast cells in an online biosensor for the detection of estrogenic compounds in wastewater samples

Pham, Ha Thi Minh; Kunath, Kirstin; Gehrmann, Linda; Giersberg, Martin; Tuerk, Jochen; Uhlig, Steffen; Hanke, Gerold; Simon, Kirsten; Baronian, Keith; Kunze, Gotthard

doi:10.1016/j.snb.2013.05.065

Cited by 21 publications

(11 citation statements)

References 26 publications

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“…Other methods that use biosensors to detect 17β‐estradiol have shown lower detection limits at nanomolar levels, that is, 0.1853 and 0.036 54. However, for the GC/GO/CdTe electrode, high sensitivity, good reproducibility and simple instrumentation, preparation and analytical procedure are important advantages.…”

Section: Resultsmentioning

confidence: 99%

Graphene Nanosheets and Quantum Dots: A Smart Material for Electrochemical Applications

Cincotto

Moraes

Machado

2014

Chemistry A European J

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A novel material for the electrochemical determination of endocrine disruptors using a composite based on graphene oxide modified with cadmium telluride quantum dots has been evaluated. The morphology, structure and electrochemical performance of the composite electrodes were characterised by transmission electron microscopy, dynamic light scattering, UV-visible absorption spectra, fluorescence spectra, Raman spectra and cyclic voltammetry. The dynamic light scattering, transmission electronic microscopy and spectrophotometric measurements all showed good distribution of the quantum dots with a small particle size. The electrochemical measurements demonstrated the high performance of the composite response in the presence of a light source. Differential pulse voltammetry allowed the development of a method to determine 17β-estradiol levels in the range from 0.2 to 4.0 μmol L(-1) with a detection limit of 2.8 nmol L(-1) (0.76 μg L(-1)).

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Section: Resultsmentioning

confidence: 99%

Graphene Nanosheets and Quantum Dots: A Smart Material for Electrochemical Applications

Cincotto

Moraes

Machado

2014

Chemistry A European J

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“…Currently the most popular determination methods use laboratory analytical devices. There are a number of yeastbased assays for the detection of hormones [27][28][29][30][31][32][33][34], but assays for particular or total pharmaceuticals are lacking. Although conventional analytical methods are sensitive, they do not reflect the responses of organisms.…”

Section: Discussionmentioning

confidence: 99%

“…Other widely used methods are based on biosensors which focus most on the determination of hormones (estrogenic, androgenic, gestagenic molecules) for example the YES-assay [27], E-Screen assay [28], A-YES assay [29], nAES assay [30], EstraMonitor apparatus [31,32], A-YAS [33] and recombinant cell bioassays [34]. There are several bacterial and yeast-based assays for pharmaceutical screening [35,36] which are used for detecting genotoxicity.…”

Section: Introductionmentioning

confidence: 99%

The determination of pharmaceuticals in wastewater using a recombinant Arxula adeninivorans whole cell biosensor

Pham¹,

Giersberg²,

Gehrmann³

et al. 2015

Sensors and Actuators B: Chemical

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“…The effect of a wide range of substances as well as the antagonistic properties of some EDCs was then investigated. Because a potential application of the assay is in water analysis, different samples from a wastewater treatment plant (WWTP) were assayed without extraction of the compounds to demonstrate the utility of the system and the robustness of A. adeninivorans [37,25]. …”

Section: Introductionmentioning

confidence: 99%

Development of a recombinant Arxula adeninivorans cell bioassay for the detection of molecules with progesterone activity in wastewater

et al. 2015

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This study describes the development of a bioassay to detect the presence of progesterone and progesterone-like molecules in wastewater samples. The basis of the bioassay is the integration of the human progesterone receptor gene into the yeast Arxula adeninivorans for the constitutive synthesis of the receptor. After incubation, binding of the analyte to the receptor induces the production of a reporter protein. Two reporter proteins were compared for detection parameters such as half-maximal activity (EC50), limit of detection (LoD) and limit of quantification (LoQ). When the extracellular phytase K was used, an EC50 value of 155 ng L(-1) and a LoD of 27 ng L(-1) progesterone were obtained after 4 h incubation, while use of the fluorescent dsRED as the reporter protein, resulted in an EC50 of 320 ng L(-1) and a LoD of 65 ng L(-1) after 20 h incubation. Use of phytase K as the reporter protein offers decreased incubation time and increased sensitivity; however the dsRED reporter system is less labor-intensive. Additionally, the affinity of known agonists and antagonists of the human progesterone receptor was determined. The utility of this bioassay was confirmed by measuring total progesterone equivalent concentration of samples from a wastewater treatment plant. The A. adeninivorans-based transactivation assay was able to measure concentrations of about 311 ng L(-1) in the influent stream but could not detect progesterone activity in effluent. One key feature of the assay is the robustness of A. adeninivorans, which allows sample measurement without any sample preparation.

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Application of modified Arxula adeninivorans yeast cells in an online biosensor for the detection of estrogenic compounds in wastewater samples

Cited by 21 publications

References 26 publications

Graphene Nanosheets and Quantum Dots: A Smart Material for Electrochemical Applications

Graphene Nanosheets and Quantum Dots: A Smart Material for Electrochemical Applications

The determination of pharmaceuticals in wastewater using a recombinant Arxula adeninivorans whole cell biosensor

Development of a recombinant Arxula adeninivorans cell bioassay for the detection of molecules with progesterone activity in wastewater

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