Application of monofluorophosphate/alkaline phosphatase system in flow injection analysis

Rozum, Beata; Koncki, Robert

doi:10.1016/j.talanta.2008.04.005

Cited by 8 publications

(2 citation statements)

References 44 publications

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“…Finally, ALP bioassays can be adapted for quite selective indirect determinations of enzyme cofactor and inhibitors. 13 The photometric device presented in this work could be easily applied for all of these bioanalytical schemes as well as for the detection of a large numberd of other enzymes catalyzing the hydrolysis of NP esters, like those aforementioned in the Introduction to this note.…”

Section: Discussionmentioning

confidence: 99%

UV-PEDD photometry dedicated for bioanalytical uses

Tymecki

Brodacka

Rozum

et al. 2009

Analyst

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Detection of p-nitrophenol, a product of several enzymatic reactions, is useful and important in many fields of modern bioanalytics. Here, we have developed and applied a complete photometer dedicated for such measurements that is extremely economic and easily customized for specific experimental setups. The instrument consists only of paired emitter-detector diodes (PEDDs) coupled with an extremely simple and low-cost signal transduction system. The experimental data confirmed that this photometric system adapted for cuvette as well as flow measurements can be applied for fast and reproducible determination of alkaline phosphatase (ALP) activity.

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Section: Discussionmentioning

confidence: 99%

UV-PEDD photometry dedicated for bioanalytical uses

Tymecki

Brodacka

Rozum

et al. 2009

Analyst

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show abstract

“…Polymeric membrane ISEs have been used for sensitive detection of enzymes such as horseradish peroxidase (Nagy et al, 1973), alkaline phosphatase (Rozum and Koncki, 2008), butyrylcholinesterase Qin, 2009a, 2009b) and urease (Koncki, 2007). In those enzymatic assays, the detectable ions are restricted to singly charged reagents.…”

Section: Introductionmentioning

confidence: 99%