Application of  Morphological  Analysis and DNA Barcode in Determination of The Porcelain Crab Species (Pisidia sp.) from The Tunda Island, Banten

Triandiza, Teddy; Maddupa, Hawis

doi:10.30862/jsai-fpik-unipa.2018.vol.2.no.2.51

Cited by 10 publications

(7 citation statements)

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“…Ident 99.85%. According to Triandiza and Madduppa (2018), the Max score, and total score with the same value, Query cover nearly reached 100%, Evalue was close to 0, and Per. Ident approached 100% in each database, denoting the most similar GenBank sequences (Table 2).…”

Section: Molecular Analysismentioning

confidence: 86%

The Analysis of Morphological and Genetic Characteristics of Yellowstripe Scad from Muara Baru Modern Fish Market in North Jakarta

Sawalman

Madduppa

2020

JIPK

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HighlightIdentification and morphological analysis of Selaroides leptolepis fish.Molecular analysis using the DNA Barcoding method with the COI gene (mitochondrial cytochrome oxidase subunit I).The number of base pairs in S. leptolepis from the sequencing results was 675bp.The morphological and molecular similarities of Selaroides leptolepis fishAbstractYellowstripe scad is one of the commercially important fish from the Carangidae family, which is marketed at Muara Baru Modern Fish Market, North Jakarta. In- formation regarding the presence of Selaroides leptolepis fish in the waters allows for effective conservation, and management of marine resources. A morphological identification of fish species is still considered inaccurate, so the molecular anal- ysis is necessary. This study aims to identify commercially important fish species deriving from Muara Baru Modern Fish Market, North Jakarta, employing mor- phological, and molecular analysis. A total of 30 specimens were collected from the field. The morphological analysis utilized a visual identification method, and morphometric measurement, while molecular analysis with DNA barcoding em- ployed the mitochondrial cytochrome oxidase subunit I (COI) gene. The results of the morphological analysis indicated that the fish species were yellowstripe scad (S. leptolepis). It also followed the results of molecular analysis of DNA barcoding that the fish was a S. leptolepis species. Therefore, the combination of morphological and genetic analysis has succeeded in identifying the fish species of S. leptolepis.

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Section: Molecular Analysismentioning

confidence: 86%

The Analysis of Morphological and Genetic Characteristics of Yellowstripe Scad from Muara Baru Modern Fish Market in North Jakarta

Sawalman

Madduppa

2020

JIPK

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“…We compared our sequences with those from NCBI GenBank and BOLD database and discovered that the 23 mudskipper samples in this study belonged to two distinct species, P. kalolo (20 samples) and P. argentilineatus (3 samples). According to Triandiza and Maduppa (2018), the most similar GenBank sequence is characterized by the same maximum and total score, query coverage close to 100%, E value close to 0, and percentage of identity or similarity close to 100%. Based on that criterion, all mudskipper samples in this study showed greater similarity to the sequence of both species in GenBank.…”

Section: Discussionmentioning

confidence: 99%

Genetic Identification of Two Mudskipper Species (Oxudercidae: <i>Periophthalmus</i>) from Kulon Progo, Special Region of Yogyakarta, Indonesia

Febriyanti,

Aji,

Priyono

et al. 2023

J.Tropical Biodiversity Biotechnology

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Mudskippers are commonly cryptic species, making identification based solely on morphological characteristics challenging. This study used the DNA barcoding method to identify mudskipper species based on the COI mitochondrial gene. The analysis revealed two distinct species, P. kalolo (20 samples) and P. argentilineatus (3 samples) with high GC contents ranging from 42.94-45.2%. The genetic divergence analysis of P. kalolo showed that they divided into two clades, while P. argentilineatus is divided into three clades with two of the clades (C and D) still conspecific groups, and those two clades with clade E exhibit a genetic distance greater than 3.5%, suggesting the presence of cryptic species. These findings provide valuable insights into the intraspecies genetic diversity of mudskippers in Indonesia, which could have essential implications for conservation efforts and highlight the potential of DNA barcoding as a powerful tool for the identification of cryptic species. Further research combining molecular and morphological identification could lead to a more comprehensive understanding of species identification and help address the challenges posed by cryptic species.

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“…The results of the BLAST analysis provide information about the percentage of chain length used in the database search process. The highest similarity in the BLAST analysis can be characterized by the query cover value and the percentage identity value which is close to 100% [24]. The results of the nucleotide matching of the Meulaboh1 sample using BLAST indicated that the sample was identified as a hammerhead shark.…”

Section: Molecular Identification Of Hammerhead Sharkmentioning

confidence: 93%

“…The low genetic distance value indicates that the two populations have a close relationship. On the other hand, a large genetic distance value indicates that the two populations are distantly related [24], [36]. The results of the calculation of genetic distance are presented in the form of matrix data which is then used for analysis of kinship between individuals based on kinship.…”

Section: Genetic Distancesmentioning

confidence: 99%

Relationship analysis of Scalloped Hammerhead (Sphyrna lewini) from West Aceh Waters using molecular genetics approach

Bahri

Hikmah²,

Fadli³

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Hammerhead sharks are particularly vulnerable to fishing because of their slow growth rate and reproductive constraints. This type of sharks needs special attention due to its conservation status. This research was aimed to describe the relationships of the hammerhead sharks from West Aceh waters with the hammerhead sharks from other places. Samples of the shark were collected from Ujong Baroh Fish Port, West Aceh. The samples were analysed at the Laboratory of Department of Marine Science and Fisheries, Syiah Kuala University. The laboratory activity was started with extraction of DNA enhanced by Polymerase Chain Reaction method and visualized by an electrolytical. Analysis using BLAST showed that the first sample obtained 94%-95% cover query value and produced a 683 base pair length while the second sample obtained 98% per sample for a cover query value and produced a 658 base pair length. The average nucleotide values of the hammerhead shark were T=32.8, C=26.6, A=25.7 and G=14.9. The phylogenetic tree shows that the hammerhead sharks from the study area were more similar to the hammerhead sharks from Australia and Malaysia. Overall, based on the nucleotide composition, phylogenetic tree and genetic distance, the hammerhead sharks from the study area are unique compared to the populations from the other places.

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Application of Morphological Analysis and DNA Barcode in Determination of The Porcelain Crab Species (Pisidia sp.) from The Tunda Island, Banten

Cited by 10 publications

References 0 publications

The Analysis of Morphological and Genetic Characteristics of Yellowstripe Scad from Muara Baru Modern Fish Market in North Jakarta

The Analysis of Morphological and Genetic Characteristics of Yellowstripe Scad from Muara Baru Modern Fish Market in North Jakarta

Genetic Identification of Two Mudskipper Species (Oxudercidae: <i>Periophthalmus</i>) from Kulon Progo, Special Region of Yogyakarta, Indonesia

Relationship analysis of Scalloped Hammerhead (Sphyrna lewini) from West Aceh Waters using molecular genetics approach

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