Application of multi-residue analytical method for determination of 496 pesticides in frozen gyoza dumplings by GC-MS and LC-MS

Abstract

The applicability of multi-residue analytical method for 496 pesticides (including metabolites) in frozen gyoza dumplings was verified. This method involved extraction using aqueous acetonitrile (around 70% of acetonitrile content) followed by cleanup using octadecylsilyl (ODS) and primary secondary amine (PSA) mini-column solid-phase extraction (SPE). The target compounds were determined by gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS). Validation tests were performed on frozen gyoza dumplings fortified at 0.01 and 0.10 mg/g in accordance with the guideline issued by the Japanese Ministry of Health, Labour and Welfare. Among the 496 pesticides tested, 378 pesticides were found to conform to the guideline when the solvent standard was used, and 432 pesticides conformed when the matrix-matched standard was used, respectively. The limits of quantitation (S/NՆ10) were set at 0.01 mg/g for all, except 20 of the pesticides that were tested. Although no significant matrix effects were observed for most of the pesticides, the use of the matrix-matched standard was preferable to the solvent standard for accurate quantitation. The method was applied to 11 commercial samples, and 14 different pesticides were detected from 8 samples at concentrations ranging from trace levels (Ͻ0.01 mg/g) to 0.11 mg/g.

Evaluation of pesticide residue analysis of dieldrin in soil using a high resolution gas chromatograph/mass spectrometer (HR-GC/MS)

“…이러한 난분해성 물질의 농경지 토양에서 반감기는 5년에서 20년 정도이고, 이후 서서히 감소하는 것으로 알려져 있다. 일본의 경우 Gas Chromatography/Mass Spectrometry (GC-MS) 법과 Liquid Chromatography/Mass Spectrometry (LC-MS) 분석법을 이용하여 500종류의 농약 성분을 분석하고 있 다 (Matsuoka et al, 2011 (Song et al, 2008…”

Effect of Sampling Size on the Determination of Accurate Pesticide Residue Levels in Japanese Agricultural Commodities

“…In January 2008, at least 10 consumers in Japan became sick after ingesting frozen dumplings produced in China that were contaminated with an organophosphate insecticide, methamidophos. , In consideration of the increasing consumer concern for food safety, the Food Safety Commission of Japan (FSCJ) publicized an acute reference dose (ARfD) of 0.003 mg/kg body weight (bw) per day for methamidophos as official information on May 1, 2008 . Subsequently, the FSCJ publicized an ARfD of 0.1 mg/kg bw per day for acetamiprid on August 29, 2008 .…”