Application of Nanodiamonds in Biomolecular Mass Spectrometry

Abstract

The combination of nanodiamond (ND) with biomolecular mass spectrometry (MS) makes rapid, sensitive detection of biopolymers from complex biosamples feasible. Due to its chemical inertness, optical transparency and biocompatibility, the advantage of NDs in MS study is unique. Furthermore, functionalization on the surfaces of NDs expands their application in the fields of proteomics and genomics for specific requirements greatly. This review presents methods of MS analysis based on solid phase extraction and elution on NDs and different application examples including peptide, protein, DNA, glycan and others. Owing to the quick development of nanotechnology, surface chemistry, new MS methods and the intense interest in proteomics and genomics, a huge increase of their applications in biomolecular MS analysis in the near future can be predicted.

Detonation Nanodiamonds for Rapid Detection of Clinical Isolates of Mycobacterium tuberculosis Complex in Broth Culture Media

“…It was speculated that the low concentrations of these potential biomarkers in the cultured media led to the weak intensity of the peaks. Many findings report the NDs could be used to concentrate proteins from liquid media and elevate the total numbers of proteins for MALDI-TOF MS analysis . In this study, 5 and 50 nm of DNDs were used to concentrate the proteins from MGIT broth.…”

“…Many findings report the NDs could be used to concentrate proteins from liquid media and elevate the total numbers of proteins for MALDI-TOF MS analysis. 25 In this study, 5 and 50 nm of DNDs were used to concentrate the proteins from MGIT broth. The intensities of the peaks were enhanced about a factor of 50 as compared with those of the peaks without 5 nm DNDs treatment.…”

“…Nanodiamonds (NDs), due to their chemical inertness, hardness, optical transparency, and biocompatibility, have been applied in analytical chemistry, biology, catalysis, spectroscopy, materials, electronic applications, and quantum computing . A particular type of ultrananocrystalline material called detonation nanodiamonds (DNDs) with the characteristic size of 4–5 nm were produced by detonation of carbon-containing explosives in the former USSR in the 1960s and show good extraction performance in mass analysis. , The combination of high-pressure high-temperature (HPHT) NDs with TOF mass spectrometry can also effectively detect proteins, biopolymers, DNA oligonucleotides, and multiphosphorylated peptides from complex biosamples feasible . Therefore, ND MALDI-TOF MS is a good platform to detect potential biomarkers under critical environments (e.g., salt and detergent interferences, low protein concentrations) in detecting MTBC.…”

“…19,21 The combination of highpressure high-temperature (HPHT) NDs with TOF mass spectrometry can also effectively detect proteins, 22 biopolymers, 19 DNA oligonucleotides, 23 and multiphosphorylated peptides 24 from complex biosamples feasible. 25 Therefore, ND MALDI-TOF MS is a good platform to detect potential biomarkers under critical environments (e.g., salt and detergent interferences, low protein concentrations) in detecting MTBC. We first showed that DND MALDI-TOF MS can enhance the sensitivity and credibility of detecting specific biomarker, CFP-10, which can identify MTBC from MGIT culture media.…”

Nanodiamonds assisted-cloud point extraction for the determination of fluoranthene in river water

“…12 Among their applications one must highlight their use in electrochemical coatings, 13 polymer compositions, 14 anticorrosion coatings 15 and lubricants, 16 although their most widely described applications are in the biology eld, where they are used as, nanobioprobes to visualize protein-bacteria interactions, 17 carriers of biomolecules in column chromatography, 18 drug carriers, 19 or platforms to extract and elute peptides, proteins, DNA, glycans and other molecules. 20 To date, few analytical applications of nanodiamonds in the sample treatment eld have been described.…”