Application of optical tweezers for biochemical and thermal cell stimulation

Berghoff, Konrad; Keller, Steven H.; Groß, Wolfgang; Gebhardt, Lisa; Kress, Holger

doi:10.1016/b978-0-7020-7096-9.00013-6

Cited by 2 publications

(2 citation statements)

References 76 publications

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“…The area covered by cells was detected using both the DIC and the fluorescence channel simultaneously to obtain robust results. As described previously ( 42 ), a local contrast filter was applied to the DIC images to obtain a rough approximation of the cell mask M DIC . The local contrast filter highlights areas in which the difference ∆ I between the local intensity maximum and minimum is larger than a given threshold T DIC

…”

Section: Methodsmentioning

confidence: 99%

“…Next, the mask was smoothed by a Gaussian filter approximation (43) with an SD of 3 px = 0.6 m to reduce pixel noise at the cell boundary. Objects in the background with an area of less than 80 m 2 were excluded, which appear in M mainly because of noise in the background (42). In this step, care was taken not to exclude any cells ( fig.…”

Section: Quantification Of Pci and Area Covered With Cells On Coverslipsmentioning

confidence: 99%

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Environmental exposure enhances the internalization of microplastic particles into cells

et al. 2020

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Microplastic particles ubiquitously found in the environment are ingested by a huge variety of organisms. Subsequently, microplastic particles can translocate from the gastrointestinal tract into the tissues likely by cellular internalization. The reason for cellular internalization is unknown, since this has only been shown for specifically surface-functionalized particles. We show that environmentally exposed microplastic particles were internalized significantly more often than pristine microplastic particles into macrophages. We identified biomolecules forming an eco-corona on the surface of microplastic particles, suggesting that environmental exposure promotes the cellular internalization of microplastics. Our findings further indicate that cellular internalization is a key route by which microplastic particles translocate into tissues, where they may cause toxicological effects that have implications for the environment and human health.

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…”

Section: Methodsmentioning

confidence: 99%

Section: Quantification Of Pci and Area Covered With Cells On Coverslipsmentioning

confidence: 99%

Environmental exposure enhances the internalization of microplastic particles into cells

et al. 2020

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Phagosomal transport depends strongly on phagosome size

Keller

Berghoff

Kress

2017

Sci Rep

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Macrophages internalize pathogens for intracellular degradation. An important part of this process is the phagosomal transport from the cell periphery to the perinuclear region. Biochemical factors are known to influence the fate of phagosomes. Here, we show that the size of phagosomes also has a strong influence on their transport. We found that large phagosomes are transported persistently to the nucleus, whereas small phagosomes show strong bidirectional transport. We show that dynein motors play a larger role in the transport of large phagosomes, whereas actin filament-based motility plays a larger role in the transport of small phagosomes. Furthermore, we investigated the spatial distribution of dyneins and microtubules around phagosomes and hypothesize that dynein and microtubule density differences between the nucleus-facing side of phagosomes and the opposite side could explain part of the observed transport characteristics. Our findings suggest that a size-dependent cellular sorting mechanism might exist that supports macrophages in their immunological roles.

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Application of optical tweezers for biochemical and thermal cell stimulation

Cited by 2 publications

References 76 publications

Environmental exposure enhances the internalization of microplastic particles into cells

Environmental exposure enhances the internalization of microplastic particles into cells

Phagosomal transport depends strongly on phagosome size

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