Application of organosilicate matrix based on methyltriethoxysilane, PVA and bacteria Paracoccus yeei to create a highly sensitive BOD

Kamanina, Olga A.; Arlyapov, V. A.; Rybochkin, Pavel V.; Lavrova, D. G.; Podsevalova, Elena N.; Понаморева, О. Н.

doi:10.1007/s13205-021-02863-z

Cited by 7 publications

(6 citation statements)

References 35 publications

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“…hansenii VKM Y-2482 was chosen as a suitable biomaterial for BOD biosensors due to its ability to oxidize a wide range of substrates. The utilization of sol–gel technology allows for the formation of organosilicon matrices around microorganism cells, providing protective properties against metal ions and fluctuations in acidity. ,, These inherent advantages enable the development of a biohybrid material characterized by broad substrate specificity, stable responses, and enhanced resistance to environmental stressors when compared to biomaterials composed of a single microorganism strain.…”

Section: Resultsmentioning

confidence: 99%

“3-in-1” Hybrid Biocatalysts: Association of Yeast Cells Immobilized in a Sol–Gel Matrix for Determining Sewage Pollution

Kamanina,

Lantsova,

Rybochkin

et al. 2023

ACS Appl. Mater. Interfaces

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This study presents a novel ″3-in-1″ hybrid biocatalyst design that combines the individual efficiency of microorganisms while avoiding negative interactions between them. Yeast cells of Ogataea polymorpha VKM Y-2559, Blastobotrys adeninivorans VKM Y-2677, and Debaryomyces hansenii VKM Y-2482 were immobilized in an organosilicon material by using the sol–gel method, resulting in a hybrid biocatalyst. The catalytic activity of the immobilized microorganism mixture was evaluated by employing it as the bioreceptor element of a biosensor. Optical and scanning electron microscopies were used to examine the morphology of the biohybrid material. Elemental distribution analysis confirmed the encapsulation of yeast cells in a matrix composed of methyltriethoxysilane (MTES) and tetraethoxysilane (TEOS) (85 and 15 vol %, respectively). The resulting heterogeneous biocatalyst exhibited excellent performance in determining the biochemical oxygen demand (BOD) index in real surface water samples, with a sensitivity coefficient of 50 ± 3 × 10–3·min–1, a concentration range of 0.3–31 mg/L, long-term stability for 25 days, and a relative standard deviation of 3.8%. These findings demonstrate the potential of the developed hybrid biocatalyst for effective pollution monitoring and wastewater treatment applications.

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Section: Resultsmentioning

confidence: 99%

“3-in-1” Hybrid Biocatalysts: Association of Yeast Cells Immobilized in a Sol–Gel Matrix for Determining Sewage Pollution

Kamanina,

Lantsova,

Rybochkin

et al. 2023

ACS Appl. Mater. Interfaces

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“…Previously, we have shown that bacterial cells have the best catalytic activity when immobilized in a sol–gel matrix containing 50 vol.% hydrophobic additive (MTES) and 50 vol.% TEOS [ 31 ]. Therefore, for this work, we chose a ratio of 50 vol.% DEDMS and 50 vol.% TEOS.…”

Section: Resultsmentioning

confidence: 99%

The Use of Diethoxydimethylsilane as the Basis of a Hybrid Organosilicon Material for the Production of Biosensitive Membranes for Sensory Devices

et al. 2022

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Biomembranes based on an organosilica sol–gel matrix were used to immobilize bacteria Paracoccus yeei VKM B-3302 as part of a biochemical oxygen demand (BOD) biosensor. Diethoxydimethylsilane (DEDMS) and tetraethoxysilane (TEOS) were used as precursors to create the matrix in a 1:1 volume ratio. The use of scanning electron microscopy (SEM) and the low-temperature nitrogen adsorption method (BET) showed that the sol–gel matrix forms a capsule around microorganisms that does not prevent the exchange of substrates and waste products of bacteria to the cells. The use of DEDMS as part of the matrix made it possible to increase the sensitivity coefficient of the biosensor for determining BOD by two orders of magnitude compared to a biosensor based on methyltriethoxysilane (MTES). Additionally, the long-term stability of the bioreceptor increased to 68 days. The use of such a matrix neutralized the effect of heavy metal ions on the microorganisms’ catalytic activity in the biosensor. The developed biosensor was used to analyze water samples from water sources in the Tula region (Russia).

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“…The immobilization of bacteria in a sol-gel matrix leads to stabilization of the catalytic activity and makes it possible to repeatedly or continuously use the biocatalyst. The integration of microorganisms into sol-gel structures removes many limitations that arise during the working with free cell systems [ 21 , 40 , 41 , 42 ].…”

Section: The Classification Of Hybrid Materials According To the Type...mentioning

confidence: 99%

Preparation of Hybrid Sol-Gel Materials Based on Living Cells of Microorganisms and Their Application in Nanotechnology

et al. 2022

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Microorganism-cell-based biohybrid materials have attracted considerable attention over the last several decades. They are applied in a broad spectrum of areas, such as nanotechnologies, environmental biotechnology, biomedicine, synthetic chemistry, and bioelectronics. Sol-gel technology allows us to obtain a wide range of high-purity materials from nanopowders to thin-film coatings with high efficiency and low cost, which makes it one of the preferred techniques for creating organic-inorganic matrices for biocomponent immobilization. This review focuses on the synthesis and application of hybrid sol-gel materials obtained by encapsulation of microorganism cells in an inorganic matrix based on silicon, aluminum, and transition metals. The type of immobilized cells, precursors used, types of nanomaterials obtained, and their practical applications were analyzed in detail. In addition, techniques for increasing the microorganism effective time of functioning and the possibility of using sol-gel hybrid materials in catalysis are discussed.

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Application of organosilicate matrix based on methyltriethoxysilane, PVA and bacteria Paracoccus yeei to create a highly sensitive BOD

Cited by 7 publications

References 35 publications

“3-in-1” Hybrid Biocatalysts: Association of Yeast Cells Immobilized in a Sol–Gel Matrix for Determining Sewage Pollution

“3-in-1” Hybrid Biocatalysts: Association of Yeast Cells Immobilized in a Sol–Gel Matrix for Determining Sewage Pollution

The Use of Diethoxydimethylsilane as the Basis of a Hybrid Organosilicon Material for the Production of Biosensitive Membranes for Sensory Devices

Preparation of Hybrid Sol-Gel Materials Based on Living Cells of Microorganisms and Their Application in Nanotechnology

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