Application of physico-chemical typing methods for the epidemiological analysis of<i>Salmonella enteritidis</i>strains of phage type 25/17

Seltmann, G.; Voigt, W.; Beer, W.

doi:10.1017/s0950268800068424

Cited by 33 publications

(12 citation statements)

References 17 publications

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“…FT-IR spectroscopy has been used in combination with other techniques for qualitative studies on intraspecific diversity (Seltmann et al, 1994(Seltmann et al, , 1995Irmscher et al, 1999 ;Stan-Lotter et al, 1999), but, as far as we are aware, the method has not previously been applied to quantitative assessment of intraspecific diversity, i.e. to quantification of the degree of heterogeneity.…”

Section: Abbreviation : Ft-ir Fourier-transform Infraredmentioning

confidence: 99%

Intraspecific diversity of Brevibacterium linens, Corynebacterium glutamicum and Rhodococcus erythropolis based on partial 16S rDNA sequence analysis and Fourier-transform infrared (FT-IR) spectroscopy The GenBank accession numbers for the 16S rDNA gene sequences reported in this paper are AY017065 to AY017067, AY017069 to AY017087, and AF426135 to AF426143 for Brevibacterium linens; AY017088 to AY017091, AY017093 to AY017104, AY017107 to AY017111, and AF426144 to AF426149 for Corynebacterium glutamicum; a

2002

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The intraspecific diversity of 31 strains of Brevibacterium linens, 27 strains of Corynebacterium glutamicum and 29 strains of Rhodococcus erythropolis was determined by partial 16S rDNA sequence analysis and Fourier-transform infrared (FT-IR) spectroscopy. As a prerequisite for the analyses, 27 strains derived from culture collections which had carried invalid or wrong species designations were reclassified in accordance with polyphasic taxonomical data. FT-IR spectroscopy proved to be a rapid and reliable method for screening for similar isolates and for identifying these actinomycetes at the species level. Two main conclusions emerged from the analyses.

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Section: Abbreviation : Ft-ir Fourier-transform Infraredmentioning

confidence: 99%

Intraspecific diversity of Brevibacterium linens, Corynebacterium glutamicum and Rhodococcus erythropolis based on partial 16S rDNA sequence analysis and Fourier-transform infrared (FT-IR) spectroscopy The GenBank accession numbers for the 16S rDNA gene sequences reported in this paper are AY017065 to AY017067, AY017069 to AY017087, and AF426135 to AF426143 for Brevibacterium linens; AY017088 to AY017091, AY017093 to AY017104, AY017107 to AY017111, and AF426144 to AF426149 for Corynebacterium glutamicum; a

2002

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“…These studies have shown that it is possible to discriminate among various microorganisms at the genus, species, and strain level (14, 21-22, 25, 27, 29-32, 34, 49), and studies report the ability to differentiate microorganisms from various serogroups (21)(22)(23)37). Furthermore, the use of FT-IR spectroscopy to identify drug resistance has also been investigated (3,42).…”

mentioning

confidence: 99%

Investigating Microbial (Micro)colony Heterogeneity by Vibrational Spectroscopy

Choo-Smith

Maquelin

Vreeswijk

et al. 2001

Appl Environ Microbiol

231

160

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Fourier transform infrared and Raman microspectroscopy are currently being developed as new methods for the rapid identification of clinically relevant microorganisms. These methods involve measuring spectra from microcolonies which have been cultured for as little as 6 h, followed by the nonsubjective identification of microorganisms through the use of multivariate statistical analyses. To examine the biological heterogeneity of microorganism growth which is reflected in the spectra, measurements were acquired from various positions within (micro)colonies cultured for 6, 12, and 24 h. The studies reveal that there is little spectral variance in 6-h microcolonies. In contrast, the 12-and 24-h cultures exhibited a significant amount of heterogeneity. Hierarchical cluster analysis of the spectra from the various positions and depths reveals the presence of different layers in the colonies. Further analysis indicates that spectra acquired from the surface of the colonies exhibit higher levels of glycogen than do the deeper layers of the colony. Additionally, the spectra from the deeper layers present with higher RNA levels than the surface layers. Therefore, the 6-h colonies with their limited heterogeneity are more suitable for inclusion in a spectral database to be used for classification purposes. These results also demonstrate that vibrational spectroscopic techniques can be useful tools for studying the nature of colony development and biofilm formation.In recent years, there has been much effort invested into the development of new techniques for the identification of microorganisms. Many of these methods are aimed at providing the clinician with more rapid identification of the microorganism responsible for infection in order to begin the appropriate course of antimicrobial treatment (1,9,15,21,27,31,44,51). The emergence of these novel methods reflects the rise in drug-resistant microorganisms, which requires that antimicrobial treatment be more effectively managed (2, 12, 28, 52). Among the new methods are those based on vibrational spectroscopic techniques, namely Fourier transform infrared (FT-IR) and Raman spectroscopies. Vibrational spectroscopic methods are reagentless procedures in which there is no need to add dyes or labels for spectral measurement. These nondestructive techniques are based on the absorption (FT-IR) or scattering (Raman) of light directed onto a sample. The amount of light absorbed or scattered depends on the molecules found within the sample and the environment in which these molecules are found. With these highly sensitive techniques, the frequency of light in the resulting spectrum provides biochemical information regarding the molecular composition and molecular structure of and molecular interaction in cells and tissues (24,55). Raman and infrared spectroscopies are complementary techniques which together can provide a more complete impression of the biochemical information within a sample. Furthermore, these two methods differ such that each is capable of providing informatio...

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“…The patterns of the O-antigenic lipopolysaccharides (LPS) and the OMPs as well as the whole-cell protein profiles (WCPPs) were determined by SDS-PAGE as described earlier (22). MLEE was performed in a starch gel under nondenaturing conditions as described previously.…”

Section: Methodsmentioning

confidence: 99%