Application of PLGA-collagen hybrid mesh for three-dimensional culture of canine anterior cruciate ligament cells

Chen, Guoping; Sato, Takashi; Sakane, Masataka; Ohgushi, Hajime; Ushida, Takashi; Tanaka, Junzo; Tateishi, Tetsuya

doi:10.1016/j.msec.2004.08.041

Cited by 26 publications

(15 citation statements)

References 29 publications

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“…The hydrophilic property of collagen in the collagen/PLGAcollagen biphasic sponge facilitated cell seeding. In our previous study, we hybridized synthetic polymer scaffolds with collagen sponge to improve cell-seeding efficiency, and thus promote tissue regeneration [25][26][27][28]. The same effect was achieved in this study.…”

Section: Discussionsupporting

confidence: 72%

Preparation of a biphasic scaffold for osteochondral tissue engineering

Chen

Sato

Tanaka

et al. 2006

Materials Science and Engineering: C

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Section: Discussionsupporting

confidence: 72%

Preparation of a biphasic scaffold for osteochondral tissue engineering

Chen

Sato

Tanaka

et al. 2006

Materials Science and Engineering: C

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“…ACL fibroblasts were isolated by collagenase digestion as described elsewhere (30). For these experiments, ACL were harvested from four patients (three males, 61, 66 and 77 years old, respectively, and one 70-year-old female); the bony attachment sites and synovial sheath were removed, and the ligament fascicles were dissected, minced and digested with 0.1% (w/v) collagenase type I solution (Serva Electrophoresis GmbH, Heidelberg, Germany) for 18 h. ACL fibroblasts were recovered by filtration through a 70-μm nylon mesh cell strainer (Falcon, Becton Dickinson Labware, Franklin Lakes, NJ, USA) and centrifuged at 1500 r.p.m.…”

Section: Methodsmentioning

confidence: 99%

BMP12 and BMP13 gene transfer induce ligamentogenic differentiation in mesenchymal progenitor and anterior cruciate ligament cells

Haddad-Weber¹,

Prager²,

Kunz³

et al. 2010

Cytotherapy

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Background aims To date there are only very few data available on the ligamentogenic differentiation capacity of mesenchymal stromal/progenitor cells (MSC) and anterior cruciate ligament (ACL) fibroblasts. Methods We describe the in vitro potential of MSC and ACL cells to undergo ligamentogenic differentiation upon transduction with adenoviral vectors encoding the human cDNA for bone morphogenetic protein (BMP) 12 and BMP13, also known as growth and differentiation factors (GDF) 6 and 7, respectively. Results Transgene expression for at least 14 days was confirmed by Western blot analyzes. After 21 days of cell culture within collagen type I hydrogels, histochemical (hematoxylin/eosin (H&E), Azan and van Gieson), immunohistochemical and polymerase chain reaction (PCR) analyzes of the genetically modified constructs of both cell types revealed elongated, viable fibroblast-like cells embedded in a ligament-like matrix rich in collagens, vimentin, fibronectin, decorin, elastin, scleraxis, tenascin, and tenomodulin. Conclusions It appears that both MSC and ACL fibroblasts are capable of ligamentogenic differentiation with these factors. This information may aid in the development of biologic approaches to repair and restore ACL after injury.

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“…However, the PLGA scaffold has the problems of surface hydrophobicity and limited cell adhesion. One modified knitted PLGA mesh hybridized with collagen has been investigated as a three‐dimensional culture system for skin tissue engineering85 or some other tissues construction 87,88. These web‐like collagen sponges can facilitate seeding, uniform distribution, and ECM synthesis of dermal fibroblasts (Figure 4D) 85.…”

Section: Current Materials and Scaffolds In Wound Healingmentioning

confidence: 99%

Tissue scaffolds for skin wound healing and dermal reconstruction

Zhong

Zhang

Lim

2010

WIREs Nanomed Nanobiotechnol

579

409

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One of the major applications of tissue-engineered skin substitutes for wound healing is to promote the healing of cutaneous wounds. In this respect, many important clinical milestones have been reached in the past decades. However, currently available skin substitutes for wound healing often suffer from a range of problems including wound contraction, scar formation, and poor integration with host tissue. Engineering skin substitutes by tissue engineering approach has relied upon the creation of three-dimensional scaffolds as extracellular matrix (ECM) analog to guide cell adhesion, growth, and differentiation to form skin-functional and structural tissue. The three-dimensional scaffolds can not only cover wound and give a physical barrier against external infection as wound dressing, but also can provide support both for dermal fibroblasts and the overlying keratinocytes for skin tissue engineering. A successful tissue scaffold should exhibit appropriate physical and mechanical characteristics and provide an appropriate surface chemistry and nano and microstructures to facilitate cellular attachment, proliferation, and differentiation. A variety of scaffolds have been fabricated based on materials ranging from naturally occurring ones to those manufactured synthetically. This review discusses a variety of commercial or laboratory-engineered skin substitutes for wound healing. Central to the discussion are the scaffolds/materials, fabrication techniques, and their characteristics associated with wound healing. One specifically highlighted emerging fabrication technique is electrospinning that allows the design and fabrication of biomimetic scaffolds that offer tremendous potential applications in wound healing of skin.

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Application of PLGA-collagen hybrid mesh for three-dimensional culture of canine anterior cruciate ligament cells

Cited by 26 publications

References 29 publications

Preparation of a biphasic scaffold for osteochondral tissue engineering

Preparation of a biphasic scaffold for osteochondral tissue engineering

BMP12 and BMP13 gene transfer induce ligamentogenic differentiation in mesenchymal progenitor and anterior cruciate ligament cells

Tissue scaffolds for skin wound healing and dermal reconstruction

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