Preparation of a biphasic scaffold for osteochondral tissue engineering

Chen, Guoping; Sato, Takashi; Tanaka, Junzo; Tateishi, Tetsuya

doi:10.1016/j.msec.2005.07.024

Cited by 87 publications

(57 citation statements)

References 31 publications

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“…Common approaches involve: 6 (1) seeding autologous chondrocytes at the top of the scaffold creating a cell-scaffold construct for in vivo implantation, [18][19][20][21] (2) two different cartilage and bone scaffolds assembled together either before or during implantation, 22,23 and (3) an integrated bilayered composite structure that leads to a complete integration of bone and cartilage layers without needing a subsequent joining mechanism. [24][25][26] Moreover, several strategies with single-layer materials have been put forward, as reviewed by Mano and Reis. 3 It is accepted that bilayered structures are more challenging to design and fabricate but they are ultimately more suitable for regenerating osteochondral defects.…”

Section: Introductionmentioning

confidence: 99%

Stratified scaffolds for osteochondral tissue engineering applications: Electrospun PDLLA nanofibre coated Bioglass®-derived foams

et al. 2011

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This work focuses on designing bilayered constructs by combining electrospun poly-DL-Lactide (PDLLA) fibers and Bioglass Õ -derived scaffolds for development of osteochondral tissue replacement materials. Electrospinning was carried out using a solution of 5 wt/v% PDLLA in dimethyl carbonate. The PDLLA layer thickness increased from 2 to 150 mm with varying electrospinning time. Bioactivity studies in simulated body fluid showed that HA mineralization decreased as the thickness of the PDLLA layer increased. A preliminary in vitro study using chondrocyte cells (ATDC5) showed that cells attached, proliferated and migrated into the fibrous network, confirming the potential applicability of the bilayered scaffolds in osteochondral defect regeneration.

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Section: Introductionmentioning

confidence: 99%

Stratified scaffolds for osteochondral tissue engineering applications: Electrospun PDLLA nanofibre coated Bioglass®-derived foams

et al. 2011

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“…Poly(lactic-co-glycolic acid) (PLGA) is bioabsorbable and biocompatible material, making it a promising material in the context of regenerative medicine. Numerous attempts have been made for successful tissue reconstruction using PLGAbased scaffold, either by PLGA itself Uematsu et al, 2005) or by incorporation of PLGA with natural polymers such as collagen (Chen et al, 2006a;Chen et al, 2006b), extracellular matrix scaffolds -namely small intestinal submucosa Lee et al, 2004) and demineralised bone particles (Jang et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

The use of fibrin and poly(lactic-co-glycolic acid) hybrid scaffold for articular cartilage tissue engineering: an in vivo analysis

Munirah¹,

Sh²,

Ruszymah³

et al. 2008

eCM

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Our preliminary results indicated that fibrin and poly(lacticco-glycolic acid) (PLGA) hybrid scaffold promoted early chondrogenesis of articular cartilage constructs in vitro. The aim of this study was to evaluate in vivo cartilaginous tissue formation by chondrocyte-seeded fibrin/PLGA hybrid scaffolds. PLGA scaffolds were soaked carefully, in chondrocyte-fibrin suspension, and polymerized by dropping thrombin-calcium chloride (CaCl 2 ) solution. PLGA-seeded chondrocytes were used as a control. Resulting constructs were implanted subcutaneously, at the dorsum of nude mice, for 4 weeks. Macroscopic observation, histological evaluation, gene expression and sulphated-glycosaminoglycan (sGAG) analyses were performed at each time point of 1, 2 and 4 weeks postimplantation. Cartilaginous tissue formation in fibrin/PLGA hybrid construct was confirmed by the presence of lacunae and cartilage-isolated cells embedded within basophilic ground substance. Presence of proteoglycan and glycosaminoglycan (GAG) in fibrin/PLGA hybrid constructs was confirmed by positive Safranin O and Alcian Blue staining. Collagen type II exhibited intense immunopositivity at the pericellular matrices. Chondrogenic properties were further demonstrated by the expression of gene encoded cartilage-specific markers, collagen type II and aggrecan core protein. The sGAG production in fibrin/PLGA hybrid constructs was higher than in the PLGA group. In conclusion, fibrin/PLGA hybrid scaffold promotes cartilaginous tissue formation in vivo and may serve as a potential cell delivery vehicle and a structural basis for articular cartilage tissue-engineering.

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“…Osteochondral tissue was regenerated four months after implantation in the knee of a one-year beagle. Previously canine bone-marrow-derived mesenchymal stem cells were cultivated in the biphasic scaffold (86). Ghosh et al, fabricated bi-layerded construct based on poly(L-lactic acid) and starch for osteocondral defect.…”

Section: Layered Scaffolds For Osteochondral Te: An Overviewmentioning

confidence: 99%

“…The evolution of the mass of the samples as a function of the degradation time was determined following equation 7, p. 86. Figure 3-30 shows the mass residue of the different scaffolds after various times of degradation.…”

Section: Mass Evolutionmentioning

confidence: 99%

Scaffold design and characterisation for osteochondral tissue regeneration

Deplaine¹

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Preparation of a biphasic scaffold for osteochondral tissue engineering

Cited by 87 publications

References 31 publications

Stratified scaffolds for osteochondral tissue engineering applications: Electrospun PDLLA nanofibre coated Bioglass®-derived foams

Stratified scaffolds for osteochondral tissue engineering applications: Electrospun PDLLA nanofibre coated Bioglass®-derived foams

The use of fibrin and poly(lactic-co-glycolic acid) hybrid scaffold for articular cartilage tissue engineering: an in vivo analysis

Scaffold design and characterisation for osteochondral tissue regeneration

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