Application of Polydopamine Functionalized Zinc Oxide for Glucose Biosensor Design

Abstract

Zinc oxide (ZnO) nanostructures are widely used in optical sensors and biosensors. Functionalization of these nanostructures with polymers enables optical properties of ZnO to be tailored. Polydopamine (PDA) is a highly biocompatible polymer, which can be used as a versatile coating suitable for application in sensor and biosensor design. In this research, we have grown ZnO-based nanorods on the surface of ITO-modified glass-plated optically transparent electrodes (glass/ITO). Then the deposition of the PDA polymer layer on the surface of ZnO nanorods was performed from an aqueous PDA solution in such a way glass/ITO/ZnO-PDA structure was formed. The ZnO-PDA composite was characterized by SEM, TEM, and FTIR spectroscopy. Then glucose oxidase (GOx) was immobilized using crosslinking by glutaraldehyde on the surface of the ZnO-PDA composite, and glass/ITO/ZnO-PDA/GOx-based biosensing structure was designed. This structure was applied for the photo-electrochemical determination of glucose (Glc) in aqueous solutions. Photo-electrochemical determination of glucose by cyclic voltammetry and amperometry has been performed by glass/ITO/ZnO-PDA/GOx-based biosensor. Here reported modification/functionalization of ZnO nanorods with PDA enhances the photo-electrochemical performance of ZnO nanorods, which is well suited for the design of photo-electrochemical sensors and biosensors.

Polydopamine Coated CeO2 as Radical Scavenger Filler for Aquivion Membranes with High Proton Conductivity

“…The most stable membrane was also characterized by Open Circuit Voltage (OCV) stress tests coupled with hydrogen crossover determinations. The preparation of PDA-based materials has rapidly advanced in recent years with a significant expansion in their applications [21][22][23][24][25][26][27][28][29][30][31][32][33][34], becoming one of the most attractive areas within the materials field including surface modification, biosensing [35,36], nanomedicine [37] and systems for energy applications [38][39][40][41][42][43]. In particular, PDA allows obtainment of a beneficial and advantageous interface between CeO 2 and PFSA improving the lifetime of PEM fuel cells [44].…”

Photoelectrochemical Enzyme Biosensor Based on TiO2 Nanorod/TiO2 Quantum Dot/Polydopamine/Glucose Oxidase Composites with Strong Visible-Light Response

“…Polydopamine (PDA) exhibits good biocompatibility, high adhesive strength, and a wide absorption range and has therefore often been used to modify the photoelectrochemically active material. 14,15 Herein, we developed a PEC enzyme biosensor based on PDA-assisted visible-light excitation and used the device to detect glucose. TiO 2 QDs were grown uniformly in situ on the surface of TiO 2 NRs through a simple hydrothermal reaction.…”

Effect of Electrode Modification with Chitosan and Nafion® on the Efficiency of Real-Time Enzyme Glucose Biosensors Based on ZnO Tetrapods

“…The functionalization strategy of different transducers (including ZnO) usually requires several consequent steps to be performed. At the initial step, ZnO is treated with various cross-linking agents (e.g., glutaraldehyde) [1,26], at the second-glucose oxidase (GOx) deposition [27], and at the final-deposition of a polymer layer [28][29][30]. The last one plays an essential role, as it simultaneously performs several important functions: (i) prevents of GOx from leaking out, (ii) permits charge transfer through it, and (iii) reduces interference by ionic substances [31].…”