Application of polyethyleneglycol (PEG)-modified liposomes for oral vaccine: effect of lipid dose on systemic and mucosal immunity

Minato, Seiichiro; Iwanaga, Kazunori; Kakemi, Masawo; Yamashita, Shinji; Oku, Naoto

doi:10.1016/s0168-3659(03)00093-2

Cited by 70 publications

(34 citation statements)

References 21 publications

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“…Alguns vírus são direcionados para células ou tecidos específicos: Epstein-Barr vírus (EBV) o qual infecta principalmente linfócitos B, vírus da hepatite B, que ataca os hepatócitos, e HIV direcionados para os linfócitos e macrófagos (Kaneda, 2000). Minato et al (2003) realizaram um estudo avaliando o efeito do uso de lipossomas PEG-modificados na administração oral de vacina utilizando ovalbumina como modelo de antígeno. Concluíram que lipossomas peguilados melhoram a imunidade da mucosa, provavelmente devido à liberação controlada de antígenos dos lipossomas para a mucosa local.…”

Section: Lipossomas No Desenvolvimento De Vacinasunclassified

Lipossomas e suas aplicações terapêuticas: estado da arte

Batista¹,

Carvalho²,

Magalhães³

2007

Rev. Bras. Cienc. Farm.

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Lipossomas são vesículas constituídas de uma ou mais bicamadas INTRODUÇÃOAlec Bangham há 40 anos com sua observação pioneira de que fosfolipídeos em soluções aquosas podem formar estruturas fechadas em bicamadas, permitiu que a pesquisa sobre os lipossomas percorresse um longo caminho, convertendo-os de simples objetos de pesquisa biofísica em carreadores terapêuticos para numerosas aplicações clínicas (Torchilin, 2005).Os primeiros resultados das pesquisas foram, entre-

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Section: Lipossomas No Desenvolvimento De Vacinasunclassified

Lipossomas e suas aplicações terapêuticas: estado da arte

Batista¹,

Carvalho²,

Magalhães³

2007

Rev. Bras. Cienc. Farm.

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“…The effective induction of these responses can protect the body from the majority of pathogens. However, oral vaccination mostly fails to induce the protective mucosal immune responses [Ponchel et al, 1998;Minato et al, 2003] since orally administrated vaccines are readily degraded in the gastrointestinal (GI) tract with inefficient targeting to the site of action in the gutassociated lymphoid tissue (GALT) [Zhou et al, 2002]. To improve their delivery efficiency to the site of action and to protect vaccines against the GI tract, particulate techniques to encapsulate vaccines have been used frequently.…”

Section: Introductionmentioning

confidence: 99%

“…Chitosan can be used as a delivery system to achieve site-specific delivery of proteins or peptides to the active site and is of considerable interest in bioadhesive drug delivery systems due to its mucoadhesive property, biocompatibility, biodegradibility, and low toxicity [Janes et al, 2001;Lehr et al, 1992]. Liposomes taken up by the PPs following oral administration have potential for developing oral vaccines [Minato et al, 2003;Zhou et al, 2002].…”

Section: Introductionmentioning

confidence: 99%

Radioevaluation of PAMs, CMs, and PS‐Lip as an oral carrier for vaccine delivery into intestinal Peyer's patches

Lee

Heo

Song

et al. 2006

Drug Development Research

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The aim of the present study was to evaluate the utility of pullulan acetate microparticles (PAMs), chitosan micropaticles (CMs), and dipalmitoylphosphatidyl-serine-liposomes (PS-Lip) as oral carriers for delivery to the intestinal Peyer's patches (PPs). To monitor PP delivery after oral administration, PAMs, CMs, and PS-Lip were radiolabeled with 99m Tc. Radiolabeling efficiencies of the particles were 9572.5% (PAMs), 8774.3% (CMs), and 77.275.8% (PS-Lip). In delivery studies to the PPs, the percentage of PS-Lip taken up to the PPs was 3.8 Â 10 À3 70.3% of the administered dose with PS-Lip group showed significantly high uptake compared to the PAM and CM groups. These results suggest that PS-Lip may be used as a potential system for developing an oral delivery carrier. Drug Dev. Res. 67:884-889, 2006.

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“…[7][8][9] The most frequently used PEGylation method has been reported to produce stable liposomes and enhance the oral bioavailability of poorly soluble and low-bioavailability drugs. 10,11 In these cases, the poly(ethylene glycol)s (PEGs) were chemically linked to the hydrophilic amino termini of phospholipids, forming phospholipid-PEG conjugates for physical liposomal structural stabilization and efficient steric protection. Similar methods have been used to construct oral liposomes, such as polysaccharide-anchored liposomes using synthesized glycolipids.…”

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confidence: 99%

Silica-coated flexible liposomes as a nanohybrid delivery system for enhanced oral bioavailability of curcumin

Li¹,

Zhang²,

Su³

et al. 2012

IJN

107

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Abstract:We investigated flexible liposomes as a potential oral drug delivery system. However, enhanced membrane fluidity and structural deformability may necessitate liposomal surface modification when facing the harsh environment of the gastrointestinal tract. In the present study, silica-coated flexible liposomes loaded with curcumin (CUR-SLs) having poor water solubility as a model drug were prepared by a thin-film method with homogenization, followed by the formation of a silica shell by the sol-gel process. We systematically investigated the physical properties, drug release behavior, pharmacodynamics, and bioavailability of CUR-SLs. CURSLs had a mean diameter of 157 nm and a polydispersity index of 0.14, while the apparent entrapment efficiency was 90.62%. Compared with curcumin-loaded flexible liposomes (CURFLs) without silica-coatings, CUR-SLs had significantly higher stability against artificial gastric fluid and showed more sustained drug release in artificial intestinal fluid as determined by in vitro release assays. The bioavailability of CUR-SLs and CUR-FLs was 7.76-and 2.35-fold higher, respectively, than that of curcumin suspensions. Silica coating markedly improved the stability of flexible liposomes, and CUR-SLs exhibited a 3.31-fold increase in bioavailability compared with CUR-FLs, indicating that silica-coated flexible liposomes may be employed as a potential carrier to deliver drugs with poor water solubility via the oral route with improved bioavailability.

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Application of polyethyleneglycol (PEG)-modified liposomes for oral vaccine: effect of lipid dose on systemic and mucosal immunity

Cited by 70 publications

References 21 publications

Lipossomas e suas aplicações terapêuticas: estado da arte

Lipossomas e suas aplicações terapêuticas: estado da arte

Radioevaluation of PAMs, CMs, and PS‐Lip as an oral carrier for vaccine delivery into intestinal Peyer's patches

Silica-coated flexible liposomes as a nanohybrid delivery system for enhanced oral bioavailability of curcumin

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